48 research outputs found
Biodegradable polydioxanone stents in the treatment of adult patients with tracheal narrowing
Implantation of Mouse Embryonic Stem Cell-Derived Cardiac Progenitor Cells Preserves Function of Infarcted Murine Hearts
Stem cell transplantation holds great promise for the treatment of myocardial infarction injury. We recently described the embryonic stem cell-derived cardiac progenitor cells (CPCs) capable of differentiating into cardiomyocytes, vascular endothelium, and smooth muscle. In this study, we hypothesized that transplanted CPCs will preserve function of the infarcted heart by participating in both muscle replacement and neovascularization. Differentiated CPCs formed functional electromechanical junctions with cardiomyocytes in vitro and conducted action potentials over cm-scale distances. When transplanted into infarcted mouse hearts, CPCs engrafted long-term in the infarct zone and surrounding myocardium without causing teratomas or arrhythmias. The grafted cells differentiated into cross-striated cardiomyocytes forming gap junctions with the host cells, while also contributing to neovascularization. Serial echocardiography and pressure-volume catheterization demonstrated attenuated ventricular dilatation and preserved left ventricular fractional shortening, systolic and diastolic function. Our results demonstrate that CPCs can engraft, differentiate, and preserve the functional output of the infarcted heart
BCRP expression does not result in resistance to STX140 in vivo, despite the increased expression of BCRP in A2780 cells in vitro after long-term STX140 exposure
The anti-proliferative and anti-angiogenic properties of the endogenous oestrogen metabolite, 2-methoxyoestradiol (2-MeOE2), are enhanced in a series of sulphamoylated derivatives of 2-MeOE2. To investigate possible mechanisms of resistance to these compounds, a cell line, A2780.140, eightfold less sensitive to the 3,17-O,O-bis-sulphamoylated derivative, STX140, was derived from the A2780 ovarian cancer cell line by dose escalation. Other cell lines tested did not develop STX140 resistance. RT–PCR and immunoblot analysis demonstrated that breast cancer resistance protein (BCRP) expression is dramatically increased in A2780.140 cells. The cells are cross-resistant to the most structurally similar bis-sulphamates, and to BCRP substrates, mitoxantrone and doxorubicin; but they remain sensitive to taxol, an MDR1 substrate, and to all other sulphamates tested. Sensitivity can be restored using a BCRP inhibitor, and this pattern of resistance is also seen in a BCRP-expressing MCF-7-derived cell line, MCF-7.MR. In mice bearing wild-type (wt) and BCRP-expressing tumours on either flank, both STX140 and mitoxantrone inhibited the growth of the MCF-7wt xenografts, but only STX140 inhibited growth of the MCF-7.MR tumours. In conclusion, STX140, a promising orally bioavailable anti-cancer agent in pre-clinical development, is highly efficacious in BCRP-expressing xenografts. This is despite an increase in BCRP expression in A2780 cells in vitro after chronic dosing with STX140
The Liver as Modulator of the Host-Defense Response: Host-Defense Failure Disease as a Manifestation of Hepatic Decompensation
Estrogen Potentiates the Combined Effects of Transforming Growth Factor-β and Tumor Necrosis Factor-α on Adult Human Osteoblast-like Cell Prostaglandin E2 Biosynthesis
An oxindole efflux inhibitor potentiates azoles and impairs virulence in the fungal pathogen Candida auris
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Feo - Transport of ferrous iron into bacteria
Bacteria commonly utilise a unique type of transporter, called Feo, to specifically acquire the ferrous (Fe2+) form of iron from their environment. Enterobacterial Feo systems are composed of three proteins: FeoA, a small, soluble SH3-domain protein probably located in the cytosol; FeoB, a large protein with a cytosolic N-terminal G-protein domain and a C-terminal integral inner-membrane domain containing two 'Gate' motifs which likely functions as the Fe2+ permease; and FeoC, a small protein apparently functioning as an [Fe-S]-dependent transcriptional repressor. We provide a review of the current literature combined with a bioinformatic assessment of bacterial Feo systems showing how they exhibit common features, as well as differences in organisation and composition which probably reflect variations in mechanisms employed and function
A novel nanoparticle formulation overcomes multiple types of membrane efflux pumps in human breast cancer cells
Efeito da atividade fÃsica no osso normal e na prevenção e tratamento da osteoporose Efectos de la actividad fÃsica en huesos normales y en la prevención y tratamiento de osteoporosis Effect of the physical activity on normal bone and on the osteoporosis prevention and treatment
A osteoporose é uma doença cada vez mais diagnosticada em mulheres e homens de todo o mundo. Embora os esteróides sexuais sejam importantes na gênese da osteoporose, a inatividade fÃsica constitui um fator de risco. O exercÃcio fÃsico atua no osso por efeito direto, via força mecânica, ou indireto, mediado por fatores hormonais. Mas os mecanismos pelos quais a atividade fÃsica melhora a massa óssea ainda não são totalmente conhecidos. Baseando-se nos resultados que demonstram os efeitos benéficos da atividade fÃsica no tecido ósseo, a prática de esportes vem sendo cada vez mais indicada na prevenção e até mesmo no tratamento da osteoporose. O objetivo desta revisão é descrever os efeitos da atividade fÃsica no tecido ósseo normal e na prevenção e tratamento da osteoporose.<br>La osteoporosis es una enfermedad que cada vez más se diagnostica en mujeres y hombres de todo el mundo. Aunque los esteroides sexuales sean importantes en la génesis de la osteoporosis, la inactividad fÃsica constituye un factor de riesgo. El ejercicio fÃsico actúa en el hueso de forma directa, vÃa fuerza mecánica, o indirecta, mediado por factores hormonales. Sin embargo la patogénesis por la que la actividad fÃsica mejora la masa ósea todavÃa no es totalmente conocida. Con base en los resultados que demuestran los efectos benéficos de la actividad fÃsica en el tejido óseo, la práctica de deportes viene siendo indicada cada vez más como medio de prevención y hasta incluso como tratamiento de la osteoporosis. El objetivo de esta revisión es describir los efectos de la actividad fÃsica en el tejido óseo normal y en la prevención y tratamiento de la osteoporosis.<br>Osteoporosis has been increasingly diagnosed in women and men worldwide. Although the sexual steroids are important in the genesis of human osteoporosis, it is believed that the lack of physical activity constitutes a risk factor. Physical activity acts on the bone by direct effect via mechanical force, or indirect effect through hormonal factors. However, the mechanism through which physical activity improves the bone mass is not completely known. Sports practice has been increasingly recommended for prevention and even treatment of osteoporosis based on the results that have demonstrated the beneficial effects of physical activity on the bone tissue. The goal of this review is to describe the effects of physical activity in the normal bone tissue and on the osteoporosis prevention and treatment