Atomic Layer Deposition to Fine-Tune the Surface Properties and Diameters of Fabricated Nanopores

Atomic layer deposition of alumina enhanced the molecule sensing characteristics of fabricated nanopores by fine-tuning their surface properties, reducing 1/f noise, neutralizing surface charge to favor capture of DNA and other negative polyelectrolytes, and controlling the diameter and aspect ratio of the pores with near single Ångstrom precision. The control over the chemical and physical nature of the pore surface provided by atomic layer deposition produced a higher yield of functional nanopore detectors.Molecular and Cellular BiologyPhysicsChemistry and Chemical BiologyEngineering and Applied Science

Histochemical Characteristics of Tertiary Dentin Due to Calcium Hydroxide Paste in Rats

Calcium hydroxide is mainly used for dental pulp capping and it is thought that it induces hard tissue formation far better than other materials. Experimentally verifying this fact, Nishikawa et al revealed that bone-like dentin corresponding to tertiary dentin is rapidly formed when calcium hydroxide is applied directly to the pulp. Utilizing the same experimental system, histochemical study of the newly formed hard tissue (reparative dentin) was carried out and the results thereof were reported. Thick and irregular reparative dentin was formed in the pulp cavity and partial narrowing of the root canal was observed in m-CT. Histopathologically, the irregular reparative dentin increased its thickness obliterating the root canal having a diff erent Azan staining of aniline blue compared to primary dentin. Numerous cellular inclusion bodies were also trapped inside the thick dentin. Furthermore, with Schmorl’s thionine picric acid staining,thick reparative dentin was noted around the pulp cavity and dentin. The dentin was densely stained with picric acid with diff erent staining ability from the surrounding dentin. In addition, it was clearly confi rmed that many cells were trapped in reparative dentin. The results of the experiment suggest that the characteristics of the newly formed reparative dentin is comparable to tertiary dentin

Combined Laparoscopic and CT Monitoring of the Ice-Ball Margin during Cryoablation for Renal Cell Carcinoma Associated with von Hippel-Lindau Disease: First Case

We report a 47-year-old Japanese female with 10 previous treatments for multiple bilateral renal cell carcinoma (RCC) associated with von Hippel-Lindau disease. The 14-mm right lower pole renal tumor was in contact with the right ureter. Laparoscopic cryoablation was performed to protect the ureter wrapped with gauze. Computed tomography (CT) monitoring was used to confirm the precise ≥ 6 mm ice-ball margin. There was no local progression at 6-months post-surgery. The serum creatinine has been stable. This is apparently the first report of combined laparoscopic and CT monitoring of an ice-ball formation and its margin during cryoablation for RCC

Role of the boundary in feather bud formation on one-dimensional bioengineered skin

The role of a boundary in pattern formation from a homogenous state in Turing's reaction–diffusion equations is important, particularly when the domain size is comparable to the pattern scale. Such experimental conditions may be achieved for in vitro regeneration of ectodermal appendages such as feathers, via reconstruction of embryonic single cells. This procedure can eliminate a predefined genetic map, such as the midline of chick feather bud formation, leaving uniformly distributed identical cells as a bioengineered skin. Here, the self-organizing nature of multiple feather bud formation was examined in bioengineered 1D-skin samples. Primal formation of feather buds occurred at a fixed length from the skin edge. This formation was numerically recapitulated by a standard two-component reaction-diffusion model, suggesting that the boundary effect caused this observation. The proper boundary conditions were nonstandard, either mixed Dirichlet–Neumann or partial-flux. In addition, the model implies imperfect or hindered bud formation as well as nearly equal distances between buds. In contrast, experimental observations indicated that the skin curvature, which was not included in our model, also strongly affected bud formation. Thus, bioengineered skin may provide an ideal template for modeling a self-organized process from a homogenous state. This study will examine the possible diffusion activities of activator or inhibitor molecular candidates and mechanical activities during cell aggregation, which will advance our understanding of skin appendage regeneration from pluripotent or embryonic stem cells

Psychometric properties of the eating disorder examination-questionnaire in Japanese adolescents

Abstract Background Although the Eating Disorder Examination Questionnaire version 6.0 (EDE-Q) is one of the most widely used questionnaires for eating disorders in Western countries, no research has addressed the psychometric properties of the EDE-Q in a Japanese sample. Methods We explored the factor structure of the EDE-Q and examined the internal consistency of the derived scales for Japanese participants (Study I), the convergent validity with other eating disorder-related psychological measures (Study II) and the distinction between the derived two body image-related factors with psychological measures (StudyIII). The EDE-Q was administered to 1,430 undergraduate students in Study I and in Study II was subsequently assessed by two self-report measures of eating pathology, the Eating Attitudes Test (EAT-26) for 558 undergraduate students and the Eating Disorders Inventory-II (EDI-II) 111. In StudyIII, another 225 undergraduate students participated in an examination of the relationships of the derived body image-related subscales of the EDE-Q with the psychological measures of the Rosenberg Self-Esteem Scale, Beck Depression Inventory, Public Self-Consciousness Scale, and Multidimensional Perfectionism Scale. Results Exploratory factor analysis of the EDE-Q identified four meaningful factors. Of the original four EDE-Q factors, “Restriction” and “Eating Concern” were retained. However, the other two factors, “Shape” and “Weight” Concerns, were combined into two different factors: “Fear of Obesity” and “Self-Esteem Based on Shape and Weight”. Internal consistency of the derived four factors was adequate, and the relationships with EDI-II and EAT-26 measures demonstrated convergent validity. Analysis of the distinction between “Fear of Obesity” and “Self-Esteem Based on Shape and Weight” revealed that only “Self-Esteem Based on Shape and Weight” was significantly associated with the measures assessing psychopathology related to eating disorders. Conclusions This study describes restructured factors of the EDE-Q that were tested with undergraduate students. The distinction between two factors, “Fear of Obesity” and “Self-Esteem Based on Shape and Weight”, may further the understanding of the psychopathology of the eating disorders of adolescent Japanese subjects to facilitate future developments in research and treatment