酵素電極触媒型NADPH酸化反応を共役したペントースリン酸経路の再構成

Biofuel cell is a next-generation energy device that generates the electricity from renewable fuels such as glucose using redox enzymes as electrode catalyses. We re-constructed in vitro pentose phosphate pathway for 24-e oxidation of glucose-6-phosphate (G6P) into CO2 and linked it to mediated bioelectrocatalytic NADPH oxidation system in order to improve the energy density of biofuel cells. The electrolysis efficiency reached to 40% in the G6P oxidation by adjusting the composition of the enzymes in the pathway

積層型シート状酵素バイオ電池の構築

We have constructed a sheet-type glucose/dioxygen enzymatic biofuel cell with multi-stacked structure, in which one biocathode is sandwiched with two bioanodes in parallel. The energy density of the biofuel cell reached 14.1 mWh cm−3. The cell has achieved the acceptable performance on re-fueling, and the stability of the cell before use was drastically improved by adding raffinose