Effect of fulvic acids on lead-induced oxidative stress to metal sensitive Vicia faba L. plant

Lead (Pb) is a ubiquitous environmental pollutant capable to induce various morphological, physiological, and biochemical functions in plants. Only few publications focus on the influence of Pb speciation both on its phytoavailability and phytotoxicity. Therefore, Pb toxicity (in terms of lipid peroxidation, hydrogen peroxide induction, and photosynthetic pigments contents) was studied in Vicia faba plants in relation with Pb uptake and speciation. V. faba seedlings were exposed to Pb supplied as Pb(NO3)2 or complexed by two fulvic acids (FAs), i.e. Suwannee River fulvic acid (SRFA) and Elliott Soil fulvic acid (ESFA), for 1, 12, and 24 h under controlled hydroponic conditions. For both FAs, Pb uptake and translocation by Vicia faba increased at low level (5 mg l−1), whereas decreased at high level of application (25 mg l−1). Despite the increased Pb uptake with FAs at low concentrations, there was no influence on the Pb toxicity to the plants. However, at high concentrations, FAs reduced Pb toxicity by reducing its uptake. These results highlighted the role of the dilution factor for FAs reactivity in relation with structure; SRFA was more effective than ESFA in reducing Pb uptake and alleviating Pb toxicity to V. faba due to comparatively strong binding affinity for the heavy metal

Metal uptake and nanoparticle synthesis in hairy root cultures

Hairy roots are a convenient experimental tool for investigating the interactions between plant cells and metal ions. Hairy roots of species capable of hyperaccumulating Cd and Ni have been applied to investigate heavy metal tolerance in plants; hairy roots of nonhyperaccumulator species have also been employed in metal uptake studies. Furnace treatment of hairy root biomass containing high concentrations of Ni has been used to generate Ni-rich bio-ore suitable for metal recovery in phytomining applications. Hairy roots also have potential for biological synthesis of quantum dot nanocrystals. As plant cells intrinsically provide the confined spaces needed to limit the size of nanocrystals, hairy roots cultured in bioreactors under controlled conditions are a promising vehicle for the manufacture of peptide-capped semiconductor quantum dots