Hsc66 substrate specificity is directed toward a discrete region of the iron-sulfur cluster template protein IscU

Hsc66 and Hsc20 comprise a specialized chaperone system important for the assembly of iron-sulfur clusters in Escherchia coli. Only a single substrate, the Fe/S template protein IscU, has been identified for the Hsc66/Hsc20 system, but the mechanism by which Hsc66 selectively binds IscU is unknown. We have investigated Hsc66 substrate specificity using phage display and a peptide array of IscU. Screening of a heptameric peptide phage display library revealed that Hsc66 prefers peptides with a centrally located Pro-Pro motif. Using a cellulose-bound peptide array of IscU we determined that Hsc66 interacts specifically with a region (residues 99-103, LPPVK) that is invariant among all IscU family members. A synthetic peptide (ELPPVKIHC) corresponding to IscU residues 98-106 behaves in a similar manner to native IscU, stimulating the ATPase activity of Hsc66 with similar affinity as IscU, preventing Hsc66 suppression of bovine rhodanese aggregation, and interacting with the peptide-binding domain of Hsc66. Unlike native IscU, however, the synthetic peptide is not bound by Hsc20 and does not synergistically stimulate Hsc66 ATPase activity with Hsc20. Our results indicate that Hsc66 and Hsc20 recognize distinct regions of IscU and further suggest that Hsc66 will not bind LPPVK motifs with high affinity in vivo unless they are in the context of native IscU and can be directed to Hsc66 by Hsc20

Observation of charged nanograins at comet 67P/Churyumov‐Gerasimenko

Soon after the Rosetta Orbiter rendezvoused with comet 67P/Churyumov‐Gerasimenko at a solar distance of ~3.5 AU and began to fly in triangular‐shaped trajectories around it, the Ion and Electron Sensor detected negative particles at energies from about 100 eV/q to over 18 keV/q. The lower energy particles came from roughly the direction of the comet; the higher‐energy particles came from approximately the solar direction. These particles are interpreted as clusters of molecules, most likely water, which we refer to as nanograins because their inferred diameters are less than 100 nm. Acceleration of the grains away from the comet is through gas drag by the expanding cometary atmosphere, while acceleration back to the vicinity of the comet is caused partly by solar radiation pressure but mainly by the solar wind electric field. These observations represent the first measurements of energetic charged submicron‐sized dust or ice grains (nanograins) in a cometary environment.Key PointsFirst observation of charged nanograins from a cometNanograins are reflected by solar radiation pressure and accelerated by solar wind electric fieldsNanograins return to the vicinity of the comet after significant energizationPeer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/113696/1/grl53313_am.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/113696/2/grl53313.pd

The Puzzle of the Flyby Anomaly

Close planetary flybys are frequently employed as a technique to place spacecraft on extreme solar system trajectories that would otherwise require much larger booster vehicles or may not even be feasible when relying solely on chemical propulsion. The theoretical description of the flybys, referred to as gravity assists, is well established. However, there seems to be a lack of understanding of the physical processes occurring during these dynamical events. Radio-metric tracking data received from a number of spacecraft that experienced an Earth gravity assist indicate the presence of an unexpected energy change that happened during the flyby and cannot be explained by the standard methods of modern astrodynamics. This puzzling behavior of several spacecraft has become known as the flyby anomaly. We present the summary of the recent anomalous observations and discuss possible ways to resolve this puzzle.Comment: 6 pages, 1 figure. Accepted for publication by Space Science Review

First results from the GPS atmosphere sounding experiment TOR aboard the TerraSAR-X satellite

GPS radio occultation events observed between 24 July and 17 November 2008 by the IGOR occultation receiver aboard the TerraSAR-X satellite are processed and analyzed. The comparison of 15 327 refractivity profiles with collocated ECMWF data yield a mean bias between zero and −0.30 % at altitudes between 5 and 30 km. Standard deviations decrease from about 1.4 % at 5 km to about 0.6 % at 10 km altitude, however, increase significantly in the upper stratosphere. At low latitudes mean biases and standard deviations are larger, in particular in the lower troposphere. The results are consistent with 15 159 refractivity observations collected during the same time period by the BlackJack receiver aboard GRACE-A and processed by GFZ's operational processing system. The main difference between the two occultation instruments is the implementation of open-loop signal tracking in the IGOR (TerraSAR-X) receiver which improves the tropospheric penetration depth in terms of ray height by about 2 km compared to the conventional closed-loop data acquired by BlackJack (GRACE-A)

Indication, from Pioneer 10/11, Galileo, and Ulysses Data, of an Apparent Anomalous, Weak, Long-Range Acceleration

Radio metric data from the Pioneer 10/11, Galileo, and Ulysses spacecraft indicate an apparent anomalous, constant, acceleration acting on the spacecraft with a magnitude $\sim 8.5\times 10^{-8}$ cm/s$^2$, directed towards the Sun. Two independent codes and physical strategies have been used to analyze the data. A number of potential causes have been ruled out. We discuss future kinematic tests and possible origins of the signal.Comment: Revtex, 4 pages and 1 figure. Minor changes for publicatio

On the possibility of measuring relativistic gravitational effects with a LAGEOS-LAGEOS II-OPTIS-mission

In this paper we wish to preliminary investigate if it would be possible to use the orbital data from the proposed OPTIS mission together with those from the existing geodetic passive SLR LAGEOS and LAGEOS II satellites in order to perform precise measurements of some general relativistic gravitoelectromagnetic effects, with particular emphasis on the Lense-Thirring effect.Comment: Abridged version. 16 pages, no figures, 1 table. First results from the GGM01C Earth gravity model. GRACE data include

Phenomenology of the Lense-Thirring effect in the Solar System

Recent years have seen increasing efforts to directly measure some aspects of the general relativistic gravitomagnetic interaction in several astronomical scenarios in the solar system. After briefly overviewing the concept of gravitomagnetism from a theoretical point of view, we review the performed or proposed attempts to detect the Lense-Thirring effect affecting the orbital motions of natural and artificial bodies in the gravitational fields of the Sun, Earth, Mars and Jupiter. In particular, we will focus on the evaluation of the impact of several sources of systematic uncertainties of dynamical origin to realistically elucidate the present and future perspectives in directly measuring such an elusive relativistic effect.Comment: LaTex, 51 pages, 14 figures, 22 tables. Invited review, to appear in Astrophysics and Space Science (ApSS). Some uncited references in the text now correctly quoted. One reference added. A footnote adde