Çeşitli Geçici Dolguların Standardize Edilmiş Endodontik Giriş Kavitelerinde Koronal Mikrosızıntısı

Amaç: Bu çalışmanın amacı, çekilmiş dişlerdeki endodontik giriş kavitelerinde çeşitli geçici dolguların (GD) koronal mikrosızıntını metilen mavisi boya penetrasyon testi ile karşılaştırmaktır. Gereç ve Yöntem: Yüz adet çekilmiş premolar dişe standart giriş kaviteleri hazırlanmıştır. Dişler rastgele 10’ar diş içeren 9 gruba ayrılmış, kalan dişler pozitif ve negative control olarak kullanılmıştır. Deney gruplarındaki kaviteler 4 mm derinliğinde; Cavit G, Coltosol F, BMS, Fermin, Ketac Molar Easymix, Clip, ProFill, DuoTEMP, veya Triklosanlı TempBond Clear GD ile üretici firma önerilerine göre doldurulmuştur. Dişler 500 kez (5-55°C) termosiklusa tabii tutulduktan sonra, metilen mavisi boya penetrasyon testi ile dolguların mikrosızıntıları ölçülmüştür. Dişler ortadan ikiye ayrılmış ve boya penetrasyonunun en derin olduğu değer kaydedilmiştir. Koronal mikrosızıntı bir stereomikroskop ile değerlendirilmiştir. Veriler KruskalWallis ve Bonferroni düzeltmeli Mann-Whitney U testi ile değerlendirilmiştir (p ? 0,05). Bulgular: Pozitif kontroller tam boya penetrasyonu gösterirken, negative kontrollerde hiç boya penetrasyonu görülmemiştir. Pro-Fil geçici dolgu, DuoTEMP, BMS, Coltosol, Cavit-G’den anlamı düzeyde daha az koronal örtme sağlamasına rağmen (p? 0.05), diğer gruplar arasında anlamlı bir fark bulunmamıştır (p> 0.05). Bununla birlikte, en az mikrosızıntıyı Ketac Molar Easymix göstermiştir.(p? 0.05). Sonuç: Tüm GDlar mikrosızıntı göstermiştir. Bu çalışmanın sonuçları Ketac Molar Easymix’in GD olarak kullanımının uygun olmadığını, diğer materyallerin değişik sızıntı değerleri göz önünde bulundurularak kullanılabileceklerini göstermiştir.Background and Aim: The aim of this study was to compare the coronal microleakage of various temporary fillings (TFs) in endodontic access cavities in extracted human teeth using a methylene blue dye penetration test. Materials and Methods: Standardized access cavities were prepared in 100 extracted human premolars. The teeth were then randomly divided into 9 groups of 10 teeth, with the remaining teeth serving as positive and negative controls. The cavities in the experimental groups were filled with 4 mm of Cavit G, Coltosol F, BMS, Fermin, Ketac Molar Easymix, Clip, ProFill, DuoTEMP, or TempBond Clear with Triclosan TFs according to the manufacturer’s instructions. After thermocycling for 500 cycles (5-55ºC), microleakage was measured by using a methylene blue dye penetration test. The teeth were sectioned, and the greatest depth of dye penetration was recorded. Coronal microleakage was evaluated with a stereomicroscope. Data was analyzed with Kruskal-Wallis and Mann-Whitney U tests with Bonferroni correction (p≤ 0.05). Results: Positive controls displayed complete dye penetration, and negative controls showed no dye penetration. Whereas Pro-Fill led to a statistically significantly lesser coronal sealing ability than DuoTEMP, BMS, Coltosol, Cavit-G (p≤ 0.05), there was no significant difference between the other groups (p>0.05). However, Ketac Molar Easymix showed lowest leakage (p≤ 0.05). Conclusion: All TFs displayed some degree of leakage. The results of this study indicate that Ketac Molar Easymix is not suitable material for temporary filling. Other materials can be used, but should be considered their variable leakage rates

Cytotoxicity Analysis of Strontium Ranelate on Cultured Human Periodontal Ligament Fibroblasts: A Preliminary Report

Background/Purpose: The aim of this study was to analyze the cytotoxicity of strontium ranelate (SR) on human periodontal ligament fibroblasts (PDL cells) in vitro. Methods: PDL cells were obtained from healthy human third molars and cultured in Dulbecco's Modified Eagle's Medium. The experimental groups were: G1, cultures treated with fresh medium (control); and G2, G3, G4 and G5: treated with SR at 20, 10, 5 and 2.5 mg/mL, respectively. The experimental times were 1, 6, 12 and 24 hours (short-term) for viability, and 2, 4, 6 and 8 days (long-term) for cell survival. The cells were counted using a hemocytometer. Data were then analyzed by one-way ANOVA and Tukey's tests (p < 0.05). Results: Cultures treated with the highest SR concentrations (G2 and G3) had significantly lower cell viability and cell numbers (p < 0.05) than those in G1, G4 and G5. SR at 2.5 mg/mL was non-cytotoxic to PDL cells. Conclusion: SR was non-toxic at appropriate concentrations. Preclinical tests are needed to further assess its safety and effectiveness for tooth resorption prior to clinical use

Bond Strength of Calcium Silicate-Based Sealers to Dentine Dried with Different Techniques

Objective To assess the influence of various canal-drying techniques on the push-out bond strength between radicular dentine and both MTA Fillapex and iRoot SP sealers. Materials and Methods Eighty extracted single-root teeth were instrumented with ProTaper Universal rotary instruments to a size F4. The teeth were randomly assigned to 1 of 4 groups as follows: group 1, excess fluid in the canals was removed using a Luer vacuum adapter; group 2, the canals were dried with a single paper point; group 3, the canals were dried with 3–5 paper points, and group 4, the canals were rinsed with 95% ethanol and then dried with 3–5 paper points. In each group, the specimens were subgrouped according to root canal sealer into either subgroup A (MTA Fillapex) or subgroup B (iRoot SP sealer). Horizontal sections (thickness 1 ± 0.1 mm) were obtained from each specimen and a push-out test was performed using a universal testing machine at a crosshead speed of 1 mm/min on root slices. Statistical analysis was done using the Kruskal-Wallis test and Dunn's test for pair-wise comparisons. p 0.05). Conclusions The canal-drying technique influenced the adhesive bond strength between calcium silicate-based root canal sealers and the root canal wall.PubMedWoSScopu

Neurotoxicity of various root canal sealers on rat sciatic nerve: an electrophysiologic and histopathologic study

Tasdemir, Tamer/0000-0001-8512-5280; Er, Kursat/0000-0002-0667-4909; Yildirim, Mehmet/0000-0003-1798-5478WOS: 000362283100040PubMed: 25773448Objectives the aim of this study was to compare the neurotoxicity of various root canal sealers on rat sciatic nerve by electrophysiologic and histopathologic analyses. Materials and methods A total of 40 male rats were randomly divided into five groups: Control, AH Plus, GuttaFlow, Sealapex and Smartpastebio. Sciatic nerves of the rats were uncovered using the surgical procedures, and the prepared sealers were then applied on nerves with a polyethylene tube vehicle for 15 days. Nerve potentials were recorded at initial exposure, 5, 30 and 120 min (early phase), and 15 days (late phase) by an electrophysiologic analysis system for all groups. the obtained measurements were then used to calculate the nerve conduction velocities (NCV). Subsequently, all rats were sacrificed, and their sciatic nerves were removed for histopathologic analysis. Statistical analysis was performed using the Kruskal-Wallis and Mann-Whitney U tests for intergroup variables and the Friedman and Wilcoxon test for intragroup variables. Statistical significance was set at P < 0.05. Results There was no significant difference between early and late phase results in the control group. This group showed little or no lasting damage to nerve tissue. All sealers decreased the NCV in the early phase time periods, but this decrease was only statistically significant in the AH Plus group at 120-min time period (P < 0.0125). During the late phase, the AH Plus and GuttaFlow groups almost reached initial NCV values, and it was lower than the initial values in the Sealapex and Smartpastebio groups. However, this decrease was not statistically significant. When intergroup comparisons were performed, statistically significant differences occurred at 30 min in the Sealapex group and 120 min in the AH Plus group compared with the control group (P < 0.0125). All sealers induced neurotoxicity as a result of degenerative and inflammatory responses of nerve tissue in histologic analysis. Histologic analysis revealed Sealapex and GuttaFlow to be the most and least neurotoxic, respectively. Conclusions All tested root canal sealers exhibited a variable degree of neurotoxicity depending on their chemical compositions