Quantification of trypsin with a radioimmunoassay in herring larvae (Clupea harengus) compared with a highly sensitive fluorescence technique to determine tryptic enzyme activity

Enzymatic activity and quantity of the protease trypsin were measured in individual herring larvae (Clupea harengus L.). The enzymatic activity assay was done using a fluorescence technique, and a radioimmunoassay was used for quantification of trypsin. The results are compared and the differences between the techniques discussed. Both methods gave similar results, as high or low values in trypsin quantity were reflected in high or low values of tryptic activity. Quantity and activity were linearly and positively correlated, but small differences between methods were found at the lowest detection limits. Both techniques reflect high variability between individual larvae

MÉCANISME DE LA SÉCRÉTION PANCRÉATIQUE EXOCRINE CHEZ LE PORC : RÉGULATION PAR RÉTROINHIBITION

International audienc

The adaptation of digestive enzymes to the diet : Its physiological significance

International audienc

SÉCRÉTION PANCRÉATIQUE SUR PORC FISTULÉ ADAPTATION A LA TENEUR EN PROTÉINES DU RÉGIME

International audienc

ÉVOLUTION DU SYSTÈME ENZYMATIQUE DU PANCRÉAS ET DE L'INTESTIN CHEZ LE PORCELET ALLAITÉ

International audienc

Effets à long terme de la ligature du canal pancréatique sur la digestibilité apparente chez le porc

International audienc

Effets de la modification de la composition du régime alimentaire sur les teneurs plasmatiques de quelques peptides de régulation de la sécrétion pancréatique chez le porc

Exterior, general vie

APPORT DE PROTÉINES D'ORIGINE ENDOGÈNE PAR LA SÉCRÉTION DU PANCRÉAS EXOCRINE CHEZ LE PORC

International audienc