12 research outputs found

    Vacuole-Targeted Proteins: Ins and Outs of Subcellular Localization Studies

    No full text
    International audienceAccurate and efficient demonstrations of protein localizations to the vacuole or tonoplast remain strict prerequisites to decipher the role of vacuoles in the whole plant cell biology and notably in defence processes. In this chapter, we describe a reliable procedure of protein subcellular localization study through transient transformations of Catharanthus roseus or onion cells and expression of fusions with fluorescent proteins allowing minimizing artefacts of targeting

    Compartmentation of Alkaloid Synthesis, Transport, and Storage

    No full text
    corecore