Evaluation of developmental competence of Sus scrofa domesticus (L.) oocyte-cumulus complexes after intra- and extraovarian vitrification

The aim of the present study was to identify the influence of extra- (EOV) and intraovarian vitrification (IOV) on mitochondrial activity (MA) and chromatin state in porcine oocytes during maturation in vitro. During EOV porcine oocytes were exposed in cryoprotective solutions (CPS): CPS-1 – 0.7 M dimethyl sulfoxide (DMSO)+0.9 M ethylene glycol (EG); CPS-2 – 1.4 M DMSO+1.8 M EG; CPS-3 – 2.8 M DMSO+3.6 M EG+0.65 M trehalose. At IOV the ovarian fragments were exposed in CPS-1 – 7.5 % EG+7.5 % DMSO, then in CPS-2 – 15 % EG, 15 % DMSO and 0.5 M sucrose. Straws with oocytes and ovarian fragments were plunged into LN2 and stored. For devitrification, the EOV oocytes were washed in solutions of 0.25, 0.19 and 0.125 M of trehalose, the IOV – in 0.5 and 0.25 М trehalose. Oocytes were cultured in NCSU-23 medium with 10 % fluid of follicles, follicular walls, hormones. 0.001 % of highly dispersed silica nanoparticles (ICP named after A.A. Chuyko of the NAS of Ukraine) were added to all media. The methods of fertilization and embryo culture are presented in the guidelines developed by us. MA and chromatin state were measured by MitoTracker Orange CMTMRos and the cytogenetic method. Significant differences in the level of oocytes with high-expanded cumulus between control and experimental vitrified groups (81 % versus 59 % and 52 %, respectively, p ≤ 0.001) were observed. The percentage of pyknotic cells in native oocytes was 19 %, EOV or IOV oocytes were 39 % and 49 %, respectively. After culture, the level of matured native oocytes was 86 %, 48 % EOV and 33 % IOV cells finished the maturation (p ≤ 0.001). Differences were also observed in the level of MA between groups treated by EOV and IOV (89.4±7.5 µA and 149.2±11.3 µA, respectively, p ≤ 0.05). For the first time, pre-implantation embryos were obtained from oocytes treated by IOV

The role of highly dispersed silica nanoparticles in the realization of the effects of granulosa on the maturation and fertilization competence of <i>Sus scrofa domesticus</i> oocytes

Reproductive technologies are some of the key directions in the context of the need to preserve and select highly productive farmed animals in terms of economically useful traits. Improvements of the existing models of the in vitro oocyte maturation system help to solve the problem of low yield of porcine embryos at the final stages of preimplantation development. In the present study, a model of culture medium for gametes (NCSU-23 with 10 % homologous follicular fluid, 10 IU hCG and 10 IU eCG) modernized by the addition of 1·106 granulosa cells (GCs) per ml and 0.001 % of highly dispersed silica nanoparticles (HDSn) is proposed for use in the IVM and IVF technology of donor porcine oocytes. Analysis of the oocyte chromatin status by the Tarkowsky method and assessment of the level of destructive changes in chromatin (apoptosis, pyknosis) revealed a significant percentage increase in matured oocytes and a decrease in the proportion of granulosa cells with degenerated chromatin when using the original culture system. The positive effects of a joint addition of GCs and HDSn to the maturation system have made it possible to increase the indicators of the meiotic maturation and fertilization of oocytes. Optimal results of developmental competence of oocytes were achieved with the joint use of GCs and HDSn in the maturation system (the proportion of matured cells reached 89 %, the level of oocytes with chromosome degeneration was 12 %, 39 % of embryos reached the final stage of preimplantation development). The positive effect of HDSn on oocyte fertilization was accompanied by an abrupt decrease in destructive processes in GCs during culture in the presence of HDSn. The level of somatic cells with pyknotic nuclei was 32 % and the level of apoptosis (TUNEL-test), 21 %, compared with the control (43 and 31 %, p &lt;0.01, respectively). Thus, a high efficiency of the porcine oocyte maturation system in the joint culture of gametes with GCs and HDSn was revealed. It makes it possible to recommend a model of this culture medium at the IVM of female gametes of Sus scrofa domesticus for improving the quality of donor oocytes used in cell and genetic engineering

U-Pb LA-ICP-MS DATING OF DETRITAL ZIRCON FROM UPPER PERMIAN SANDSTONE OF THE BOYEVAYA GORA SECTION (ORENBURG REGION, SOUTHERN CIS-URALS)

The first results of U-Pb LA-ICP-MS dating of 150 detrital zircon grains from the Upper Permian sandstone of the Boyevaya Gora section (Orenburg region, the Southern Cis-Urals) are presented. 95 conditional zircon U-Pb isotopic dates form two age peaks – 381 and 529 Ma. It is assumed that clastic material entered the sedimentation basin mainly due to erosion of the Early Hercynian Ural complexes and relics of the Protouralian-Timanian orogen

Features of management of the women's team in the organizations of sphere of services

Целью настоящей стать является описание особенностей управления женским коллективом в организациях сферы услуг.The purpose of this be is to describe the features of the management of female staff in the organizations of sphere of services

The development of a new bread wheat genotype carrying two loci for endosperm softness

The technological purpose of grain and flour wheat is largely determined by the grains endosperm structure. Its variability among wheat varieties depends mainly on the multiple allelism for a single Ha locus on chromosome 5D leading to a continuous variation of the trait. The grain endosperm can vary from hard and vitreous suitable for yeast baking to soft and floury favorable for confectionery and technical purposes. Furthermore, these traits, especially vitreousness, are strongly influenced by the growth conditions. Earlier, the Ha-Sp locus was introgessed into chromosome 5A of the bread wheat line 84/98w from Aegilops speltoides Tausch., which reduces endosperm hardness and vitreousness, like the dominant allele of the Ha locus. This paper is the first to describe the obtaining and testing of the supersoft lines combining in their genotype the homoeoallelic loci Ha-Sp of the line 84/98w and Ha of the soft grain cultivar Chinese Spring. The lines were isolated from 6–8 generations of self-pollinated F2 hybrids. They consistently exhibit a greater grain softness than the parental forms under both greenhouse and field conditions. These lines can be used in the breeding of wheat cultivars, the flour of which will not require chemical baking powder in the confectionery industry. It is also possible to use them for technical purposes for the production of bioethanol. In addition, these lines may serve as a genetic model for the study of the functional activity of homoeoallelic genes in the complex polyploid genomes of plants

Experience of the successful treatment with canakinumab of a patient with NLPC4-associated autoinflammatory syndrome with enterocolitis

The article shows the observation of rare NLPC4-associated autoinflammatory syndrome with enterocolitis and familial cold urticaria. Diagnosis is confirmed molecularly-genetically: previously not described mutation c.928C&gt;T in the heterozygous state in NLRC4 gene is discovered by a method of the new generation sequencing. The use of a monoclonal antibody to the interleukin 1 canakinumab provided complete relief of fever and skin and intestinal symptoms in just 1 week of treatment. Later the signs of inflammation have disappeared completely; the patient’s quality of life improved and life-threatening complications were prevented. The above example demonstrates the high clinical efficacy of canakinumab in the patient with NLRC4-associated autoinflammatory syndrome and suggests promising therapeutic use of interleukin 1 blockers in such patients. There were no adverse events during canakinumab therapy

High Efficiency of Kanakinumabum for a Patient with a Late Diagnosed Chronic Infantile Neurological Cutaneous Articular Syndrome (CINCA)

The article presents the monitoring of a severe course of CINCA/NOMID syndrome diagnosed at late stages. The use of monoclonal antibodies to IL 1 — kanakinumabum — in a patient with chronic neurological cutaneous and articular syndrome was successful: fever, cutaneous and pain syndromes were completely stopped, joint contractures decreased after one week of therapy. Laboratory parameters of the child’s disease activity (ESR and CRP) became normal after 8 weeks of treatment. Movements in the affected joints recovered completely after 24 weeks; the audiologist noted an improvement in hearing. The above clinical example demonstrates the high efficiency of kanakinumabum for a patient with chronic neurological cutaneous and articular syndrome, and shows the perspective of therapeutic application of IL 1 blocker for patients with CINCA syndrome including advanced stages of the disease. No adverse effects were noted during kanakinumabum therapy

The application of the «Lavomax» preparation as a part of complex therapy at rehabilitation of women with infringements of reproductive function on the pregravid stage in comparison with standard therapy

The investigation of 60 women of reproductive age at a pregravid preparations stage is carried for the purpose of an estimation of efficiency of therapy by a preparation «Lavomax» as a part of complex therapy for rehabilitation of women with infringement of reproductive function in comparison with efficiency of standard therapy. Convincing proofs of clinical efficiency and shipping of a «Lavomax» preparation are received, that allows to recommend it in clinical practice in complex treatment of sexual-transmissie infections.Проведено обследование 60 женщин репродуктивного возраста на этапе предгравидарной подготовки с целью оценки эффективности терапии препаратом «Лавомакс» в составе комплексной терапии для реабилитации женщин с нарушением репродуктивной функции в сравнении с эффективностью стандартной терапии. Получены убедительные доказательства клинической эффективности и переносимости препарата «Лавомакс», что позволяет рекомендовать его в клинической практике в комплексном лечении секуально-трансмиссивных инфекций

ПЕРВЫЕ РЕЗУЛЬТАТЫ U-Pb LA-ICP-MS ДАТИРОВАНИЯ ОБЛОМОЧНОГО ЦИРКОНА ИЗ ВЕРХНЕПЕРМСКИХ ПЕСЧАНИКОВ РАЗРЕЗА БОЕВАЯ ГОРА (ОРЕНБУРГСКАЯ ОБЛАСТЬ, ЮЖНОЕ ПРЕДУРАЛЬЕ)

The first results of U-Pb LA-ICP-MS dating of 150 detrital zircon grains from the Upper Permian sandstone of the Boyevaya Gora section (Orenburg region, the Southern Cis-Urals) are presented. 95 conditional zircon U-Pb isotopic dates form two age peaks – 381 and 529 Ma. It is assumed that clastic material entered the sedimentation basin mainly due to erosion of the Early Hercynian Ural complexes and relics of the Protouralian-Timanian orogen. Представлены первые результаты U-Pb LA-ICP-MS датирования 150 зерен обломочного циркона из песчаников терминальной перми разреза Боевая Гора (Оренбургская область, Южное Предуралье). В полученном по 95 кондиционным оценкам изотопного возраста распределении отчетливо доминирует палеозойская популяция с двумя максимумами – 381 и 529 млн лет. Предполагается, что обломочный материал поступал в бассейн осадконакопления главным образом за счет эрозии как раннегерцинских комплексов Уральского орогена, так и реликтов Протоуральско-Тиманского складчатого сооружения.