272 research outputs found
Formation of Galactic Center Magnetic Loops
A survey for the molecular clouds in the Galaxy with NANTEN mm telescope has
discovered molecular loops in the Galactic center region. The loops show
monotonic gradients of the line of sight velocity along the loops and the large
velocity dispersions towards their foot points. It is suggested that these
loops are explained in terms of the buoyant rise of magnetic loops due to the
Parker instability. We have carried out global three-dimensional
magneto-hydrodynamic simulations of the gas disk in the Galactic center. The
gravitational potential is approximated by the axisymmetric potential proposed
by Miyamoto & Nagai (1975). At the initial state, we assume a warm (~ 10^4 K)
gas torus threaded by azimuthal magnetic fields. Self-gravity and radiative
cooling of the gas are ignored. We found that buoyantly rising magnetic loops
are formed above the differentially rotating, magnetically turbulent disk. By
analyzing the results of global MHD simulations, we have identified individual
loops, about 180 in the upper half of the disk, and studied their statistical
properties such as their length, width, height, and velocity distributions
along the loops. Typical length and height of a loop are 1kpc and 200pc,
respectively. The line of sight velocity changes linearly along a loop and
shows large dispersions around the foot-points. Numerical results indicate that
loops emerge preferentially from the region where magnetic pressure is large.
We argue that these properties are consistent with those of the molecular loops
discovered by NANTEN.Comment: 16pages, 10figures. Accepted for publication in PASJ. Replace to
higher resolution versio
40Ar-39Ar analysis of phlogopite in the Horoman Peridotite Complex, Hokkaido, Japan and implications for its origin
金沢大学理工研究域自然システム学系40Ar-39Ar analysis of phlogopite separated from a plagioclase lherzolite of the Horoman Peridotite Complex, Hokkaido, Japan, has yielded a plateau age of 20.6 ± 0.5 Ma in an environment where the metamorphic fluid was characterized by an almost atmospheric Ar isotopic ratio. The age spectrum is slightly saddle-shaped, implying some incorporation of excess 40Ar during the formation of the phlogopite at a depth. As the phlogopite has been inferred to have formed in veins and/or interstitials during exhumation of the peridotite body, metasomatic fluids, to which ground- and sea water might have contributed, were probably involved in the formation of phlogopite in the crustal environment. A total 40Ar-39Ar age of 129 Ma of a whole rock sample of the plagioclase lherzolite, from which the phlogopite was separated and is representative of the main lithology of the Horoman Peridotite Complex, indicates the occurrence of excess 40Ar. Hence, the age has no geological meaning
Three novel oligosaccharides synthesized using Thermoanaerobacter brockii kojibiose phosphorylase
<p>Abstract</p> <p>Background</p> <p>Recently synthesized novel oligosaccharides have been produced primarily by hydrolases and glycosyltransferases, while phosphorylases have also been subject of few studies. Indeed, phosphorylases are expected to give good results via their reversible reaction. The purpose of this study was to synthesis other novel oligosaccharides using kojibiose phosphorylase.</p> <p>Results</p> <p>Three novel oligosaccharides were synthesized by glucosyltransfer from β-D-glucose 1-phosphate (β-D-G1P) to xylosylfructoside [<it>O</it>-α-D-xylopyranosyl-(1→2)-β-D-fructofuranoside] using <it>Thermoanaerobacter brockii </it>kojibiose phosphorylase. These oligosaccharides were isolated using carbon-Celite column chromatography and preparative high performance liquid chromatography. Gas liquid chromatography analysis of methyl derivatives, MALDI-TOF MS and NMR measurements were used for structural characterisation. The <sup>1</sup>H and <sup>13</sup>C NMR signals of each saccharide were assigned using 2D-NMR including COSY (correlated spectroscopy), HSQC (herteronuclear single quantum coherence), CH<sub>2</sub>-selected E-HSQC (CH<sub>2</sub>-selected Editing-HSQC), HSQC-TOCSY (HSQC-total correlation spectroscopy) and HMBC (heteronuclear multiple bond correlation).</p> <p>Conclusion</p> <p>The structure of three synthesized saccharides were determined, and these oligosaccharides have been identified as <it>O</it>-α-D-glucopyranosyl-(1→2)-<it>O</it>-α-D-xylopyranosyl-(1→2)-β-D-fructofuranoside (saccharide <b>1</b>), <it>O</it>-α-D-glucopyranosyl-(1→2)-<it>O</it>-α-D-glucopyranosyl-(1→2)-<it>O</it>-α-D-xylopyranosyl-(1→2)-β-D-fructofuranoside (saccharide <b>2</b>) and <it>O</it>-α-D-glucopyranosyl-(1→[2-<it>O</it>-α-D-glucopyranosyl-1]<sub>2</sub>→2)-<it>O</it>-α-D-xylopyranosyl-(1→2)-β-D-fructofuranoside (saccharide <b>3</b>).</p
Effect of three metal priming agents on the bond strength of adhesive resin cement to Ag-Zn-Sn-In alloy and component metals.
The aim of this study was to investigate the effect of three metal priming agents on the bond strength of adhesive resin cement to Silver-Zinc-Tin-Indium (Ag-Zn-Sn-In) alloy and pure Ag, Zn, Sn, and In. The specimens were air-abraded with alumina and then primed with one of three metal priming agents: V-Primer, Estenia Opaque Primer, or Alloy Primer. The metal disks were bonded with adhesive resin cement (Super-Bond Bulk-mix technique). Shear bond strengths (n=10/group) were determined before and after 50,000 thermocycles for Ag-Zn-Sn-In alloy as well as after 5,000 thermocycles for pure Ag, Zn, Sn and In. For Ag-Zn-Sn-In alloy, the post-thermocycling bond strength of the Alloy Primer group was significantly higher than that of the other primers. It can be concluded that Alloy Primer containing both the vinyl-thione monomer (VBATDT) and hydrophobic phosphate monomer (MDP) is effective for bonding Ag-Zn-Sn-In alloy and pure Ag, Zn and Sn.福岡歯科大学2017年
Ycf12 is a core subunit in the photosystem II complex
AbstractThe latest crystallographic model of the cyanobacterial photosystem II (PS II) core complex added one transmembrane low molecular weight (LMW) component to the previous model, suggesting the presence of an unknown transmembrane LMW component in PS II. We have investigated the polypeptide composition in highly purified intact PS II core complexes from Thermosynechococcus elongatus, the species which yielded the PS II crystallographic models described above, to identify the unknown component. Using an electrophoresis system specialized for separation of LMW hydrophobic proteins, a novel protein of ∼5 kDa was identified as a PS II component. Its N-terminal amino acid sequence was identical to that of Ycf12. The corresponding gene is known as one of the ycf (hypothetical chloroplast reading frame) genes, ycf12, and is widely conserved in chloroplast and cyanobacterial genomes. Nonetheless, the localization and function of the gene product have never been assigned. Our finding shows, for the first time, that ycf12 is actually expressed as a component of the PS II complex in the cell, revealing that a previously unidentified transmembrane protein exists in the PS II core complex
Discovery of Molecular Loop 3 in the Galactic Center: Evidence for a Positive-Velocity Magnetically Floated Loop towards
We have discovered a molecular dome-like feature towards and . The large velocity
dispersions of 50--100 km s of this feature are much larger than those
in the Galactic disk and indicate that the feature is located in the Galactic
center, probably within kpc of Sgr A. The distribution has a
projected length of pc and height of pc from the Galactic
disk and shows a large-scale monotonic velocity gradient of km s
per pc. The feature is also associated with HI gas having a
more continuous spatial and velocity distribution than that of CO. We
interpret the feature as a magnetically floated loop similar to loops 1 and 2
and name it "loop 3". Loop 3 is similar to loops 1 and 2 in its height and
length but is different from loops 1 and 2 in that the inner part of loop 3 is
filled with molecular emission. We have identified two foot points at the both
ends of loop 3. HI, CO and CO datasets were used to estimate the
total mass and kinetic energy of loop 3 to be \sim3.0 \times 10^{6} \Mo and
ergs. The huge size, velocity dispersions and energy
are consistent with the magnetic origin the Parker instability as in case of
loops 1 and 2 but is difficult to be explained by multiple stellar explosions.
We argue that loop 3 is in an earlier evolutionary phase than loops 1 and 2
based on the inner-filled morphology and the relative weakness of the foot
points. This discovery indicates that the western part of the nuclear gas disk
of kpc radius is dominated by the three well-developed magnetically
floated loops and suggests that the dynamics of the nuclear gas disk is
strongly affected by the magnetic instabilities.Comment: 30 pages, 10 figures. High resolution figures are available at
http://www.a.phys.nagoya-u.ac.jp/~motosuji/fujishita09_figs
A Detailed Observational Study of Molecular Loops 1 and 2 in the Galactic Center
Fukui et al. (2006) discovered two huge molecular loops in the Galactic
center located in (l, b) ~ (355 deg-359 deg, 0 deg-2 deg) in a large velocity
range of -180-40 km s^-1. Following the discovery, we present detailed
observational properties of the two loops based on NANTEN 12CO(J=1-0) and
13CO(J=1-0) datasets at 10 pc resolution including a complete set of velocity
channel distributions and comparisons with HI and dust emissions as well as
with the other broad molecular features. We find new features on smaller scales
in the loops including helical distributions in the loop tops and vertical
spurs. The loops have counterparts of the HI gas indicating that the loops
include atomic gas. The IRAS far infrared emission is also associated with the
loops and was used to derive an X-factor of 0.7(+/-0.1){\times}10^20 cm^-2 (K
km s^-1)^-1 to convert the 12CO intensity into the total molecular hydrogen
column density. From the 12CO, 13CO, H I and dust datasets we estimated the
total mass of loops 1 and 2 to be ~1.4 {\times} 106 Msun and ~1.9 {\times} 10^6
Msun, respectively, where the H I mass corresponds to ~10-20% of the total mass
and the total kinetic energy of the two loops to be ~10^52 ergs. An analysis of
the kinematics of the loops yields that the loops are rotating at ~47 km s-1
and expanding at ~141 km s^-1 at a radius of 670 pc from the center. Fukui et
al. (2006) presented a model that the loops are created by the magnetic
flotation due to the Parker instability with an estimated magnetic field
strength of ~150 {\mu}G. We present comparisons with the recent numerical
simulations of the magnetized nuclear disk by Machida et al. (2009) and
Takahashi et al. (2009) and show that the theoretical results are in good
agreements with the observations. The helical distributions also suggest that
some magnetic instability plays a role similarly to the solar helical features.Comment: 40 pages, 22 figures, submitted to publication in PAS
Similarity between the Molecular Loops in the Galactic Center and the Solar Chromospheric Arch Filaments
We carried out two-dimensional magnetohydrodynamic simulations of the
Galactic gas disk to show that the dense loop-like structures discovered by the
Galactic center molecular cloud survey by NANTEN 4 m telescope can be formed by
the buoyant rise of magnetic loops due to the Parker instability. At the
initial state, we assumed a gravitationally stratified disk consisting of the
cool layer ( K), warm layer ( K), and hot layer ( K). Simulation box is a local part of the disk containing the
equatorial plane. The gravitational field is approximated by that of a point
mass at the galactic center. The self-gravity, and the effects of the galactic
rotation are ignored. Numerical results indicate that the length of the
magnetic loops emerging from the disk is determined by the scale height of the
hot layer ( 100 pc at 1 kpc from the Galactic center). The loop length,
velocity gradient along the loops and large velocity dispersions at their foot
points are consistent with the NANTEN observations. We also show that the loops
become top-heavy when the curvature of the loop is sufficiently small, so that
the rising loop accumulates the overlying gas faster than sliding it down along
the loop. This mechanism is similar to that of the formation of solar
chromospheric arch filaments. The molecular loops emerge from the low
temperature layer just like the dark filaments observed in the H image
of the emerging flux region of the sun.Comment: 23 pages, 13 figures. Accepted for publication in PAS
Crucial role of vinexin for keratinocyte migration in vitro and epidermal wound healing in vivo.
In the process of tissue injury and repair, epithelial cells rapidly migrate and form epithelial sheets. Vinexin is a cytoplasmic molecule of the integrin-containing cell adhesion complex localized at focal contacts in vitro. Here, we investigated the roles of vinexin in keratinocyte migration in vitro and wound healing in vivo. Vinexin knockdown using siRNA delayed migration of both HaCaT human keratinocytes and A431 epidermoid carcinoma cells in scratch assay but did not affect cell proliferation. Induction of cell migration by scratching the confluent monolayer culture of these cells activated both EGFR and ERK, and their inhibitors AG1478 and U0126 substantially suppressed scratch-induced keratinocyte migration. Vinexin knockdown in these cells inhibited the scratch-induced activation of EGFR, but not that of ERK, suggesting that vinexin promotes cell migration via activation of EGFR. We further generated vinexin (-/-) mice and isolated their keratinocytes. They similarly showed slow migration in scratch assay. Furthermore, vinexin (-/-) mice exhibited a delay in cutaneous wound healing in both the back skin and tail without affecting the proliferation of keratinocytes. Together, these results strongly suggest a crucial role of vinexin in keratinocyte migration in vitro and cutaneous wound healing in vivo
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