Kinetic characteristics of biochemical parameters during consupren therapy after allogenic transplantation of the kidney

Changes in biochemical and common clinical parameters of the blood were detected in patients treated with consupren (cyclosporin) as the main immunosuppressant after allogenic transplantation of the kidney. Kinetic model for evaluation of treatment adequacy was based on therapeutic drug monitoring and monitoring of blood biochemistry in recipients of the primary kidney transplant. The k1, t1/2, C0, clearance (for hemoglobin), conditioned volume of distribution (for cyclosporin) of processes of biochemical parameters (toxicity markers) stabilization within the framework of a single-part kinetic model were determined. © Springer Science+Business Media, Inc. 2004

Kinetic characteristics of biochemical parameters during consupren therapy after allogenic transplantation of the kidney

Changes in biochemical and common clinical parameters of the blood were detected in patients treated with consupren (cyclosporin) as the main immunosuppressant after allogenic transplantation of the kidney. Kinetic model for evaluation of treatment adequacy was based on therapeutic drug monitoring and monitoring of blood biochemistry in recipients of the primary kidney transplant. The k1, t1/2, C0, clearance (for hemoglobin), conditioned volume of distribution (for cyclosporin) of processes of biochemical parameters (toxicity markers) stabilization within the framework of a single-part kinetic model were determined. © Springer Science+Business Media, Inc. 2004

Quantitative estimation of gemcitabine by HPLC in plasma

A method for estimating the antitumor agent gemcitabine in plasma by isocratic HPLC was developed. Sample preparation consisted of precipitating proteins with a mixture of acetonitrile and methanol (9:1) with subsequent acidification of the extract with glacial acetic acid. Supernatants were then evaporated to dryness in a rotary vacuum evaporator at 42°C. The dry residue was dissolved in the mobile phase, which consisted of a mixture of acetate buffer pH 5.0 and acetonitrile (97.5:2.5). UV detection of gemcitabine was performed at 282 nm. The detection limit for this compound was 1 μg/ml. This method can be used to study the pharmacokinetic characteristics of the agent within the frameworks of the protocol "Studies of dose-dependent regimes of long-term infusions of gemcitabine in patients with local and disseminated pancreas cancer". © 2008 Springer Science+Business Media, Inc

Quantitative estimation of gemcitabine by HPLC in plasma

A method for estimating the antitumor agent gemcitabine in plasma by isocratic HPLC was developed. Sample preparation consisted of precipitating proteins with a mixture of acetonitrile and methanol (9:1) with subsequent acidification of the extract with glacial acetic acid. Supernatants were then evaporated to dryness in a rotary vacuum evaporator at 42°C. The dry residue was dissolved in the mobile phase, which consisted of a mixture of acetate buffer pH 5.0 and acetonitrile (97.5:2.5). UV detection of gemcitabine was performed at 282 nm. The detection limit for this compound was 1 μg/ml. This method can be used to study the pharmacokinetic characteristics of the agent within the frameworks of the protocol "Studies of dose-dependent regimes of long-term infusions of gemcitabine in patients with local and disseminated pancreas cancer". © 2008 Springer Science+Business Media, Inc