34 research outputs found
Septal contributions to olfactory bulb interneuron diversity in the embryonic mouse telencephalon: role of the homeobox gene Gsx2
Evidence that the Type-2 Gonadotrophin-Releasing Hormone (GnRH) Receptor Mediates the Behavioural Effects of GnRH-II on Feeding and Reproduction in Musk Shrews
PAX6 haploinsufficiency causes cerebral malformation and olfactory dysfunction in humans
Molecular Polymorphism of Native Gonadotropin-Releasing Hormone (GnRH) Is Restricted to Mammalian GnRH and [Hydroxyproline<sup>9</sup>] GnRH in the Developing Rat Brain
Colchicine-lnduced Accumulation of Estrogen Receptor and Progestin Receptor Immunoreactivity in Atypical Areas in Guinea-Pig Brain
Development of the blood–brain barrier within the paraventricular nucleus of the hypothalamus: influence of fetal glucocorticoid excess
miR-7a regulation of Pax6 controls spatial origin of forebrain dopaminergic neurons.
International audienceIn the postnatal and adult mouse forebrain, a mosaic of spatially separated neural stem cells along the lateral wall of the ventricles generates defined types of olfactory bulb neurons. To understand the mechanisms underlying the regionalization of the stem cell pool, we focused on the transcription factor Pax6, a determinant of the dopaminergic phenotype in this system. We found that, although Pax6 mRNA was transcribed widely along the ventricular walls, Pax6 protein was restricted to the dorsal aspect. This dorsal restriction was a result of inhibition of protein expression by miR-7a, a microRNA (miRNA) that was expressed in a gradient opposing Pax6. In vivo inhibition of miR-7a in Pax6-negative regions of the lateral wall induced Pax6 protein expression and increased dopaminergic neurons in the olfactory bulb. These findings establish miRNA-mediated fine-tuning of protein expression as a mechanism for controlling neuronal stem cell diversity and, consequently, neuronal phenotype