Logistic regression analysis of factors associated with pentafecta achievement after robot-assisted partial nephrectomy on T1b patients.

<p>Logistic regression analysis of factors associated with pentafecta achievement after robot-assisted partial nephrectomy on T1b patients.</p

Logistic regression analysis of factors associated with trifecta achievement after robot-assisted partial nephrectomy on T1b patients

<p>Logistic regression analysis of factors associated with trifecta achievement after robot-assisted partial nephrectomy on T1b patients</p

Clinical characteristics of patients with T1a, T1b renal mass.

<p>Clinical characteristics of patients with T1a, T1b renal mass.</p

Comparison of contemporary reports on trifecta, pentafecta results of robot-assisted partial nephrectomy.

<p>Comparison of contemporary reports on trifecta, pentafecta results of robot-assisted partial nephrectomy.</p

Oncological, perioperative, functional outcomes after robot-assisted partial nephrectomy.

<p>Oncological, perioperative, functional outcomes after robot-assisted partial nephrectomy.</p

Hyper-Enhanced Production of Foreign Recombinant Protein by Fusion with the Partial Polyhedrin of Nucleopolyhedrovirus

<div><p>To enhance the production efficiency of foreign protein in baculovirus expression systems, the effects of polyhedrin fragments were investigated by fusion expressing them with the enhanced green fluorescent protein (EGFP). Recombinant viruses were generated to express EGFP fused with polyhedrin fragments based on the previously reported minimal region for self-assembly and the KRKK nuclear localization signal (NLS). Fusion expressions with polyhedrin amino acids 19 to 110 and 32 to 110 lead to localization of recombinant protein into the nucleus and mediate its assembly. The marked increase of EGFP by these fusion expressions was confirmed through protein and fluorescence intensity analyses. The importance of nuclear localization for enhanced production was shown by the mutation of the NLS within the fused polyhedrin fragment. In addition, when the polyhedrin fragment fused with EGFP was not localized in the nucleus, some fragments increased the production of protein. Among these fragments, some degradation of only the fused polyhedrin was observed in the fusion of amino acids 19 to 85 and 32 to 85. The fusion of amino acids 32 to 85 may be more useful for the enhanced and intact production of recombinant protein. The production of E2 protein, which is a major antigen of classical swine fever virus, was dramatically increased by fusion expression with polyhedrin amino acids 19 to 110, and its preliminary immunogenicity was verified using experimental guinea pigs. This study suggests a new option for higher expression of useful foreign recombinant protein by using the partial polyhedrin in baculovirus.</p> </div

Expression of CSFV E2 protein fused with partial polyhedrin.

<p>Sf21 cells were infected at an MOI of 5 with each virus and harvested at 4 days post-infection. Protein samples were analyzed by SDS-PAGE (A) and Western blot analysis with E2 monoclonal antibody (B).</p

Primers used for the amplification and sequencing in this study.

a<p>For each truncation construct, forward primer (F) is in the forward orientation relative to the coding strand, and reverse primer (R) is in the reverse orientation relative to the coding strand.</p>b<p>Restriction enzyme site shown in bold; Kozak consensus translation initiation sequence shown in italics.</p

Blocking rate of E2 antibodies induced in the sera of guinea pigs immunized with rAc-19-110-E2- ΔTMR.

<p>Four guinea pigs were immunized with total cell extracts infected with rAc19-110-E2-ΔTMR and wild type AcMNPV for 4 weeks. The levels of E2-specific antibodies in the sera were measured using the IDEXX CSFV Ab Test Kit. The optical densities of the samples were measured at 450 nm using an ELISA microplate reader. The test samples were considered serologically positive when the blocking percentage was ≥40% (dotted line). The blocking percentages are expressed as the mean ± SE (n = 4).</p

Fluorescence intensity of EGFP.

<p>Sf21 cells were infected at an MOI of 5 with each virus and harvested 3 days post-infection. The fluorescence intensity of the cell extracts was measured using a fluorescence spectrometer (B). The bars indicate the mean ± SE (<i>n</i> = 3).</p