Clinical attachment loss and molecular profile of inflamed sites before treatment

Objective: To monitor early periodontal disease progression and to investigate clinical and molecular profile of inflamed sites by means of crevicular fluid and gingival biopsy analysis. Methodology: Eighty-one samples of twenty-seven periodontitis subjects and periodontally healthy individuals were collected for the study. Measurements of clinical parameters were recorded at day −15, baseline and 2 months after basic periodontal treatment aiming at monitoring early variations ofthe clinical attachment level. Saliva, crevicular fluid and gingival biopsies were harvested from clinically inflamed and non-inflamed sites from periodontal patients and from control sites of healthy patients for the assessment of IL-10, MMP-8, VEGF, RANKL, OPG and TGF-β1 protein and gene expression levels. Results:Baseline IL-10 protein levels from inflamed sites were higher in comparison to both non-inflamed and control sites (p<0.05). Higher expression of mRNA for IL-10, RANK-L, OPG, e TGF-β1 were also observed in inflamed sites at day −15 prior treatment (p<0.05). After the periodontal treatment and the resolution of inflammation, seventeen percent of evaluated sites still showed clinically detectable attachment loss without significant differences in the molecular profile. Conclusions: Clinical attachment loss is a negative event that may occur even after successful basic periodontal therapy, but it is small and limited to a small percentage of sites. Elevated inflammation markers of inflamed sites from disease patients reduced to the mean levels of those observed in healthy subjects after successful basic periodontal therapy. Significantly elevated both gene and protein levels of IL-10 in inflamed sites prior treatment confirms its modulatory role in the disease status

Periodontal status of an indigenous population at the Xingu reserve

Aim : To describe the prevalence of periodontal disease in the indigenous population of the Middle and Lower Xingu compared to the non-indigenous Brazilian population. Methods: The evaluated indigenous population of the Xingu Reserve had oral and dental examinations performed by calibrated examiners assisted by Oral Health Indigenous Agents. From a sample of 2,299 indigenous subjects, epidemiological investigations were conducted in 1,911 individuals, using the methodology recommended by the World Health Organization. Comparative periodontal data from the non-indigenous population were obtained from the Brazilian Ministry of Health′s national epidemiological survey on oral health conditions ("SB Brasil" project). The periodontal data of 508 indigenous individuals were presented by age intervals of 15-19 (n=219), 35-44 (n=128) and 65-74 (n=161) years. Results: In the non-indigenous population, the periodontally healthy individuals were 46.2%, 21.9% and 7.9% for each age group, respectively, and in the Xingu population they were 28.76%, 3.12% and 0% for each age group, respectively. The most frequent finding in the Xingu population was the presence of calculus in 62.55% of younger people, 82.03% of adults and 45.45% of the elderly. The analysis by sextants demonstrated the presence of calculus in 25.04%, 44.79% and 18.18% for young, adults and elderly respectively. Conclusions : Despite the higher prevalence of calculus, in all age groups of the indigenous population, tooth loss does not seem to follow the same pattern observed in the non-indigenous Brazilian population, suggesting differences in susceptibility, habits or conditions

Periodontal status of an indigenous population at the Xingu Reserve

describe the prevalence of periodontal disease in the indigenous population of the Middle and Lower Xingu compared to the non-indigenous Brazilian population. Methods: The evaluated indigenous population of the Xingu Reserve had oral and dental examinations performed by calibrated examiners assisted by Oral Health Indigenous Agents. From a sample of 2,299 indigenous subjects, epidemiological investigations were conducted in 1,911 individuals, using the methodology recommended by the World Health Organization. Comparative periodontal data from the nonindigenous population were obtained from the Brazilian Ministry of Healths national epidemiological survey on oral health conditions (SB Brasil project). The periodontal data of 508 indigenous individuals were presented by age intervals of 15-19 (n=219), 35-44 (n=128) and 65-74 (n=161) years. Results: In the non-indigenous population, the periodontally healthy individuals were 46.2%, 21.9% and 7.9% for each age group, respectively, and in the Xingu population they were 28.76%, 3.12% and 0% for each age group, respectively. The most frequent finding in the Xingu population was the presence of calculus in 62.55% of younger people, 82.03% of adults and 45.45% of the elderly. The analysis by sextants demonstrated the presence of calculus in 25.04%, 44.79% and 18.18% for young, adults and elderly respectively. Conclusions: Despite the higher prevalence of calculus, in all age groups of the indigenous population, tooth loss does not seem to follow the same pattern observed in the non-indigenous Brazilian population, suggesting differences in susceptibility, habits or conditions