Quality related communication approaches for organic food

As food quality becomes more complex, consumer s are tending to reduce their involvement in the food purchase decision- making process. Consequently, prices are becoming more significant as they represent an easy choice criterion when consumer s have less information about the differences between the product and process quality inherent in food items (including environmental or social issues). The organic food sector provides high, complex food quality profiles and has therefore been seriously affected by this development. Consequently some SMEs in the food business sector are starting to emphasize certain quality factors, such as sustainable production and processing, as a means of communicating their added value to consumer s. Based on published scientific and unpublished literature, this paper provides an overview of the main instrument s and media of communication on food quality, illustrated by case studies. It also present s the results of a test by means of an information display matrix, conducted in order to analyse the consumer information and quality assessment behaviour involved in apple purchase in Switzerland. Keywords food quality, food indicators, means of communication, food miles, social standards, consumer behaviour.Marketing,

Small coupling limit and multiple solutions to the Dirichlet Problem for Yang Mills connections in 4 dimensions - Part I

In this paper (Part I) and its sequels (Part II and Part III), we analyze the structure of the space of solutions to the epsilon-Dirichlet problem for the Yang-Mills equations on the 4-dimensional disk, for small values of the coupling constant epsilon. These are in one-to-one correspondence with solutions to the Dirichlet problem for the Yang Mills equations, for small boundary data. We prove the existence of multiple solutions, and, in particular, non minimal ones, and establish a Morse Theory for this non-compact variational problem. In part I, we describe the problem, state the main theorems and do the first part of the proof. This consists in transforming the problem into a finite dimensional problem, by seeking solutions that are approximated by the connected sum of a minimal solution with an instanton, plus a correction term due to the boundary. An auxiliary equation is introduced that allows us to solve the problem orthogonally to the tangent space to the space of approximate solutions. In Part II, the finite dimensional problem is solved via the Ljusternik-Schirelman theory, and the existence proofs are completed. In Part III, we prove that the space of gauge equivalence classes of Sobolev connections with prescribed boundary value is a smooth manifold, as well as some technical lemmas used in Part I. The methods employed still work when the 4-dimensional disk is replaced by a more general compact manifold with boundary, and SU(2) is replaced by any compact Lie group

G-genotyping of rotaviruses in stool samples in Salento, Italy

Rotaviruses are the most common agents of diarrhoeal illnessin infants and young children. Gastroenteritis caused byrotaviruses is also more likely to be associated with severe dehydration compared to other viral gastroenteritis.We determined the G-genotype of rotaviruses circulating in theSalento. During 2004, 144 stool samples were collected fromsubjects with a positive screening test and stored at -20 °C untilconfirmation could take place using molecular biology techniques. A reverse transcription-polymerase chain reaction (RTPCR) assay for the amplification of gene VP7 of rotavirus wasdeveloped and used; G-genotype was determined by sequencing and phylogenetic analysis. A total of 101 stool samples were detected positive by RT-PCRand 35 were sequence analyzed and classified into G1, G2, G4,G9 and G12 type. The overall relative incidence of G-types inSalento is different to that of other developed countries. Themost prevalent genotype was G2 while genotype G1 was present at low levels. It is also interesting to note the presence ofG9 rotavirus, which is now recognized as the fifth globallyimportant rotavirus genotype.In this study, therefore, we demonstrate the usefulness of a simple method for correctly determining the G genotypes circulating in a geographic region

Selenocarbamates As a Prodrug-Based Approach to Carbonic Anhydrase Inhibition

A study on the activity of selenocarbamates as a novel chemotype acting as carbonic anhydrase (CA, EC 4.2.1.1) inhibitors is reported. Undergoing CA‐mediated hydrolysis, selenocarbamates release selenolates behaving as zinc binding groups and effectively inhibiting CAs. A series of selenocarbamates characterised by high molecular diversity and complexity have been studied against different human CA isoforms such as hCA I, II, IX and XII. Selenocarbamates behave as masked selenols with potential biological applications as prodrugs for CAs inhibition‐based strategies. X‐ray studies provided insights into the binding mode of this novel class of CA inhibitors

Serological evidence of Toscana virus infection in Portuguese patients.

SUMMARYToscana virus (TOSV) is an emergingPhlebovirusof growing interest as a human pathogen in the Mediterranean Basin. In Portugal, however, little is known about the prevalence of TOSV infection. The aim of this work was to perform a seroprevalence study in patients with requests for laboratory diagnosis of vector-borne viruses. A total of 538 patients with and without neurological signs from 2004 to 2008 were studied by in-house indirect immunofluorescence assay and commercial enzyme-linked immunosorbent assays. A prevalence of 4·2% for IgG antibodies was found in the group of patients with neurological signs. Five (3%) of these had recent infections. In the group with no neurological signs, the IgG prevalence was 1·3%. Two samples, belonging to two patients, were also confirmed with plaque reduction neutralization tests with the TOSV ISS. Phl.3 Italian strain. This work showed that TOSV is present and causing disease from north to south in Portugal. The probable circulation of different phlebovirus serotypes in Portugal emphasizes the need for further studies

Proteomics Profiling of Heterozygous and Homozygous Patients with ABCA1 Gene Mutation: A Tangier Disease Molecular Map

Tangier Disease (TD) is a rare inherited disorder with approximately 100 worldwide identified cases. Alpha-lipoprotein deficiency is the main characteristic of this disease, associated with a virtual absence of High Density Lipoproteins (HDL) in blood. Additional symptoms are mild hypertriglyceridemia, neuropathy and enlarged, orange-colored tonsils. Genetically TD is caused by mutations in the ABCA1 gene, which prevent the release of cholesterol and phospholipids from cells, leading to the accumulation of lipids within cells and body tissues. In this work a TD patient and his parental heterozygous were examined from a proteomics point of view. Plasma as well as proteome and secretome of circulating monocytes were analyzed. Plasma proteins underlined in TD the imbalance of lipid trafficking and metabolism, associated with the stimulation of pro-inflammatory pathways. Proteome and secretome of monocytes highlighted an extensive down regulation of mitochondrial enzymes and vesicular trafficking agents along with a substantial cytoskeletal rearrangement, suggesting a reduced activation state of monocytes from TD homozygous patient. This work is the first proteomics profiling of heterozygous and homozygous TD phenotypes and it suggests a TD case as a model to understand general mechanisms of lipid transport and metabolism and their linkage to inflammatory processes