63 research outputs found
Biosynthesis of heparin. Solubilization and partial purification of uridine diphosphate glucuronic acid, acceptor glucuronosyltransferase from mouse mastocytoma.
Galactosyltransfer in mouse mastocytoma, purification and properties of n-acetyllactosamine synthetase.
Glycosaminoglycan synthesis in mouse mastocytoma.
The glycosaminoglycan synthesis in Furth solid mastocytoma tissue has been studied. Approx. 10% of the polysaccharide isolated after incubation in vitro with [(14)C]-glucosamine was digestible with chondroitinase ABC and the product of digestion was identified as 2-acetamido-2-deoxy-3-O-(β-d-gluco-4-enepyranosyluronic acid)-4-O-sulpho-d-galactose. Similarly, labelling of polysaccharide in vivo with (35)SO(4)(2−) followed by isolation of mast-cell fractions by density-gradient centrifugation on colloidal silica revealed the presence of a polysaccharide which migrated as did chondroitin sulphate on electrophoresis in barium acetate. Chondroitinase ABC produced the same digestion product as before. Finally, the presence of the UDP-N-acetylgalactosamine–chondroitin 6-sulphate hexasaccharide N-acetylgalactosaminyltransferase previously implicated in chondroitin sulphate biosynthesis was demonstrated in microsomal particles from fractions of purified mast cells
Immunization of mice against tetanus with fragments of tetanus toxin synthesized in Escherichia coli
Cloning, nucleotide sequencing, and expression of tetanus toxin fragment C in Escherichia coli
Synthesis of a novel glycosaminoglycan pentasaccharide serine having anN-acetylgalactosamine residue?-linked to the core linkage tetrasaccharide
Limited proteolysis of tetanus toxin. Relation to activity and identification of cleavage sites
Two N-acetylgalactosaminyltransferase are involved in the biosynthesis of chondroitin sulfate
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