Intracellular Nanomaterial Delivery via Spiral Hydroporation

In recent nanobiotechnology developments, a wide variety of functional nanomaterials and engineered biomolecules have been created, and these have numerous applications in cell biology. For these nanomaterials to fulfill their promises completely, they must be able to reach their biological targets at the subcellular level and with a high level of specificity. Traditionally, either nanocarrier- or membrane disruption-based method has been used to deliver nanomaterials inside cells; however, these methods are suboptimal due to their toxicity, inconsistent delivery, and low throughput, and they are also labor intensive and time-consuming, highlighting the need for development of a next-generation, intracellular delivery system. This study reports on the development of an intracellular nanomaterial delivery platform, based on unexpected cell-deformation phenomena via spiral vortex and vortex breakdown exerted in the cross- and T-junctions at moderate Reynolds numbers. These vortex-induced cell deformation and sequential restoration processes open cell membranes transiently, allowing effective and robust intracellular delivery of nanomaterials in a single step without the aid of carriers or external apparatus. By using the platform described here (termed spiral hydroporator), we demonstrate the delivery of different nanomaterials, including gold nanoparticles (200 nm diameter), functional mesoporous silica nanoparticles (150 nm diameter), dextran (hydrodynamic diameters between 2–55 nm), and mRNA, into different cell types. We demonstrate here that the system is highly efficient (up to 96.5%) with high throughput (up to 1 × 106 cells/min) and rapid delivery (∼1 min) while maintaining high levels of cell viability (up to 94%)

Quantum-dot light-emitting diodes utilizing CdSe/ZnS nanocrystals embedded in TiO(2) thin film

Quantum-dot (QD) light-emitting diodes (LEDs) are demonstrated on Si wafers by embedding core-shell CdSe/ZnS nanocrystals in TiO(2) thin films via plasma-enhanced metallorganic chemical vapor deposition. The n-TiO(2)/QDs/p-Si LED devices show typical p-n diode current-voltage and efficient electroluminescence characteristics, which are critically affected by the removal of QD surface ligands. The TiO(2)/QDs/Si system we presented can offer promising Si-based optoelectronic and electronic device applications utilizing numerous nanocrystals synthesized by colloidal solution chemistry.open181

Detection of orbital and superhump periods in Nova V2574 Ophiuchi (2004)

We present the results of 37 nights of CCD unfiltered photometry of nova V2574 Oph (2004) from 2004 and 2005. We find two periods of 0.14164 d (~3.40 h) and 0.14773 d (~3.55 h) in the 2005 data. The 2004 data show variability on a similar timescale, but no coherent periodicity was found. We suggest that the longer periodicity is the orbital period of the underlying binary system and that the shorter period represents a negative superhump. The 3.40 h period is about 4% shorter than the orbital period and obeys the relation between superhump period deficit and binary period. The detection of superhumps in the light curve is evidence of the presence of a precessing accretion disk in this binary system shortly after the nova outburst. From the maximum magnitude - rate of decline relation, we estimate the decay rate t_2 = 17+/-4 d and a maximum absolute visual magnitude of M_Vmax = -7.7+/-1.7 mag.Comment: 6 pages, 6 figures, 2 .sty files, AJ accepted, minor change to one of reference

A Mechanistic Analysis of Phase Evolution and Hydrogen Storage Behavior in Nanocrystalline Mg(BH4)2 within Reduced Graphene Oxide.

Magnesium borohydride (Mg(BH4)2, abbreviated here MBH) has received tremendous attention as a promising onboard hydrogen storage medium due to its excellent gravimetric and volumetric hydrogen storage capacities. While the polymorphs of MBH-alpha (α), beta (β), and gamma (γ)-have distinct properties, their synthetic homogeneity can be difficult to control, mainly due to their structural complexity and similar thermodynamic properties. Here, we describe an effective approach for obtaining pure polymorphic phases of MBH nanomaterials within a reduced graphene oxide support (abbreviated MBHg) under mild conditions (60-190 °C under mild vacuum, 2 Torr), starting from two distinct samples initially dried under Ar and vacuum. Specifically, we selectively synthesize the thermodynamically stable α phase and metastable β phase from the γ-phase within the temperature range of 150-180 °C. The relevant underlying phase evolution mechanism is elucidated by theoretical thermodynamics and kinetic nucleation modeling. The resulting MBHg composites exhibit structural stability, resistance to oxidation, and partially reversible formation of diverse [BH4]- species during de- and rehydrogenation processes, rendering them intriguing candidates for further optimization toward hydrogen storage applications

Theory of One-Channel vs. Multi-Channel Kondo Effects for Ce3+^{3+} Impurities

We introduce a model for Ce$^{3+}$ impurities in cubic metals which exhibits competition between the Fermi-liquid fixed point of the single channel Kondo model and the non-Fermi-liquid fixed point of the two- and three-channel Kondo models. Using the non-crossing approximation and scaling theory, we find: (i) A possible three-channel Kondo effect between the one- and two-channel regimes in parameter space. (ii) The sign of the thermopower is a fixed point diagnostic. (iii) Our results will likely survive the introduction of additional $f^2$ and conduction states. We apply this model to interpret the non-Fermi liquid alloy La$_{1-x}$Ce$_x$Cu$_{2.2}$Si$_2$.Comment: 13 pages, Revtex, To appear in Phys. Rev. Let

Endogenously produced nonclassical vitamin D hydroxy-metabolites act as "biased" agonists on VDR and inverse agonists on RORα and RORγ

The classical pathway of vitamin D activation follows the sequence D3→25(OH)D3→1,25(OH)(2)D3 with the final product acting on the receptor for vitamin D (VDR). An alternative pathway can be started by the action of CYP11A1 on the side chain of D3, primarily producing 20(OH)D3, 22(OH)D3, 20,23(OH)(2)D3, 20,22(OH)(2)D3 and 17,20,23(OH)(3)D3. Some of these metabolites are hydroxylated by CYP27B1 at C1α, by CYP24A1 at C24 and C25, and by CYP27A1 at C25 and C26. The products of these pathways are biologically active. In the epidermis and/or serum or adrenals we detected 20(OH)D3, 22(OH)D3, 20,22(OH)(2)D3, 20,23(OH)(2)D3, 17,20,23(OH)(3)D3, 1,20(OH)(2)D3, 1,20,23(OH)(3)D3, 1,20,22(OH)(3)D3, 20,24(OH)(2)D3, 1,20,24(OH)(3)D3, 20,25(OH)(2)D3, 1,20,25(OH)(3)D3, 20,26(OH)(2)D3 and 1,20,26(OH)(3)D3. 20(OH)D3 and 20,23(OH)(2)D3 are non-calcemic, while the addition of an OH at C1α confers some calcemic activity. Molecular modeling and functional assays show that the major products of the pathway can act as “biased” agonists for the VDR with high docking scores to the ligand binding domain (LBD), but lower than that of 1,25(OH)(2)D3. Importantly, cell based functional receptor studies and molecular modeling have identified the novel secosteroids as inverse agonists of both RORα and RORγ receptors. Specifically, they have high docking scores using crystal structures of RORα and RORγ LBDs. Furthermore, 20(OH)D3 and 20,23(OH)(2)D3 have been tested in cell model that expresses a Tet-on RORα or RORγ vector and a RORE-LUC reporter (ROR-responsive element), and in a mammalian 2-hybrid model that test interactions between an LBD-interacting LXXLL-peptide and the LBD of RORα/γ. These assays demonstrated that the novel secosteroids have ROR-antagonist activities that were further confirmed by the inhibition of IL17 promoter activity in cells overexpressing RORα/γ. In conclusion, endogenously produced novel D3 hydroxy-derivatives can act both as “biased” agonists of the VDR and/or inverse agonists of RORα/γ. We suggest that the identification of large number of endogenously produced alternative hydroxy-metabolites of D3 that are biologically active, and of possible alternative receptors, may offer an explanation for the pleiotropic and diverse activities of vitamin D, previously assigned solely to 1,25(OH)(2)D3 and VDR

Large-scale Molecular Gas Distribution in the M17 Cloud Complex: Dense Gas Conditions of Massive Star Formation?

The non-uniform distribution of gas and protostars in molecular clouds is caused by combinations of various physical processes that are difficult to separate. We explore this non-uniform distribution in the M17 molecular cloud complex that hosts massive star formation activity using the 12CO (J = 1–0) and 13CO (J = 1–0) emission lines obtained with the Nobeyama 45 m telescope. Differences in clump properties such as mass, size, and gravitational boundedness reflect the different evolutionary stages of the M17-H ii and M17-IRDC clouds. Clumps in the M17-H ii cloud are denser, more compact, and more gravitationally bound than those in M17-IRDC. While M17-H ii hosts a large fraction of very dense gas (27%) that has a column density larger than the threshold of ~1 g cm−2 theoretically predicted for massive star formation, this very dense gas is deficient in M17-IRDC (0.46%). Our HCO+ (J = 1–0) and HCN (J = 1–0) observations with the Taeduk Radio Astronomy Observatory 14 m telescope trace all gas with a column density higher than 3 × 1022 cm−2, confirming the deficiency of high-density (≳105 cm−3) gas in M17-IRDC. Although M17-IRDC is massive enough to potentially form massive stars, its deficiency of very dense gas and gravitationally bound clumps can explain the current lack of massive star formation

Nova V4743 Sagittarii 2002: An Intermediate Polar Candidate

We present the results of 11 nights of CCD unfiltered photometry of V4743 Sgr (Nova Sgr 2002 # 3) from 2003 and 2005. We find two periods of 0.2799 d ~ 6.7 h and 0.01642 d ~ 24 min in the 2005 data. The long period is also present in the 2003 data, but only weak evidence of the shorter period is found in this year. The 24-min period is somewhat longer than the 22-min period, which was detected from X-ray observations. We suggest that the 6.7-h periodicity represents the orbital period of the underlying binary system and that the 24-min period is the beat periodicity between the orbital period and the X-ray period, which is presumably the spin period of the white dwarf. Thus, V4743 Sgr should be classified as an intermediate polar (DQ Her star). About six months after the nova outburst, the optical light curve of V4743 Sgr seemed to show quasi-periodic oscillations, which are typical of the transient phase in classical nova. Therefore, our results support the previous suggestion that the trans ition phase in novae may be related to intermediate polars.Comment: 7 pages, 6 figures, AJ accepte

Astrophysical Fluids of Novae: High Resolution Pre-decay X-ray spectrum of V4743 Sagittarii

Eight X-ray observations of V4743 Sgr (2002), observed with Chandra and XMM-Newton are presented. The nova turned off some time between days 301.9 and 371, and the X-ray flux subsequently decreased from day 301.9 to 526 following an exponential decline time scale of $(96 \pm 3)$ days. We use the absorption lines present in the SSS spectrum for diagnostic purposes, and characterize the physics and the dynamics of the expanding atmosphere during the explosion of the nova. The information extracted from this first stage is then used as input for computing full photoionization models of the ejecta in V4743 Sgr. The SSS spectrum is modeled with a simple black-body and multiplicative Gaussian lines, which provides us of a general kinematical picture of the system, before it decays to its faint phase (Ness et al. 2003). In the grating spectra taken between days 180.4 and 370, we can resolve the line profiles of absorption lines arising from H-like and He-like C, N, and O, including transitions involving higher principal quantum numbers. Except for a few interstellar lines, all lines are significantly blue-shifted, yielding velocities between 1000 and 6000 km/s which implies an ongoing mass loss. It is shown that significant expansion and mass loss occur during this phase of the explosion, at a rate $\dot{M} \approx (3-5) \times 10^{-4} ~ (\frac{L}{L_{38}}) ~ M_{\odot}/yr$. Our measurements show that the efficiency of the amount of energy used for the motion of the ejecta, defined as the ratio between the kinetic luminosity $L_{\rm kin}$ and the radiated luminosity $L_{\rm rad}$, is of the order of one.Comment: 25 pages, 9 figures. Accepted in book: Recent Advances in Fluid Dynamics with Environmental Applications, pp.365-39

Mesoscopic Fano Effect in a Quantum Dot Embedded in an Aharonov-Bohm Ring

The Fano effect, which occurs through the quantum-mechanical cooperation between resonance and interference, can be observed in electron transport through a hybrid system of a quantum dot and an Aharonov-Bohm ring. While a clear correlation appears between the height of the Coulomb peak and the real asymmetric parameter $q$ for the corresponding Fano lineshape, we need to introduce a complex $q$ to describe the variation of the lineshape by the magnetic and electrostatic fields. The present analysis demonstrates that the Fano effect with complex asymmetric parameters provides a good probe to detect a quantum-mechanical phase of traversing electrons.Comment: REVTEX, 9 pages including 8 figure