Ameloblastin induces tumor suppressive phenotype and enhances chemosensitivity to doxorubicin via Src-Stat3 inactivation in osteosarcoma

Ameloblastin (AMBN), the most abundant non-amelogenin enamel matrix protein, plays a role in ameloblast differentiation. Previously, we found that AMBN promoted osteogenic differentiation via the interaction between CD63 and integrin β1, leading to the inactivation of Src; however, how AMBN affects the malignant behavior of osteosarcoma is still unclear. Osteosarcoma affects the bone and is associated with poor prognosis because of the high rate of pulmonary metastases and drug resistance. Here we demonstrated that stable overexpression of AMBN induced apoptosis and suppressed colony formation and cell migration via the inactivation of Src-Stat3 pathway in human osteosarcoma cells. Moreover, AMBN induced chemosensitivity to doxorubicin. Thus, AMBN induced a tumor suppressive phenotype and chemosensitivity to doxorubicin via the AMBN-Src-Stat3 axis in osteosarcoma. Indeed, immunohistochemical expression of AMBN was significantly correlated with better outcome of osteosarcoma patients. Our findings suggest that AMBN can be a new prognostic marker and therapeutic target for osteosarcoma combined with conventional doxorubicin treatment

Preferred Pace Tapping Activates the Frontal Lobe

Background and aims : The purpose of the present study was to compare the activity of the frontal lobe when performing tasks at two paces by functional near-infrared spectroscopy(fNIRS). M ethods : Fifty healthy students from A University participated in the study. Oxygenated hemoglobin (oxy-Hb) concentrations (Δ［oxy-Hb］) were monitored during 30s finger-tapping with the non-dominant hand. There were two paces for finger-tapping : one pace the subject decided by themselves and the other pace given by metronome. The subjects answered a questionnaire after fNIRS measurement. Results : Performing tasks at Preferred Pace (PP) activates the frontal lobe of the orbito-frontal cortex area, and it tends to induce a feeling of difficulty,especially in females. Conclusions : Performing repetitive tasks at PP activates the frontal lobe. This finding will enable occupational therapists to select the subject’ s optimal pace according to the degree of attentional dysfunction

cancer (PreMBC): results of Japan Clinical Oncology Group Study 9404

Tamoxifen plus tegafur‑uracil (TUFT) versus tamoxife