41 research outputs found
Comparative expression profiling reveals a role of the root apoplast in local phosphate response
Effects of Calmodulin Antagonists on Transmembrane Auxin Transport in Cucurbita Pepo L. Hypocotyl Segments
Stimulation of In Vitro H+ Transport in Zucchini Microsomes by the Ether Lipid Platelet Activating Factor and a Soluble Protein
Orientation of NAHD-linked ferric chelate (turbo) reductase in plasma membranes from roots ofPlantago lanceolata
Membrane traffic from the endoplasmic reticulum to the Golgi apparatus is disturbed by an inhibitor of the Ca2+-ATPase in the ER
Genetic analysis of iron chlorosis tolerance in Prunus rootstocks
39 Pags., 4 Tabls., 4 Figs. The definitive version is available at: http://link.springer.com/journal/11295The high economic losses caused by the occurrence of iron chlorosis in Prunus orchards in the Mediterranean area justifies the implementation of breeding programs to generate high-performance rootstocks for different edaphoclimatic area conditions. For that reason, the genetic control of iron chlorosis tolerance was studied in an F1 population derived from a three-way interspecific cross between a Myrobalan plum (P 2175) and an almond × peach hybrid (Felinem). Several phenotypic measurements were assessed to guarantee an accurate data set for genetic analysis. SPAD (Soil and Plant Analyzer Development) values, chlorophyll concentration, and visual diagnostic symptoms were highly correlated with leaf chlorosis in trees. SPAD value was the most reliable measure, since it was an objective, unbiased, and non-destructive method. Two significant quantitative trait loci (QTLs) involved in SPAD and chlorophyll concentration were identified for Felinem in linkage groups 4 and 6. Both QTLs were detected in four of the six consecutive years of the experiment. For P 2175, two of the three putative QTLs identified, pspad4.1 and chl4.1, were placed in linkage group 4. These QTLs were related to the SPAD values and chlorophyll concentration, respectively, and co-localized with QTLs detected in the Felinem map affecting the same traits. Candidate gene PFIT, related to iron metabolism, was localized within the confidence interval of the QTL in linkage group 4. This research suggests an association of this chromosome region with tolerance to iron chlorosis in Prunus, and it provides a first approach to localize candidate genes involved in tolerance to this abiotic stress.This research was funded by MICINN (Spanish Ministry of Science and Innovation, AGL 2008-00283) and co-funded with a FEDER project and Gobierno de Aragón (A44). M.J. Gonzalo was the beneficiary of an I3P-PC2006 contract from the CSIC-FSE.Peer reviewe