Long-term Performance and Community Analysis of Spirodela Polyrrhiza-bacteria Association Treating Phenol-contaminated Water

『大阪大学大学院工学研究科環境・エネルギー工学専攻生物圏環境工学領域 研究活動報告』, (2010.4.1～2011.3.31), pp.83-94, 大阪大学大学院工学研究科環境・エネルギー工学専攻環境資源・材料学講座生物圏環境工学領域, 2011.5 に掲

The Impacts of Albuminuria and Low eGFR on the Risk of Cardiovascular Death, All- cause Mortality, and Renal Events in Diabetic Patients: Meta-Analysis

13301甲第3978号博士（医学）金沢大学博士論文本文Full 以下に掲載：PLOS ONE 8(8) pp.e71810-e71810 2013. Library pf science. 共著者：Tadashi Toyama, Kengo Furuichi, Toshiharu Ninomiya, Miho Shimizu, Akinori Hara, Yasunori Iwata, Shuichi Kaneko, Takashi Wad

ACCELERATED PHENOL DEGRADATION BY BACTERIAL COMMUNITY IN THE RHIZOSPHERE OF SPIRODELA POLLYRHIZA

Joint Research on Environmental Science and Technology for the Eart

ACCELERATED AROMATIC COMPOUNDS DEGRADATION IN AQUATIC ENVIRONMENT BY USE OF INTERACTION BETWEEN SPIRODELA POLYRRHIZA AND BACTERIA IN ITS RHIZOSPHERE

Joint Research on Environmental Science and Technology for the Eart

Characterization of Novel 4-n-Butylphenol- Degrading Pseudomonas Veronii Strains Isolated from Rhizosphere of Giant Duckweed, Spirodela polyrrhiza

『大阪大学大学院工学研究科環境・エネルギー工学専攻生物圏環境工学領域 研究活動報告』, (2009.4.1～2010.3.31), pp.32-41, 大阪大学大学院工学研究科環境・エネルギー工学専攻環境資源・材料学講座生物圏環境工学領域, 2010.5 に掲

Isolation and characterization of tetrachloroethylene- and cis-1,2-dichloroethylene-dechlorinating propionibacteria

Two rapidly growing propionibacteria that could reductively dechlorinate tetrachloroethylene (PCE) and cis-1,2-dichloroethylene (cis-DCE) to ethylene were isolated from environmental sediments. Metabolic characterization and partial sequence analysis of their 16S rRNA genes showed that the new isolates, designated as strains Propionibacterium sp. HK-1 and Propionibacterium sp. HK-3, did not match any known PCE- or cis-DCE-degrading bacteria. Both strains dechlorinated relatively high concentrations of PCE (0.3 mM) and cis-DCE (0.52 mM) under anaerobic conditions without accumulating toxic intermediates during incubation. Cell-free extracts of both strains catalyzed PCE and cis-DCE dechlorination; degradation was accelerated by the addition of various electron donors. PCE dehalogenase from strain HK-1 was mediated by a corrinoid protein, since the dehalogenase was inactivated by propyl iodide only after reduction by titanium citrate. The amounts of chloride ions (0.094 and 0.103 mM) released after PCE (0.026 mM) and cis-DCE (0.05 mM) dehalogenation using the cell-free enzyme extracts of both strains, HK-1 and HK-3, were stoichiometrically similar (91 and 100%), indicating that PCE and cis-DCE were fully dechlorinated. Radiotracer studies with [1,2-¹⁴C] PCE and [1,2-¹⁴C] cis-DCE indicated that ethylene was the terminal product; partial conversion to ethylene was observed. Various chlorinated aliphatic compounds (PCE, trichloroethylene, cis-DCE, trans-1,2-dichloroethylene, 1,1-dichloroethylene, 1,1-dichloroethane, 1,2-dichloroethane, 1,2-dichloropropane, 1,1,2-trichloroethane, and vinyl chloride) were degraded by cell-free extracts of strain HK-1

Clinical impact of albuminuria in diabetic nephropathy

金沢大学医薬保健研究域医学系Patients suffering from diabetic nephropathy, resulting in end-stage renal failure, are increasing in number. The pathophysiology of diabetic nephropathy remains to be fully investigated. In the clinical setting, the presence of albuminuria/overt proteinuria and a low glomerular filtration rate may predict poor renal prognosis, but the prognosis of the normoalbuminuric renally insufficient diabetic patient remains controversial. In addition to the measurement of urinary albumin excretion, biomarker studies to detect diabetic nephropathy more specifically at the early stage have been performed worldwide. There is a growing body of evidence for remission and/or regression of diabetic nephropathy, which may be an indicator for cardiovascular and renal risk reduction. Deeper insights into the pathological characteristics as well as the clinical impact of albuminuria on renal and cardiovascular outcome are required. © 2011 Japanese Society of Nephrology

The Impacts of Albuminuria and Low eGFR on the Risk of Cardiovascular Death, All-Cause Mortality, and Renal Events in Diabetic Patients: Meta-Analysis

Background:Precise effects of albuminuria and low estimated glomerular filtration rate (eGFR) on cardiovascular mortality, all-cause mortality, and renal events in diabetic patients are uncertain.Materials and Methods:A systematic review was conducted of the literature through MEDLINE, EMBASE, and CINHAL from 1950 to December 2010. Cohort studies of diabetic patients providing adjusted relative risk (RR) of albuminuria and eGFR for risks of cardiovascular mortality, all-cause mortality, and renal events were selected. Two reviewers screened abstracts and full papers of each study using standardized protocol.Results:We identified 31 studies fulfilling the criteria from 6546 abstracts. With regard to the risk of cardiovascular mortality, microalbuminuria (RR 1.76, 95%CI 1.38-2.25) and macroalbuminuria (RR 2.96 95%CI 2.44-3.60) were significant risk factors compared to normoalbuminuria. The same trends were seen in microalbuminuria (RR 1.60, 95%CI 1.42-1.81), and macroalbuminuria (RR 2.64, 95%CI 2.13-3.27) for the risk of all-cause mortality, and also in microalbuminuria (RR 3.21, 95%CI 2.05-5.02) and macroalbuminuria (RR 11.63, 95%CI 5.68-23.83) for the risk of renal events. The magnitudes of relative risks associated with low eGFR along with albuminuria were almost equal to multiplying each risk rate of low eGFR and albuminuria. No significant factors were found by investigating potential sources of heterogeneity using subgroup analysis.Conclusions:High albuminuria and low eGFR are relevant risk factors in diabetic patients. Albuminuria and low eGFR may be independent of each other. To evaluate the effects of low eGFR, intervention, or race, appropriately designed studies are needed. © 2013 Toyama et al