28 research outputs found
Multiscale multifactorial approaches for engineering tendon substitutes
The physiology of tendons and the continuous strains experienced daily make tendons very prone to injury. Excessive and prolonged loading forces and aging also contribute to the onset and progression of tendon injuries, and conventional treatments have limited efficacy in restoring tendon biomechanics. Tissue engineering and regenerative medicine (TERM) approaches hold the promise to provide therapeutic solutions for injured or damaged tendons despite the challenging cues of tendon niche and the lack of tendon-specific factors to guide cellular responses and tackle regeneration. The roots of engineering tendon substitutes lay in multifactorial approaches from adequate stem cells sources and environmental stimuli to the construction of multiscale 3D scaffolding systems.
To achieve such advanced tendon substitutes, incremental strategies have been pursued to more closely recreate the native tendon requirements providing structural as well as physical and chemical cues combined with biochemical and mechanical stimuli to instruct cell behavior in 3D architectures, pursuing mechanically competent constructs with adequate maturation before implantation.Authors acknowledge the project “Accelerating tissue engineering and personalized medicine discoveries by the integration of key enabling nanotechnologies, marinederived biomaterials and stem cells,” supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the Portugal 2020 Partnership Agreement, through the European
Regional Development Fund (ERDF).
Authors acknowledge the H2020 Achilles Twinning Project No. 810850, and also the European
Research Council CoG MagTendon No. 772817, and the FCT Project MagTT PTDC/CTM-CTM/
29930/2017 (POCI-01-0145-FEDER-29930
Absence of mitochondrial and chloroplast DNA recombinations in Brassica napus plants regenerated from protoplasts, protoplast fusions and anther culture
Towards the genetic engineering of triacylglycerols of defined fatty acid composition: Major changes in erucic acid content at the sn-2position affected by the introduction of a1-acyl-sn-glycerol-3-phosphate acyltransferase from Limnanthesdouglasii into oi
Mitochondrial DNA rearrangements in somatic hybrids of Solanum tuberosum and Solanum brevidens
Stable inheritance and expression of the CMS traits introduced by asymmetric protoplast fusion
Fertility of somatic hybrids from protoplast fusions of Solanum brevidens and S. tuberosum
Plant gene transfer
The production of transgenic plants requires the transfer of genetic material into a plant cell, stable integration of the introduced DNA in the genome of the plant and regeneration of whole plants such that the introduced gene is stably maintained in subsequent generations..