9 research outputs found
<span style="font-size:15.0pt;mso-bidi-font-size: 12.0pt;font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:Mangal;mso-ansi-language:EN-GB;mso-fareast-language:EN-US; mso-bidi-language:HI" lang="EN-GB">Aqueous extract of <i style="mso-bidi-font-style:normal">Allium sativum</i> L bulbs offer nephroprotection by attenuating vascular endothelial growth factor and extracellular signal-regulated kinase-1 expression in diabetic rats</span>
139-148<span style="font-size:
9.0pt;mso-bidi-font-size:12.0pt" lang="EN-GB">To investigate the nephroprotective effect of
garlic and elucidate the mechanism by which it prevents the
progression of diabetic nephropathy in diabetic rats, diabetes was induced by a
single ip injection of streptozotocin
(45 mg/kg body weight). Garlic extract (500 mg/kg body weight) and
aminoguanidine (1 g/L) were supplemented in the treatment groups.
Histopathological examination using H&E, PAS staining and the
immunohistochemical analysis of vascular endothelial growth factor (VEGF) and
extracellular signal-regulated kinase-1 (ERK-1) expression were performed on
kidney sections at the end of 12 weeks. Significant change in both, the urine
and serum biochemistry confirmed kidney damage in diabetic animals which was
further confirmed by the histological changes such as mesangial expansion,
glomerular basement membrane thickening, glycosuria and proteinuria. However,
the diabetic animals treated with garlic extract showed a significant change in
urine and serum biochemical parameters such as albumin, urea nitrogen and creatinine compared to that of diabetic rats. Further,
the garlic supplemented diabetic rats showed a significant decrease in the
expression of VEGF and ERK-1 compared to diabetic rats, attenuating mesangial
expansion and glomerulosclerosis. Thus, garlic extract rendered
nephroprotection in diabetic rats.
</span
CD36 in chronic kidney disease: novel insights and therapeutic opportunities
CD36 (also known as scavenger receptor B2) is a multifunctional receptor that mediates the binding and cellular uptake of long-chain fatty acids, oxidized lipids and phospholipids, advanced oxidation protein products, thrombospondin and advanced glycation end products, and has roles in lipid accumulation, inflammatory signalling, energy reprogramming, apoptosis and kidney fibrosis. Renal CD36 is mainly expressed in tubular epithelial cells, podocytes and mesangial cells, and is markedly upregulated in the setting of chronic kidney disease (CKD). As fatty acids are the preferred energy source for proximal tubule cells, a reduction in fatty acid oxidation in CKD affects kidney lipid metabolism by disrupting the balance between fatty acid synthesis, uptake and consumption. The outcome is intracellular lipid accumulation, which has an important role in the pathogenesis of kidney fibrosis. In experimental models, antagonist blockade or genetic knockout of CD36 prevents kidney injury, suggesting that CD36 could be a novel target for therapy. Here, we discuss the regulation and post-translational modification of CD36, its role in renal pathophysiology and its potential as a biomarker and as a therapeutic target for the prevention of kidney fibrosis