Reflection on multilayer mirrors beam profile and coherence properties

The main advantage of Bragg reflection from a multilayer mirror as a monochromator for hard X rays, is the higher photon flux density because of the larger spectral bandpass compared with crystal lattice reflection. The main disadvantage lies in the strong modulations of the reflected beam profile. This is a major issue for micro imaging applications, where multilayer based monochromators are frequently employed to deliver high photon flux density. A subject of particular interest is the origin of the beam profile modifications, namely the irregular stripe patterns, induced by the reflection on a multilayer. For multilayer coatings in general it is known that the substrate and its surface quality significantly influence the performance of mirrors, as the coating reproduces to a certain degree the roughness and shape of the substrate. This proceedings article reviews recent experiments that indicate potential options for producing wave front preserving multilayer mirrors, as well as new details on the particular mirrors our group has extensively studied in the pas

Synchrotron-based radioscopy employing spatio-temporal micro-resolution for studying fast phenomena in liquid metal foams

High-speed synchrotron-based radioscopy is applied to study a coalescence event (which lasts ∼2 ms) in situ in a liquid metal foam

In vitro synchrotron-based radiography of micro-gap formation at the implant–abutment interface of two-piece dental implants

Micro-radiography using hard X-ray synchrotron radiation is the first potential tool to allow an evaluation of the mechanical behavior of the dental implant–abutment complex during force application, thus enabling the enhancement of the design of dental implants which has been based on theoretical analysis to date

Prognostic relevance of disseminated tumor cells in the bone marrow and biological factors of 265 primary breast carcinomas

Introduction The prognostic significance of disseminated tumor cells in the bone marrow (DTC-BM) of breast cancer patients has been demonstrated in many studies. Yet, it is not clear which of the primary tumors' biological factors predict hematogenous dissemination. We therefore examined `tissue micro arrays' (TMAs) of 265 primary breast carcinomas from patients with known bone marrow ( BM) status for HER2, Topoisomerase IIa ( Top IIa), Ki 67, and p53. Methods BM analysis was performed by cytospin preparation and immunocytochemical staining for cytokeratin (CK). TMAs were examined by immunohistochemistry (IHC) for HER2, Top IIa, Ki 67 and p53, and fluorescence in situ hybridization ( FISH) for HER2. Results HER2 ( 2+/ 3+) was positive in 35/167 (21%) cases ( FISH 24.3%), Top IIa (> 10%) in 87/187 (46%), Ki 67 in 52/ 184 (28%) and p53 (> 5%) in 61/174 cases (34%). Of 265 patients, 68 (25.7%) showed DTC-BM with a median of 2/2 x 106 cells ( 1 to 1,500). None of the examined factors significantly predicted BM positivity. Significant correlation was seen between HER2 IHC and Top IIa ( p = 0.06), Ki 67 ( p = 0.031), and p53 ( p <.001). Top IIa correlated with Ki 67 and p53, and Ki 67 also with p53 ( p = 0.004). After a median follow-up of 60.5 months ( 7 to 255), the presence of DTC-BM showed prognostic relevance for overall survival ( p = 0.03), whereas HER2 ( IHC, p = 0.04; FISH, p = 0.03) and Ki 67 ( p = 0.04) correlated with disease free survival, and HER2 with distant disease free survival ( IHC, p = 0.06; FISH, p = 0.05). Discussion The congruence of the examined factors' expression rates indicates a causal line of suppressor, proliferation, and mitosis markers, and growth factor receptors. Hematogenous tumor cell spread seems to be an independent process. The examination of these factors on DTC-BM is the aim of ongoing research

Differential interferometric synthetic aperture radar for tide modelling in Antarctic ice-shelf grounding zones

Differential interferometric synthetic aperture radar (DInSAR) is an essential tool for detecting ice-sheet motion near Antarctica's oceanic margin. These space-borne measurements have been used extensively in the past to map the location and retreat of ice-shelf grounding lines as an indicator for the onset of marine ice-sheet instability and to calculate the mass balance of ice sheets and individual catchments. The main difficulty in interpreting DInSAR is that images originate from a combination of several SAR images and do not indicate instantaneous ice deflection at the times of satellite data acquisitions. Here, we combine the sub-centimetre accuracy and spatial benefits of DInSAR with the temporal benefits of tide models to infer the spatio-temporal dynamics of ice–ocean interaction during the times of satellite overpasses. We demonstrate the potential of this synergy with TerraSAR-X data from the almost-stagnant southern McMurdo Ice Shelf (SMIS). We then validate our algorithm with GPS data from the fast-flowing Darwin Glacier, draining the Antarctic Plateau through the Transantarctic Mountains into the Ross Sea. We are able to reconstruct DInSAR-derived vertical displacements to 7 mm mean absolute residual error and generally improve traditional tide-model output by up to 39 % from 10.8 to 6.7 cm RMSE against GPS data from areas where ice is in local hydrostatic equilibrium with the ocean and by up to 74 % from 21.4 to 5.6 cm RMSE against GPS data in feature-rich coastal areas where tide models have not been applicable before. Numerical modelling then reveals Young's modulus of E=1.0±0.56  GPa and an ice viscosity of ν=10±3.65  TPa s when finite-element simulations of tidal flexure are matched to 16 d of tiltmeter data, supporting the hypothesis that strain-dependent anisotropy may significantly decrease effective viscosity compared to isotropic polycrystalline ice on large spatial scales. Applications of our method include the following: refining coarsely gridded tide models to resolve small-scale features at the spatial resolution and vertical accuracy of SAR imagery, separating elastic and viscoelastic contributions in the satellite-derived flexure measurement, and gaining information about large-scale ice heterogeneity in Antarctic ice-shelf grounding zones, the missing key to improving current ice-sheet flow models. The reconstruction of the individual components forming DInSAR images has the potential to become a standard remote-sensing method in polar tide modelling. Unlocking the algorithm's full potential to answer multi-disciplinary research questions is desired and demands collaboration within the scientific community

Study of multilayer-reflected beam profiles and their coherence properties using beamlines ID19 (ESRF) and 32-ID (APS)

Abstract. The use of multilayer mirrors is an interesting alternative for reflective X-ray monochromatization with respect to reflection from crystal optics. The increased photon flux density due to the multilayers&apos; larger bandwidth is of crucial importance for, e.g, full-field X-ray imaging applications. Drawbacks are the introduced modifications of the reflected beam profile as well as a certain loss of coherence, summarized as wavefront degradation. Our recent work has shown that the modification of the beam profile can vary with, e.g., the material composition of the coating applied. In order to verify our findings, a beamline round-robin has been initiated, comparing the wavefront profiles after reflection by selected multilayers at beamlines 32-ID (Advanced Photon Source) and ID19 (European Synchrotron Radiation Facility) with our initial results acquired at BM05 (ESRF

Femtosecond laser preparation of resin embedded samples for correlative microscopy workflows in life sciences

Correlative multimodal imaging is a useful approach to investigate complex structural relations in life sciences across multiple scales. For these experiments, sample preparation workflows that are compatible with multiple imaging techniques must be established. In one such implementation, a fluorescently labeled region of interest in a biological soft tissue sample can be imaged with light microscopy before staining the specimen with heavy metals, enabling follow-up higher resolution structural imaging at the targeted location, bringing context where it is required. Alternatively, or in addition to fluorescence imaging, other microscopy methods, such as synchrotron x-ray computed tomography with propagation-based phase contrast or serial blockface scanning electron microscopy, might also be applied. When combining imaging techniques across scales, it is common that a volumetric region of interest (ROI) needs to be carved from the total sample volume before high resolution imaging with a subsequent technique can be performed. In these situations, the overall success of the correlative workflow depends on the precise targeting of the ROI and the trimming of the sample down to a suitable dimension and geometry for downstream imaging. Here, we showcase the utility of a femtosecond laser (fs laser) device to prepare microscopic samples (1) of an optimized geometry for synchrotron x-ray tomography as well as (2) for volume electron microscopy applications and compatible with correlative multimodal imaging workflows that link both imaging modalities