In vitro studies on effects of plant growth regulators on callus and suspension culture biomass yield from Gymnema sylvestre R.Br

Callus cultures were initiated from nodal segments and leaf explants of Gymnema sylvestre on Murashige and Skoog (1962) medium containing basic salts and 30 g/l sucrose supplemented with different concentrations (0.10, 0.25, 0.5, 1.0, 2.0 and 5.0 mg/l) of 2,4-dichlorophenoxy acetic acid (2,4-D), -naphthalene acetic acid (NAA), indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), kinetin (KN) and 6-benzyladenine (BA). Callus induction was observed in 0.5 mg/l of 2, 4-D supplemented medium for both explants. At the initial stage, some parts of explants enlarged and gave raise to pale yellowish calli after 2-3 weeks of incubation. The harvested cell biomass was subjected to extraction of active principles. In this study, cell biomass extracts were compared with extracts from leaves of naturally growing gymnema plants. HPLC analysis of these extracts showed that the main components of the active principles namely gymnemic acids and gymnemagenin were present in sufficiently large amounts in the cultured undifferentiated cell

Genome sequence of Citrobacter sp strain A1, A dye-degrading bacterium

Citrobacter sp. strain A1, isolated from a sewage oxidation pond, is a facultative aerobe and mesophilic dye-degrading bacterium. This organism degrades azo dyes efficiently via azo reduction and desulfonation, followed by the successive biotransformation of dye intermediates under an aerobic environment. Here we report the draft genome sequence of Citrobacter sp. A1