Determination of benzo[alpha]pyrene in Turkish döner kebab samples cooked with charcoal or gas fire

peer-reviewedIn order to investigate the levels of the potent carcinogen benzo[a]pyrene (B(a)P), 40 samples of döner kebab were analysed. The samples tested included 20 cooked using a charcoal fire and 20 cooked using a gas fire. A liquid chromatographic method was developed using a fluorescence detector. The mean levels of B(a)P were found to be 24.2 (s.e. 0.84) g/kg for charcoal fire cooked meat samples and 5.7 (s.e. 3.48 g/kg) for gas fire cooked meat samples. Sixteen samples were found to be over the maximum level recommended by FAO/WHO (10 g/kg) and all of the samples exceeded the maximum tolerance level of the Turkish Food Codex (1 g/kg)

An integrated approach project for the revaluation of a traditional sourdough bread production chain

The influence of organic and conventional farming systems on the performance of a panel of old and modern Italian bread wheat varieties has been evaluated, with the aim to individuate an agronomic protocol suitable for the production of a sourdough bread traditionally prepared in a hill zone of Emilia-Romagna. The agronomic and technological characterisation of the wheat samples obtained in organic and conventional farming conditions has been done and the sensorial qualities of the sourdough bread obtained have been evaluated

The Notch pathway is a critical regulator of angiogenesis in a skin model of ischemia

WOS: 000355334000001PubMed ID: 25834117The Notch pathway is definitely required for normal vascular development. Although the contribution of Notch in postnatal angiogenesis is the focus of intense investigation, the implication of Notch in reparative neovascularization in the skin remains unexplored. In this study, we investigated Notch changes using a skin model of ischemia. Thirty Sprague-Dawley rats were divided into two groups. In the surgery group (n = 24), a caudally based dorsal skin flap was raised and sutured back into its initial position. In the control group, no surgical procedure was performed. Tissue biopsies were obtained at different time intervals. Tissue specimens were assessed for Delta-like ligand 4 (DLL4) and vascular endothelial growth factor (VEGF) gene expression by real-time polymerase chain reaction (PCR). Immunohistochemical staining was used for detection of DLL4 in tissue materials. Quantitative assessment of skin flap microvasculature was made. Compared with normoperfused tissue, VEGF and DLL4 expressions increased significantly (p < 0.01). Immunohistochemical analysis revealed weak and patchy expression of DLL4 in microvascular endothelial cells of normoperfused tissues. Conversely, DLL4 expression was upregulated in capillary endothelial cells after ischemia. In conclusion, in this study we have shown that the Notch ligand DLL4 is upregulated in skin tissue after ischemia. A deeper understanding of these fundamental principles will aid in the development of new avenues for the treatment of blood vessel-related skin pathologies.Baskent University Research FundBaskent UniversityThis study was approved by Baskent University Ethical Committee for Experimental Research on Animals and supported by Baskent University Research Fund

Failure of Sterne- and Pasteur-Like Strains of Bacillus anthracis to Replicate and Survive in the Urban Bluebottle Blow Fly Calliphora vicina under Laboratory Conditions

Britta von Terzi, Peter C. B. Turnbull, Wolfgang Beyer, University of Hohenheim, Institute of Environmental and Animal Hygiene, Stuttgart, GermanySteve E. Bellan, Center for Computational Biology and Bioinformatics, University of Texas at Austin, Austin, Texas, United States of AmericaThis study aimed to elucidate the bacteriological events occurring within the gut of Calliphora vicina, selected as the European representative of blow flies held responsible for the spread of anthrax during epidemics in certain parts of the world. Green-fluorescent-protein-carrying derivatives of Bacillus anthracis were used. These lacked either one of the virulence plasmids pXO1 and pXO2 and were infected, or not infected, with a worm intestine phage (Wip4) known to influence the phenotype and survival of the pathogen. Blood meals were prepared for the flies by inoculation of sheep blood with germinated and, in case of pXO2+ strains, encapsulated cells of the four B. anthracis strains. After being fed for 4 h an initial 10 flies were externally disinfected with peracetic acid to ensure subsequent quantitation representing ingested B. anthracis only. Following neutralization, they were crushed in sterile saline. Over each of the ensuing 7 to 10 days, 10 flies were removed and processed the same way. In the absence of Wip4, strains showed steady declines to undetectable in the total B. anthracis counts, within 7–9 days. With the phage infected strains, the falls in viable counts were significantly more rapid than in their uninfected counterparts. Spores were detectable in flies for longer periods than vegetative bacteria. In line with the findings in both biting and non-biting flies of early workers our results indicate that B. anthracis does not multiply in the guts of blow flies and survival is limited to a matter of days.This work was funded by grant BE 2157/3-1 of the German Research Foundation (DFG). SEB was funded by a United States National Institute of General Medical Sciences MIDAS grant U01GM087719 to Lauren A. Meyers and Alison P. Galvani. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Center for Computational Biology and BioinformaticsEmail: [email protected]

Using the D-BEST Reference Model to Compare Italian and Polish Digital Innovation Hubs

In recent years, the increasing importance of Digital Innovation Hubs (DIHs) in supporting manufacturing Small and Medium Enterprises (SMEs) has been widely studied and several works listing lessons learnt and success stories have been published. To further foster the impact of these entities on the SMEs’ environment, The European Commission has recently introduced the Smart Specialisation Platform, which contains a web service returning to its users a geo-distributed list of DIHs, allowing the user also to cluster and visualise them according to some pre-defined filters, such as the types of technologies employed. The data provided by this platform has been downloaded and a secondary data analysis, based on the websites of the DIHs has been carried on to frame the single DIHs according to the axes of the D-BEST methodology. A comparative analysis with respect to the Italian and Poland situation completes the study, to understand eventual differences and affinities among the two countries

Food industry digitalization: from challenges and trends to opportunities and solutions

Over the last years, manufacturing companies have to face several challenges, mainly related to the volatility of the demand and to the continuously changing requirements, both from the customers and suppliers. In the meantime, new technological roadmaps and suggested interventions in manufacturing systems have been implemented. These solutions aim to exploit the high innovation and economic potential resulting from the continuing impact of rapidly advancing information and communication technology (ICT) in industry. This paper explores these topics focusing on the food sector. Indeed, companies belonging to this industry are facing global challenges, which can be met with the support of the information technologies (IT). The overall goal of this study is to help food companies toward digitalization, with a particular focus on the design and manufacturing processes. From the methodological point of view, Case Study has been used as research method. Furthermore, a questionnaire characterized by the different elements of the Manufacturing Value Modelling Methodology (MVMM) has been developed and used to gather information from companies. A framework for the digitalization process in the food industry has been developed basing on the results of a preliminary literature review and of different focus groups. On completion of the aforementioned framework, a list of enabling technologies has been discussed. These represent the technological solutions for the specific food issues highlighted by the framework. Finally, a case study has been accomplished in order to test and validate the contents\u2019 framework

Food industry digitalization: from challenges and trends to opportunities and solutions

Over the last years, manufacturing companies have to face several challenges, mainly related to the volatility of the demand and to the continuously changing requirements, both from the customers and suppliers. In the meantime, new technological roadmaps and suggested interventions in manufacturing systems have been implemented. These solutions aim to exploit the high innovation and economic potential resulting from the continuing impact of rapidly advancing information and communication technology (ICT) in industry. This paper explores these topics focusing on the food sector. Indeed, companies belonging to this industry are facing global challenges, which can be met with the support of the information technologies (IT). The overall goal of this study is to help food companies toward digitalization, with a particular focus on the design and manufacturing processes. From the methodological point of view, Case Study has been used as research method. Furthermore, a questionnaire characterized by the different elements of the Manufacturing Value Modelling Methodology (MVMM) has been developed and used to gather information from companies. A framework for the digitalization process in the food industry has been developed basing on the results of a preliminary literature review and of different focus groups. On completion of the aforementioned framework, a list of enabling technologies has been discussed. These represent the technological solutions for the specific food issues highlighted by the framework. Finally, a case study has been accomplished in order to test and validate the contents' framework. (C) 2018, IFAC (International Federation of Automatic Control) Hosting by Elsevier Ltd. All rights reserved

Digital Innovation Hubs Proposing Digital Platforms to Lead the SMEs Digital Transition

DIHs could offer a marketplace and play the role of a broker facilitating cooperation and networking among important stakeholders, harmonizing companies with customers and providing European connections as well. In this framework, DIHs express an increasing need for having platforms bolstering users-producers transactions, fostering to detect what is needed, by grouping a large variety of services and goods together. This paper presents the main platforms grounded on the D-BEST reference model developed by different networks of DIHs. The main characteristics of each of them have been detected and the differences among them unveiled

Industry 4.0 Driven Result-oriented PSS: An Assessment in the Energy Management

Sustainability is a current challenge and all sectors, including the buildings one, are being called upon to provide a solution to mitigate climate change. The state of the art of energy management using Information and Communication Technologies (ICT) technology in building systems industry is characterized by a traditional monitoring approach which could assess the energy consumption of the building but that cannot manage and act the required action to improve the energy management according to a demand side approach. The aim of this paper is to overcome this traditional monitoring approach, presenting Simon, a new model proposed by Evogy, enabling a result-oriented product service system (PSS) for buildings through cyber physical systems, artificial intelligence, and internet of things adoption. The main findings highlight the benefits associated with the Simon model by virtue of digital-based predictive maintenance on the real system. In addition, its adoption allows the PSS provider to aggregate energy demand from the plethora of buildings and, according to digital technologies, not only monitor consumption but also implement equipment. Finally, the application case highlights the benefits are different and thus stands as a best practice for combining sustainability and digitization