Mobility Measurements Probe Conformational Changes in Membrane Proteins due to Tension

The function of membrane-embedded proteins such as ion channels depends crucially on their conformation. We demonstrate how conformational changes in asymmetric membrane proteins may be inferred from measurements of their diffusion. Such proteins cause local deformations in the membrane, which induce an extra hydrodynamic drag on the protein. Using membrane tension to control the magnitude of the deformations and hence the drag, measurements of diffusivity can be used to infer--- via an elastic model of the protein--- how conformation is changed by tension. Motivated by recent experimental results [Quemeneur et al., Proc. Natl. Acad. Sci. USA, 111 5083 (2014)] we focus on KvAP, a voltage-gated potassium channel. The conformation of KvAP is found to change considerably due to tension, with its `walls', where the protein meets the membrane, undergoing significant angular strains. The torsional stiffness is determined to be 26.8 kT at room temperature. This has implications for both the structure and function of such proteins in the environment of a tension-bearing membrane.Comment: Manuscript: 4 pages, 4 figures. Supplementary Material: 8 pages, 1 figur

Recycling controls membrane domains

We study the coarsening of strongly microphase separated membrane domains in the presence of recycling of material. We study the dynamics of the domain size distribution under both scale-free and size-dependent recycling. Closed form solutions to the steady state distributions and its associated central moments are obtained in both cases. Moreover, for the size-independent case, the~time evolution of the moments is analytically calculated, which provide us with exact results for their corresponding relaxation times. Since these moments and relaxation times are measurable quantities, the biophysically significant free parameters in our model may be determined by comparison with experimental data.Comment: 5 pages, 4 figure

Density regulation in strictly metric-free swarms

There is now experimental evidence that nearest-neighbour interactions in flocks of birds are metric free, i.e. they have no characteristic interaction length scale. However, models that involve interactions between neighbours that are assigned topologically are naturally invariant under spatial expansion, supporting a continuous reduction in density towards zero, unless additional cohesive interactions are introduced or the density is artificially controlled, e.g. via a finite system size. We propose a solution that involves a metric-free motional bias on those individuals that are topologically identified to be on an edge of the swarm. This model has only two primary control parameters, one controlling the relative strength of stochastic noise to the degree of co-alignment and another controlling the degree of the motional bias for those on the edge, relative to the tendency to co-align. We find a novel power-law scaling of the real-space density with the number of individuals N as well as a familiar order-to-disorder transition

A Machian Model of Dark Energy

Einstein believed that Mach's principle should play a major role in finding a meaningful spacetime geometry, though it was discovered later that his field equations gave some solutions which were not Machian. It is shown, in this essay, that the kinematical $\Lambda$ models, which are invoked to solve the cosmological constant problem, are in fact consistent with Mach's ideas. One particular model in this category is described which results from the microstructure of spacetime and seems to explain the current observations successfully and also has some benefits over the conventional models. This forces one to think whether the Mach's ideas and the cosmological constant are interrelated in some way.Comment: Received an Honorable mention in the Essay Contest-2002 sponsored by the Gravity Research Foundation; A paragraph added on how the model can explain the CMB anisotropy observations; To appear in the Classical and Quantum Gravit

Referencing within code in software engineering education

Traditionally, computer sciences courses will assess software code. It is common and accepted good practice (as in written reports) to reference other sources of appropriate material. However there appears to be no explicit method, recommendation or advice available to computer science tutors and students on a referencing approach! This paper aims to stimulate discussion from peers involved in software engineering education. By discussing the apparent lack of ‘referencing within code’ advice to students and proposing suggestions for appropriate solutions. This will be based on the authors’ experience of assessing code and the current advice given to their students

Expectations for the Difference Between Local and Global Measurements of the Hubble Constant

There are irreducible differences between the Hubble constant measured locally and the global value. They are due to density perturbations and finite sample volume (cosmic variance) and finite number of objects in the sample (sampling variance). We quantify these differences for a suite of COBE-normalized CDM models that are consistent with the observed large-scale structure. For small samples of objects that only extend out to 10,000 km/sec, the variance can approach 4%. For the largest samples of Type Ia supernovae (SNeIa), which include about 40 objects and extend out to almost 40,000 km/sec, the variance is 1-2% and is dominated by sampling variance. Sampling and cosmic variance may be an important consideration in comparing local determinations of the Hubble constant with precision determinations of the global value that will be made from high-resolution maps of CBR anisotropy.Comment: 10 pages, Latex, 2 figures, version accepted for Ap.

Use of an audio-paced incremental swimming test in young national-level swimmers

Purpose:To evaluate the reliability and sensitivity to training of an audio-paced incremental swimming test.Methods:Eight young national-level male swimmers (age 15 ± 1 year) performed a 7 × 200-m incremental swimming test (velocities 1.19, 1.24, 1.28, 1.33, 1.39, and 1.45 m/s and maximal sprint pace) using an audio-pacing device. The same test was performed 4 times by each participant, 1 wk apart to assess reliability (WK1, WK2) and after 9 and 20 wk of training (WK9, WK20). Blood lactate concentration ([La−]) and heart rate (HR) were recorded after each stage. Outcome measures were the velocity (v) and HR at lactate markers of 2 mM, 4 mM, and Δ1 mM.Results:Velocities at the lactate markers proved to be more reliable than HR, with typical errors ranging from 0.66% to 2.30% and 1.28% to 4.50%, respectively (shifts in mean ranged –0.91% to 0.73% and –0.84% to 1.79%, respectively). Across WK1, WK9, and WK20 there were significant improvements in peak velocity (P &lt; .001) and each of the velocities associated with the lactate markers (P &lt; .05), whereas only HR at Δ1 mM improved (P &lt; .05).Conclusions:This article demonstrates that an audio-paced incremental swimming test is reliable for use with junior swimmers and is sensitive to changes observed after training. The postswimming measurement of HR in the pool was comparatively less reliable.</jats:sec

Distributed simulation and the grid: Position statements

The Grid provides a new and unrivaled technology for large scale distributed simulation as it enables collaboration and the use of distributed computing resources. This panel paper presents the views of four researchers in the area of Distributed Simulation and the Grid. Together we try to identify the main research issues involved in applying Grid technology to distributed simulation and the key future challenges that need to be solved to achieve this goal. Such challenges include not only technical challenges, but also political ones such as management methodology for the Grid and the development of standards. The benefits of the Grid to end-user simulation modelers also are discussed