8 research outputs found
Adhesion-induced phase separation of multiple species of membrane junctions
A theory is presented for the membrane junction separation induced by the
adhesion between two biomimetic membranes that contain two different types of
anchored junctions (receptor/ligand complexes). The analysis shows that several
mechanisms contribute to the membrane junction separation. These mechanisms
include (i) the height difference between type-1 and type-2 junctions is the
main factor which drives the junction separation, (ii) when type-1 and type-2
junctions have different rigidities against stretch and compression, the
``softer'' junctions are the ``favored'' species, and the aggregation of the
softer junction can occur, (iii) the elasticity of the membranes mediates a
non-local interaction between the junctions, (iv) the thermally activated shape
fluctuations of the membranes also contribute to the junction separation by
inducing another non-local interaction between the junctions and renormalizing
the binding energy of the junctions. The combined effect of these mechanisms is
that when junction separation occurs, the system separates into two domains
with different relative and total junction densities.Comment: 23 pages, 6 figure
Dynamic phase separation of fluid membranes with rigid inclusions
Membrane shape fluctuations induce attractive interactions between rigid
inclusions. Previous analytical studies showed that the fluctuation-induced
pair interactions are rather small compared to thermal energies, but also that
multi-body interactions cannot be neglected. In this article, it is shown
numerically that shape fluctuations indeed lead to the dynamic separation of
the membrane into phases with different inclusion concentrations. The tendency
of lateral phase separation strongly increases with the inclusion size. Large
inclusions aggregate at very small inclusion concentrations and for relatively
small values of the inclusions' elastic modulus.Comment: 6 pages, 6 figure
Physical model for the gating mechanism of ionic channels
We propose a physical model for the gating mechanism of ionic channels. First, we investigate the fluctuation-mediated interactions between two proteins imbedded in a cellular membrane and find that the interaction depends on their orientational configuration as well as the distance between them. The orientational dependence of interactions arises from the fact that the noncircular cross-sectional shapes of individual proteins constrain fluctuations of the membrane differently according to their orientational configuration. Then, we apply these interactions to ionic channels composed of four, five, and six proteins. As the gating stimulus creates the changes in the structural shape of proteins composing ionic channels, the orientational configuration of the ionic channels changes due to the free energy minimization, and ionic channels are open or closed according to the conformation thereof.open3
Two direct methods to calculate fluctuation forces between rigid objects embedded in fluid membranes
Predicting experimental quantities in protein folding kinetics using stochastic roadmap simulation
10.1007/11732990_34Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)3909 LNBI410-42