5 research outputs found
Practical, Microfabrication-Free Device for Single-Cell Isolation
- Author
- Publication venue
- Public Library of Science
- Publication date
- 01/01/2009
- Field of study
Microfabricated devices have great potential in cell-level studies, but are not easily accessible for the broad biology community. This paper introduces the Microscale Oil-Covered Cell Array (MOCCA) as a low-cost device for high throughput single-cell analysis that can be easily produced by researchers without microengineering knowledge. Instead of using microfabricated structures to capture cells, MOCCA isolates cells in discrete aqueous droplets that are separated by oil on patterned hydrophilic areas across a relatively more hydrophobic substrate. The number of randomly seeded Escherichia coli bacteria in each discrete droplet approaches single-cell levels. The cell distribution on MOCCA is well-fit with Poisson distribution. In this pioneer study, we created an array of 900-picoliter droplets. The total time needed to seed cells in ∼3000 droplets was less than 10 minutes. Compared to traditional microfabrication techniques, MOCCA dramatically lowers the cost of microscale cell arrays, yet enhances the fabrication and operational efficiency for single-cell analysis
Real-time single-cell imaging of protein secretion
- Author
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- Field of study
Measuring gene expression in single bacterial cells: recent advances in methods and micro-devices
- Author
- Altindal T
- Amann RI
- Anis Y
- Anis Y
- Aoi Y
- Arumugam PU
- Banaeiyan AA
- Banerjee B
- Baptista M
- Beer NR
- Beta C
- Boedicker J
- Brady G
- Button DK
- Carmon G
- Colman-Lerner A
- Deirdre R. Meldrum
- Dupont CL
- Fritzsch FS
- Fuchs P
- Furutani S
- Gao W
- Gao W
- Goetz JJ
- Grate D
- Grünberger A
- Heinemann M
- Hodson RE
- Ibrahim SF
- Jiangxin Wang
- Kang Y
- Kelly CD
- Kerppola TK
- Kuang Y
- Kurimoto K
- Kühn S
- Lange M
- Le TT
- Leung K
- Lin L.-I.
- Lindqvist N
- Lutz BR
- Magliery TJ
- Maloney PC
- Mckenna BK
- Mirsaidov U
- Molter TW
- Molter TW
- Morrison LE
- Neuman KC
- Ottesen EA
- Pamp SJ
- Peitz I
- Pijper A
- Pohl HA
- Rasmussen MB
- Roeder B
- Roman GT
- Sando S
- Santangelo PJ
- Schmid A
- Schneider J
- Schut F
- Shi X
- Shi X
- Shih-Hui Chao
- Singh R
- Spits C
- Strovas TJ
- Strovas TJ
- Tang F
- Taniguchi K
- Tanyeri M
- Tanyeri M
- Teramoto J
- Tischler J
- Tolker-Nielsen T
- Um E
- Valencia-Burton M
- Wacker MJ
- Wang D
- Weimin Gao
- Weiwen Zhang
- White AK
- Wijgerde M
- Wood TH
- Woyke T
- Xu Shi
- Yang SW
- Yi WC
- Zhang H
- Zhu H
- Publication venue
- 'Informa UK Limited'
- Publication date
- Field of study
Polarization-sensitive THz-TDS and its Application to Anisotropy Sensing
- Author
- A Hussain
- A Mitsuishi
- A Rubano
- A Wojdyla
- AJL Adam
- B Scherger
- C Fattinger
- C-F Hsieh
- CJ Summers
- D Grischkowsky
- D Molter
- DK George
- E Castro-Camus
- ED Palik
- F Gervais
- F Miyamaru
- F Miyamaru
- F Miyamaru
- F Miyamaru
- FJ Abajo de
- FJ Garcia-Vidal
- G Dodel
- G Dresselhaus
- GP Bryan-Brown
- H Makabe
- H Sumikura
- I Yamada
- J Kircher
- J Kyoung
- J Masson
- JB Pendry
- JH Boer
- K Kozuki
- K Takano
- K-L Barth
- L Ren
- L Zhang
- LY Deng
- M Hangyo
- M Hangyo
- M Hasselbeck
- M Kuwata-Gonokami
- M Neshat
- M Shalaby
- Masahiko Tani
- Masanori Hangyo
- N Kanda
- N Matsumoto
- N Matsumoto
- N Valk van der
- N Yasumatsu
- N Yasumatsu
- O Morikawa
- PCM Planken
- Q Chen
- Q Chen
- R Shimano
- R Shimano
- R Zhang
- RI Hunter
- RL Johnson
- S Kojima
- S Nashima
- SC Saha
- T Hofmann
- T Kondo
- T Nagashima
- T-I Jeon
- Takeshi Nagashima
- TW Ebbesen
- W Spitzer
- XG Peralta
- Y Ikebe
- Y Pastol
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- Field of study
Assessing written work by determining competence to achieve the module-specific learning outcomes.
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- A Aznar
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- A Bayramian
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- A Bloom
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- X Liu
- X Mateos
- X Mateos
- X Mateos
- X Mateos
- X Yang
- X Yu
- X-L Zhu
- X-L Zhu
- XS Zhu
- XS Zhu
- XS Zhu
- XS Zhu
- XS Zhu
- XY Zhang
- Y Akahane
- Y Arakawa
- Y Bo
- Y Chen
- Y Cheng
- Y Cheng
- Y Cheng
- Y Cui
- Y Ding
- Y Ishida
- Y Jeong
- Y Jeong
- Y Jeong
- Y Kimura
- Y Kotaki
- Y Lu
- Y Millerioux
- Y Nabekawa
- Y Nabekawa
- Y Nagata
- Y Nagata
- Y Nakano
- Y Nishida
- Y Ohiso
- Y Oki
- Y Oki
- Y Oki
- Y Pang
- Y Qi
- Y Qi
- Y Segawa
- Y Sorek
- Y Tamaki
- Y Tamaki
- Y Tamaki
- Y Tamaki
- Y Tamaki
- Y Tanabe
- Y Tanabe
- Y Tanabe
- Y Tohmori
- Y Wang
- Y Wang
- Y Wang
- Y Yamada
- Y Yanning
- Y Zhao
- Y Zhao
- Y.K. Chembo
- YA Afanasiev
- YD Huang
- YD West
- YD West
- YD Zavartsev
- YD Zavartsev
- YD Zavartsev
- YE Romanyuk
- YF Qi
- YH Chen
- YH Tsang
- YH Tsang
- YH Xue
- YH Xue
- YH Xue
- YJ Chen
- YK Bu
- YK Hsu
- YK Kim
- YK Voronko
- YM Cheung
- YM Wang
- YP Raizer
- YP Tong
- YP Zhang
- YP Zhang
- YR Shen
- YS Derbenev
- YS Vagin
- YT Tzong
- YU Jeong
- YV Baklanov
- YX Fan
- YX Fan
- YY Kalisky
- YZ Wang
- Z Cheng
- Z Liu
- Z Liu
- Z Liu
- Z Luo
- Z Zhang
- Z Zhang
- ZH Wang
- ZI Alferov
- ZJ Kiss
- ZJ Kiss
- ZT Azamatov
- Publication venue
- Publication date
- 01/01/2012
- Field of study
This chapter describes lasers and other sources of coherent light that operate in a wide wavelength range. First, the general principles for the generation of coherent continuous-wave and pulsed radiation are treated including the interaction of radiation with matter, the properties of optical resonators and their modes as well as such processes as Q-switching and mode-locking. The general introduction is followed by sections on numerous types of lasers, the emphasis being on todayʼs most important sources of coherent light, in particular on solid-state lasers and several types of gas lasers. An important part of the chapter is devoted to the generation of coherent radiation by nonlinear processes with optical parametric oscillators, difference- and sum-frequency generation, and high-order harmonics. Radiation in the extended ultraviolet (EUV) and x-ray ranges can be generated by free electron lasers (FEL) and advanced x-ray sources. Ultrahigh light intensities up to 1021 W/cm2 open the door to studies of relativistic laser–matter interaction and laser particle acceleration. The chapter closes with a section on laser stabilization