Free Radical Oxidation and its Part in Hypoxia Adaptation in Epistaxis

Purpose: A comparative study of free radical oxidation in the blood of those suffering from single and recurrent epistaxis.Materials and Methods: In 98 patients malon dialdegid, superoxiddismutase and total peroxidase activity of blood red cells were examined.Results: Recurrent epistaxis is shown to be followed by a deteriorated content of red blood cells and haemoglobin, which may result in hypoxia. In this group, FRO takes part in hypoxia adaptation: increased superoxide scavenger activity protects red cells from oxidative damage. Physical exercise tests have proved a low reserve of the anti-oxidant system in the blood of recurrent epistaxis patients.Summary: The state of the patient, suffering from recurrent epistaxis depends on free radical oxidation system reserves

THE CONTENT OF STEROID HORMONES IN PATIENTS WITH CHOLELITHIASIS, DEPENDING ON THE DISEASE

Here, in this work, the peculiarities of secretion of steroid hormones have been researched in patients with gallstone disease depending on the course of the disease. It is suggested, that the lack of use of cholesterol for steroidogenesis leads to its precipitation in the composition of gallstones, increased cortisol is a limiting factor in inflammatory processes in the gall bladder. The increasing of testosterone synthesis may be considered as an antagonistic component of glucocorticoid regulation of lipid and protein metabolism

MOLECULES OF MEDIUM MASS IN DEFINITION OF SAFETY MEAT AND FAT OF PIGS GROWN WITH THE USE OF ANTIBACTERIAL DRUGS

Purpose: to determine the amount of medium mass molecules (MMM) in the blood of rats, weaned receiving rations of meat and fat pigs, grown with the use of complex antibacterial drugs (Tylosin, Olaquindox and Zinkbazitrazin).Materials and Methods: this investigation was conducted during 30 days on 48 weaned rats. The level of MMM was determined spectrophotometrically at a wavelength (λ) 254 and 280 nm.Results: it was revealed that the MMM level was higher in animals received given in the diet of swine meat and fat in their ration grown with the use of antibacterial drugs than in control group. The lowering of MMM coefficient with the introduction of pigs fat in the diet of rats is evidenced about the homeostasis system strain.Summary: determination of the level of MMM in the blood serum is effective method of assessing in the food staffs safety

The changes of the tubular epithelium phenotype in the contralateral kidney nephrons while developing unilateral ureteral obstruction: an experimental study

Introduction. The high prevalence of renal diseases caused by urinary tract obstruction led to the need for experimental research of compensatory and pathological processes with kidney injury. It is also of relevance to study key mechanisms providing a compensatory function of the contralateral kidney for early diagnosis, treatment, and prognosis of obstructive renal diseases.Purpose of the study. To examine epithelial nephron cells phenotype dynamics changes in contralateral kidney using unilateral ureteral obstruction experimental model.Materials and methods. Model of unilateral ureteral obstruction was established using adult rabbits. The studies were carried out on days 7, 14 and 21 of complete obstruction of the left ureter. Immunophenotyping was performed on contralateral kidney tissue samples using epithelial (cytokeratin 7, E-cadherin) and mesenchymal (vimentin, α-smooth muscle actin) markers.Results. The contralateral kidney under additional load can maintain the morphological and functional characteristics of the nephron for a long time. The first transmogrify signs in the nephron epithelium phenotype were detected by day 21 as the diffuse appearance of mesenchymal marker vimentin with unaltered visualization of epithelial phenotype markers.Conclusion. The results obtained allow us to assume that the compensatory reserve of the contralateral kidney is gradually decreasing when the duration of the obstruction increases. Thus, the likelihood of developing negative disorders increases

SEROEPIDEMIOLOGICAL RESEARCH AND ANALYSIS CHARACTERISTIC OF KEFIR, AS A VALUABLE PROBIOTIC PRODUCT AND ITS BIOLOGICAL PROPERTIES

We present new data on the on the microbial composition and properties of probiotic kefir fungi fermented milk drink kefir, namely antikancerogenik, antimutagenic, antigenotoxic and radio-protecting, antioxidant, antihypertensive, hypoglycemic, anti-inflammatory, in wound healing, antimicrobial, and preventable and antiallergic biological effects

GUT MICROBIOTA AND HYGIENE THEORY OF ATOPY

The indigenous gut microbiota plays a key role in maintaining homeostasis in gastrointestinal tract. The review is devoted to factors influencing gut microbiota composition, involvement of microbiota in formation of systemic and mucosal immunity, the role of disturbances of microbial community in development of allergic disease

Dynamics of hematological blood parameters of experimental animals with unilateral urinary tract obstruction

The aim of the study – to investigate the dynamics of hematological blood parameters in case of OMI in the experiment.Цель исследования – изучение динамики гематологических показателей крови при ОНМ в эксперименте

Associative connection of infectious and inflammatory diseases in pregnancy and severe preeclampsia

Materials and methods. This retrospective case-control study enrolled 50 women with severe preeclampsia and 50 control women with spontaneous singleton pregnancy. Median age of women ranged from 20 to 35 years. All women did not have a history of hypertension, autoimmune, metabolic, renal, or cardiac diseases, and preeclampsia before this pregnancy. We have analyzed χ2, odds ratio (OR) and its 95% confidence intervals (95% Cl). Results. We found significant association between maternal systemic infectious and severe preeclampsia (OR = 49.6; 95% Cl 13.05-188.64). The risk of severe preeclampsia were significantly lower in patients with local infections of the lower genital tract (OR = 4.5; 95% Cl 1.49-6.71). Asymptomatic bacteriuria is associated with the highest risk of severe preeclampsia (OR = 17.0; 95% Cl 4.66-61.81). Acute gravidarum pyelonephritis showed lower association with severe preeclampsia (OR = 5.4; 95% Cl 1.69-10.54). We did not observe increased risk of severe preeclampsia with acute respiratory infections (OR = 2.0; 95% Cl 0.71-4.69). Acute non-specific bacterial vaginitis and acute candidiasis vulvovaginitis were found to be risk factors of severe preeclampsia (OR = 6.7; 95% Cl 1.90-11.02 and OR = 4.3; 95% Cl 1.45-9.99 respectively). Cytomegalovirus infection (2 %), toxoplasmosis (2 %), Chlamydia trachomatis cervicitis (4 %), acute Trichomonas colpitis (2 %) and bacterial vaginosis (4 %) were found only in patients with severe preeclampsia. Conclusion. Our data support that acute maternal infection is associated with an increased risk of severe preeclampsia in healthy women with singleton pregnancy. Systemic inflammatory response might be the main potential mechanisms related to infections and enhanced development of severe preeclampsia. Further research is required to elucidate the underlying mechanism of this association

Disturbance of meromixis in saline Lake Shira (Siberia, Russia): possible reasons and ecosystem response

Saline Lake Shira (Southern Siberia, Russia) was meromictic through the observation period 2002-2015. During the under-ice periods of 2015 and 2016, complete mixing of the water column was recorded for the first time, and hydrogen sulphide temporarily disappeared from the water column of the lake; i.e. in those years the lake turned to holomixis. In the summer of 2015, a sharp increase in chlorophyll a, organic carbon, zooplankton, and phytoflagellates was observed in the lake, which was probably due to the release of nutrients from the monimolimnion. Purple sulfur bacteria completely disappeared from the lake after the first mixing in 2015, and did not reappear despite the restoration of meromixis in 2017. Thus, it was demonstrated that purple sulfur bacteria are sensitive to the weakening of the stratification of Lake Shira. Based on the data of the seasonal monitoring of temperature and salinity profiles over the period 2002-2017, it was presumed that the main cause of deep mixing in 2015 was the weakening of the salinity gradient due to strong wind impact and early ice retreat in the spring of 2014. In addition, it was shown that in previous years a significant contribution to the maintenance of meromixis was made by an additional influx of fresh water, which caused a rise in the lake level in the period 2002-2007. Thus, we identified a relationship between the stratification regime of the lake and the change in its level, which provides valuable information both for the forecast of water quality and for reconstruction of the Holocene climate humidity in this region of Southern Siberia from the sediment cores of Lake Shira

Tracking down carbon inputs underground from an arid zone Australian calcrete.

Freshwater ecosystems play a key role in shaping the global carbon cycle and maintaining the ecological balance that sustains biodiversity worldwide. Surficial water bodies are often interconnected with groundwater, forming a physical continuum, and their interaction has been reported as a crucial driver for organic matter (OM) inputs in groundwater systems. However, despite the growing concerns related to increasing anthropogenic pressure and effects of global change to groundwater environments, our understanding of the dynamics regulating subterranean carbon flows is still sparse. We traced carbon composition and transformations in an arid zone calcrete aquifer using a novel multidisciplinary approach that combined isotopic analyses of dissolved organic carbon (DOC) and inorganic carbon (DIC) (δ13CDOC, δ13CDIC, 14CDOC and 14CDIC) with fluorescence spectroscopy (Chromophoric Dissolved OM (CDOM) characterisation) and metabarcoding analyses (taxonomic and functional genomics on bacterial 16S rRNA). To compare dynamics linked to potential aquifer recharge processes, water samples were collected from two boreholes under contrasting rainfall: low rainfall ((LR), dry season) and high rainfall ((HR), wet season). Our isotopic results indicate limited changes and dominance of modern terrestrial carbon in the upper part (northeast) of the bore field, but correlation between HR and increased old and 13C-enriched DOC in the lower area (southwest). CDOM results show a shift from terrestrially to microbially derived compounds after rainfall in the same lower field bore, which was also sampled for microbial genetics. Functional genomic results showed increased genes coding for degradative pathways-dominated by those related to aromatic compound metabolisms-during HR. Our results indicate that rainfall leads to different responses in different parts of the bore field, with an increase in old carbon sources and microbial processing in the lower part of the field. We hypothesise that this may be due to increasing salinity, either due to mobilisation of Cl- from the soil, or infiltration from the downstream salt lake during HR. This study is the first to use a multi-technique assessment using stable and radioactive isotopes together with functional genomics to probe the principal organic biogeochemical pathways regulating an arid zone calcrete system. Further investigations involving extensive sampling from diverse groundwater ecosystems will allow better understanding of the microbiological pathways sustaining the ecological functioning of subterranean biota