Selection and genetic characterization of Beauveria bassiana by RAPD analysis for the control of Homalinotus coriaceus Sele?ao e caracteriza?ao gen?tica por RAPD de linhagens de Beauveria bassiana para o controle de Homalinotus coriaceus

3 ilus. 1 tab. 12 ref.La patogenicidad de veintidos linajes de Beauveria bassiana fue probada sobre adultos de Homalinotus coriaceus, cuyas larvas atacan el ped?nculo floral del coquero, ocasionando la ca?da de las flores y de los frutos inmaduros. El trabajo se desarroll? en Embrapa-CPATC y Embrapa-CENARGEM, siguiendo un dise?o experimental completamente aleatorizado, con 23 tratamientos (22 linajes y un testigo) y cinco repeticiones, cada una con 20 insectos adultos. La prueba de patogenicidad fue realizada por la inmersi?n de adultos de H. coriaceus en suspensi?n conidial (10 exponente 9 conidios/mL). Los aislados fueron caracterizados gen?ticamente por RAPD, utilizando 16 imprimadores. Los linajes seleccionados, con patogenicidad superior a 80 porciento, fueron: CG002, CG544, CG817, CG557 y CG219. La caracterizaci?n gen?tica indic? correlaci?n entre el dendrograma y la matriz, con una similitud de 95 porciento. El linaje m?s patog?nico (CG002) present? 67 porciento de similitud con el cuarto linaje m?s patog?nico (CG817), mientras que los dem?s linajes seleccionados presentaron una similitud menor al 67 porciento. Los an?lisis de RAPD no produjeron grupos bien definidos, concluy?ndose que la virulencia de B. bassiana a H. coriaceus no tiene relaci?n con la similitud gen?tica verificada entre los linajes. The patogenicity of twenty-two Beauveria bassiana strains was evaluated against adults of Homalinotus coriaceus (Coleoptera: Curculionidae), an important coconut pest in Brazil whose larvae attack the bunch, causing the flowers and the immature fruits to fall down. The research was carried out in Embrapa-CPATC and Embrapa-CENARGEM, following a completely randomized design, with 23 treatments (22 strains and a blank) and five replications, with 20 adult insects each. The pathogenicity test was carried out by immersing H. coriaceus adults in a conidial suspension (10 exponent 9 conidia/mL). The strains were genetically identified through RAPD, using 16 primers. The strains with a patogenicity of over 80 percent were: CG002, CG544, CG817, CG557, and CG219.The genetic characterization showed a correlation of 95 percent between the dendogram and the similarity matrix. The most pathogenic strain (CG002) showed a similarity of 67 percent with the fourth one (CG817). The other strains showed a similarity of under 67 percent. The RAPD analysis did not show well-defined groups, and we conclude that B. bassiana virulence to H. coriaceus is not correlated with genetic similarity between strains

Analise de patogenicidade e germinacao do fungo Nomurae rileyi (Farlow) Samson isolados no Distrito Federal.

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Molecular characterization of Paecilomyces fumosoroseus (Deuteromycotina: Hyphomycetes) isolates Caracterização molecular de isolados de Paecilomyces fumosoroseus (Deuteromycotina: Hyphomycetes)

ITS and RAPD analyses were used to investigate molecular variations within samples of Paecilomyces isolates and to resolve five morphologically atypical isolates resembling P. fumosorosus, obtained from whitefly in Northern Paraná State. The ITS4-ITS5 amplicon was 700 base pairs (bp) long in all isolates. The five isolates of Paecilomyces not assigned to species produced restriction profiles identical to all the reference strains of P. fumosoroseus. The extent of fingerprint variability observed by RAPD was sufficient to discriminate all the isolates. The genetic similarity among unidentified isolates and strains of P. fumosoroseus was even higher than that observed among reference strains of this species, allowing us to conclude that isolates CNPso-P77, CNPso-P78, CNPso-P80, CNPso-P85 and CNPso-P91 are P. fumosoroseus.<br>As análises de RAPD (Polimorfismo de DNA Amplificado ao Acaso) e ITS (Sequências Internas Transcritas) foram utilizadas para investigar a variabilidade molecular entre isolados de Paecilomyces e para identificar cinco isolados morfologicamente atípicos, obtidos a partir de mosca branca no Norte do Estado do Paraná, que possuíam alguma semelhança com P. fumosoroseus. O produto da amplificação com os primers ITS4 e ITS5 apresentou 700 pares de bases para todos os isolados investigados. Os cinco isolados não identificados deram origem a padrões de restrição idênticos a todas as linhagens de P. fumosoroseus utilizadas como referência. A variabilidade observada nos perfis de RAPD foi suficiente para discriminar todos os isolados. A similaridade genética entre os isolados não identificados e as linhagens de P. fumosoroseus foi maior do que aquela observada entre as linhagens referência desta espécie. Este fato permitiu concluir que os isolados CNPso-P77, CNPso-P78, CNPso-P80, CNPso-P85 and CNPso-P91 pertencem à espécie P. fumosoroseus