High-spin [gamma]-ray spectroscopy in 198Bi, superdeformation in 191Au, and multi-photon resonances in low energy e+e- scattering

The properties of low-and high-spin levels in [superscript]198Bi have been studied with the [superscript]181Ta([superscript]22Ne,5n)[superscript]198Bi reaction at 116 MeV and 120 MeV bombarding energies. Levels along and near the yrast lines, including one isomer, were established up to spin J ≈ 30\hbar. The main configurations of these states are suggested to be [pi] h[subscript]9/2 coupled to the known excited states in [superscript]197Pb. A sequence of states built on the excitation of the odd proton (coupled to one, two, or three quasi neutron-holes) was observed. In addition, at least two collective bands were observed. These two bands are backbending bands and are suggested to be oblate collective structures built on oblate proton and possibly neutron states;A superdeformed (SD) band was observed for the first time in an Au isotope. The reaction used was [superscript]11B + [superscript]186W, demonstrating that very light ions can be used to populate SD bands at high angular momentum. The band is assigned to [superscript]191Au. The [gamma]-ray energies are at the so-called quarter-point energies of the [superscript]192Hg SD band, indicating that the [superscript]191Au SD band is identical to that of [superscript]192Hg;A search for resonant states in low-energy e[superscript]+e[superscript]- scattering through their decay to multi-photon final states was performed using e[superscript]+ from [superscript]68Ga sources and a Pb absorber. Energy-sum and invariant mass spectra of coincident 2[gamma] and 3[gamma] events were obtained using the 20-element High Energy-Resolution Array (HERA) facility. No evidence for resonant states was found, and upper limits for the partial decay widths of such resonances were established in the energy range from 1.1 to 1.8 MeV

Reversing binding sensitivity to A147T translocator protein

The translocator protein (TSPO) is a target for the development of neuroinflammation imaging agents. Clinical translation of TSPO PET ligands, such as [11C]DPA-713, has been hampered by the presence of a common polymorphism (A147T TSPO), at which all second-generation TSPO ligands lose affinity. Little is known about what drives binding at A147T compared to WT TSPO. This study aimed to identify moieties in DPA-713, and related derivatives, that influence binding at A147T compared to WT TSPO. We found changes to the nitrogen position and number in the heterocyclic core influences affinity to WT and A147T to a similar degree. Hydrogen bonding groups in molecules with an indole core improve binding at A147T compared to WT, a strategy that generated compounds that possess up to ten-times greater affinity for A147T. These results should inform the future design of compounds that bind both A147T and WT TSPO for use in neuroinflammation imaging

Effects of combined rice flour and molasses use on the growth performance of Pacific white shrimp (<em>Litopenaeus vannamei</em> Boone, 1931) applied biofloc technology

A 63-day completely random experiment with three replications was carried out to compare the effects of five different combination ratios of rice flour (R) and molasses (M) on the growth and survival rates of Pacific white shrimp (Litopenaeus vannamei Boone, 1931) postlarvae applied biofloc technology. Five biofloc (BF) treatments, including R90-M10, R70-M30, R50-M50, R30-M70, and R10-M90, formed with the addition of different combination ratios of rice flour and molasses, i.e., 90% R+10% M, 70% R+30% M, 50% R+50% M, 30% R+70% M, and 10% R+90% M, respectively, with C/N ratios of 15:1, and a control (neither rice flour nor molasses applied) was randomly arranged into the 18 plastic tanks of 1.0 m3 volume (with 0.5 m3 of water) each tank and salinity of 15‰. The postlarvae (0.095 g) were stocked into the tanks at a 150 ind. m−3 density and fed pelleted feed (40% protein). There was an improvement in growth (FMW, WG, DWG, and SGR) for all treatments. Besides, treatments with more than or equal to 30% molasses have improved SR, FCR, and FB. Especially the highest SR (94.2%) was obtained at the R70-M30, which perhaps created the highest FB (1.435 kg m−3) in this treatment. The lowest FCR (1.28) was also observed in the R70-M30 and significantly differed from the control and other treatments. Besides, water quality parameters were within the ranges recommended for Pacific white shrimp health during the experimental period. Our findings indicated the benefits of shrimp culture using the BF system when different combined ratios of rice flour and molasses were applied, of which a ratio of 70% rice flour and 30% molasses was considered as the best

Factors affecting the decision to choose a university of high school students: A study in An Giang Province, Vietnam

It is important to provide high school students with the necessary information for them to consult and make a decision to choose a university. The study aims to identify and evaluate the influence of factors in the decision to choose a university for high school students. The questionnaire survey method was used to collect data from 393 students from eight high schools in An Giang Province, Vietnam. Exploratory factor analysis and linear regression were used to analyze the data. The research results show that students are quite satisfied and quite certain with their decision to choose a university, while there are six important factors affecting the decision to choose a university. Influential factors with decreasing order of magnitude are: i) Factors consulted by teachers, family, friends, and relatives; ii) Factors of future job opportunities; iii) Factors of media activities; iv) Factors of learning conditions; v) Factors of university reputation; vi) Factors belong to the students themselves. The findings of the study show that there is no statistically significant difference between the group of males and females, between grades 10, 11, and 12. Besides, there is a statistically significant difference between students in high schools. The findings of this study have theoretical and practical implications for university admissions in Vietnam. Proposals made to university administrators were discussed. From the research results, we want to help students find the right university, and support universities to improve the efficiency of admissions

MARITIME SECURITY POLICY OF INDIA IN EARLY 21ST CENTURY: VIETNAM’S PERCEPTION OF ITS IMPLICATION ON THE ASIA-PACIFIC REGION

Since the early 21st century, the Asia-Pacific has become a dynamic region of development by some powerful countries in the world such as the United States (US), India, China, and Russia. Thus, the issue of ensuring maritime security to develop sea trade plays a central role in the strategies of these countries. From India’s perspective, maritime security in the Indian Ocean – Pacific Ocean is a deciding factor in the development, affirming its position and creating a balance of power in the country in comparison with other countries in the region. Nevertheless, the developed sea trade of India has faced challenges from various countries including the US, and China. Therefore, India has promoted a cooperative relationship with Vietnam to guarantee maritime security for Indian traders in the region. This paper aims to provide general information about maritime security as well as to determine and estimate India’s maritime security strategies. Additionally, it will present the role of Vietnam in India’s maritime security policies. The findings show that both nations, India and Vietnam have adequate backup strategies, which is the foundation for developing sea trade sustainability. Furthermore, India and Vietnam will play an increasingly strong role in the Asia-Pacific in the future.

Straightforward Procedure for Laboratory Production of DNA Ladder

DNA ladder is commonly used to determine the size of DNA fragments by electrophoresis in routine molecular biology laboratories. In this study, we report a new procedure to prepare a DNA ladder that consists of 10 fragments from 100 to 1000 bp. This protocol is a combination of routinely employed methods: cloning, PCR, and partial digestion with restriction enzymes. DNA fragments of 100 bp with unique restriction site at both ends were self-ligated to create a tandem repeat. Once being cloned, the tandem repeat was rapidly amplified by PCR and partially digested by restriction enzymes to produce a ladder containing multimers of the repeated DNA fragments. Our procedure for production of DNA ladder could be simple, time saving, and inexpensive in comparison with current ones widely used in most laboratories

Nanomaterial for Adjuvants Vaccine: Practical Applications and Prospects

Vaccines contain adjuvants to strengthen the immune responses of the receiver against pathogen infection or malignancy. A new generation of adjuvants is being developed to give more robust antigen-specific responses, specific types of immune responses, and a high margin of safety. By changing the physical and chemical properties of nanomaterials, it is possible to make antigen-delivery systems with high bioavailability, controlled and sustained release patterns, and the ability to target and image. Nanomaterials can modulate the immune system so that cellular and humoral immune responses more closely resemble those desired. The use of nanoparticles as adjuvants is believed to significantly improve the immunological outcomes of vaccination because of the combination of their immunomodulatory and delivery effects. In this review, we discuss the recent developments in new adjuvants using nanomaterials. Based on three main vaccines, the subunit, DNA, and RNA vaccines, the possible ways that nanomaterials change the immune responses caused by vaccines, such as a charge on the surface or a change to the surface, and how they affect the immunological results have been studied. This study aims to provide succinct information on the use of nanomaterials for COVID-19 vaccines and possible new applications

Comparative study on vision based rice seed varieties identification

Abstract-This paper presents a system for automated classification of rice variety for rice seed production using computer vision and image processing techniques. Rice seeds of different varieties are visually very similar in color, shape and texture that make the classification of rice seed varieties at high accuracy challenging. We investigated various feature extraction techniques for efficient rice seed image representation. We analyzed the performance of powerful classifiers on the extracted features for finding the robust one. Images of six different rice seed varieties in northern Vietnam were acquired and analyzed. Our experiments have demonstrated that the average accuracy of our classification system can reach 90.54% using Random Forest method with a simple feature extraction technique. This result can be used for developing a computer-aided machine vision system for automated assessment of rice seeds purity

Imaging cytoplasmic cAMP in mouse brainstem neurons

<p>Abstract</p> <p>Background</p> <p>cAMP is an ubiquitous second messenger mediating various neuronal functions, often as a consequence of increased intracellular Ca²⁺levels. While imaging of calcium is commonly used in neuroscience applications, probing for cAMP levels has not yet been performed in living vertebrate neuronal tissue before.</p> <p>Results</p> <p>Using a strictly neuron-restricted promoter we virally transduced neurons in the organotypic brainstem slices which contained pre-Bötzinger complex, constituting the rhythm-generating part of the respiratory network. Fluorescent cAMP sensor Epac1-camps was expressed both in neuronal cell bodies and neurites, allowing us to measure intracellular distribution of cAMP, its absolute levels and time-dependent changes in response to physiological stimuli. We recorded [cAMP]_ichanges in the micromolar range after modulation of adenylate cyclase, inhibition of phosphodiesterase and activation of G-protein-coupled metabotropic receptors. [cAMP]_ilevels increased after membrane depolarisation and release of Ca²⁺from internal stores. The effects developed slowly and reached their maximum after transient [Ca²⁺]_ielevations subsided. Ca²⁺-dependent [cAMP]_itransients were suppressed after blockade of adenylate cyclase with 0.1 mM adenylate cyclase inhibitor 2'5'-dideoxyadenosine and potentiated after inhibiting phosphodiesterase with isobutylmethylxanthine and rolipram. During paired stimulations, the second depolarisation and Ca²⁺release evoked bigger cAMP responses. These effects were abolished after inhibition of protein kinase A with H-89 pointing to the important role of phosphorylation of calcium channels in the potentiation of [cAMP]_itransients.</p> <p>Conclusion</p> <p>We constructed and characterized a neuron-specific cAMP probe based on Epac1-camps. Using viral gene transfer we showed its efficient expression in organotypic brainstem preparations. Strong fluorescence, resistance to photobleaching and possibility of direct estimation of [cAMP] levels using dual wavelength measurements make the probe useful in studies of neurons and the mechanisms of their plasticity. Epac1-camps was applied to examine the crosstalk between Ca²⁺and cAMP signalling and revealed a synergism of actions of these two second messengers.</p