The DCU laser ion source

Laser ion sources are used to generate and deliver highly charged ions of various masses and energies. We present details on the design and basic parameters of the DCU laser ion source (LIS). The theoretical aspects of a high voltage (HV) linear LIS are presented and the main issues surrounding laser-plasma formation, ion extraction and modeling of beam transport in relation to the operation of a LIS are detailed. A range of laser power densities (I ∼ 108–1011 W cm−2) and fluences (F = 0.1–3.9 kJ cm−2) from a Q-switched ruby laser (full-width half-maximum pulse duration ∼ 35 ns, λ = 694 nm) were used to generate a copper plasma. In “basic operating mode,” laser generated plasma ions are electrostatically accelerated using a dc HV bias (5–18 kV). A traditional einzel electrostatic lens system is utilized to transport and collimate the extracted ion beam for detection via a Faraday cup. Peak currents of up to I ∼ 600 μA for Cu+ to Cu3+ ions were recorded. The maximum collected charge reached 94 pC (Cu2+). Hydrodynamic simulations and ion probe diagnostics were used to study the plasma plume within the extraction gap. The system measured performance and electrodynamic simulations indicated that the use of a short field-free (L = 48 mm) region results in rapid expansion of the injected ion beam in the drift tube. This severely limits the efficiency of the electrostatic lens system and consequently the sources performance. Simulations of ion beam dynamics in a “continuous einzel array” were performed and experimentally verified to counter the strong space-charge force present in the ion beam which results from plasma extraction close to the target surface. Ion beam acceleration and injection thus occur at “high pressure.” In “enhanced operating mode,” peak currents of 3.26 mA (Cu2+) were recorded. The collected currents of more highly charged ions (Cu4+–Cu6+) increased considerably in this mode of operation

The Bacterial Photosynthetic Reaction Center as a Model for Membrane Proteins

Membrane proteins participate in many fundamental cellular processes. Until recently, an understanding of the function and properties of membrane proteins was hampered by an absence of structural information at the atomic level. A landmark achievement toward understanding the structure of membrane proteins was the crystallization (1) and structure determination (2-5) the photosynthetic reaction center (RC) from the purple bacteria Rhodopseudomonas viridis, followed by that of the RC from Rhodobacter sphaeroides (6-17). The RC is an integral membrane protein-pigment complex, which carries out the initial steps of photosynthesis (reviewed in 18). RCs from the purple bacteria Rps. viridis and Rb. sphaeroides are composed of three membrane-associated protein subunits (designated L, M, and H), and the following cofactors: four bacteriochlorophylls (Bchl or B), two bacteriopheophytins (Bphe or [phi]), two quinones, and a nonheme iron. The cofactors are organized into two symmetrical branches that are approximately related by a twofold rotation axis (2, 8). A central feature of the structural organization of the RC is the presence of 11 hydrophobic [alpha]-helixes, approximately 20-30 residues long, which are believed to represent the membrane-spanning portion of the RC (3, 9). Five membrane-spanning helixes are present in both the L and M subunits, while a single helix is in the H subunit. The folding of the L and M subunits is similar, consistent with significant sequence similarity between the two chains (19-25). The L and M subunits are approximately related by the same twofold rotation axis that relates the two cofactor branches. RCs are the first membrane proteins to be described at atomic resolution; consequently they provide an important model for discussing the folding of membrane proteins. The structure demonstrates that [alpha]-helical structures may be adopted by integral membrane proteins, and provides confirmation of the utility of hydropathy plots in identifying nonpolar membrane-spanning regions from sequence data. An important distinction between the folding environments of water-soluble proteins and membrane proteins is the large difference in water concentration surrounding the proteins. As a result, hydrophobic interactions (26) play very different roles in stabilizing the tertiary structures of these two classes of proteins; this has important structural consequences. There is a striking difference in surface polarity of membrane and water-soluble proteins. However, the characteristic atomic packing and surface area appear quite similar. A computational method is described for defining the position of the RC in the membrane (10). After localization of the RC structure in the membrane, surface residues in contact with the lipid bilayer were identified. As has been found for soluble globular proteins, surface residues are less well conserved in homologous membrane proteins than the buried, interior residues. Methods based on the variability of residues between homologous proteins are described (13); they are useful (a) in defining surface helical regions of membrane and water-soluble proteins and (b) in assigning the side of these helixes that are exposed to the solvent. A unifying view of protein structure suggests that water-soluble proteins may be considered as modified membrane proteins with covalently attached polar groups that solubilize the proteins in aqueous solution

An Isomorphous Replacement Method for Phasing Twinned Structures

A linear least-squares formulation of the method of isomorphous replacement is presented. With data from untwinned crystals, this approach is shown to be equivalent to the phasing representation developed by Hendrickson & Lattman [Acta Cryst. (1970). B26, 136-143]. A general method for calculating the most probable phase is described and applied to the higher- dimensional problem of phase determination for twinned structures. A method for calculating the best phase with intensity data from twinned crystals is also presented. The dependences of these phasing procedures on the number of derivatives and accuracy of the data sets are evaluated in test calculations

Preparation of Halogenated Derivatives of Thiazolo[5,4-d]thiazole via Direct Electrophilic Aromatic Substitution

Chlorination and bromination reactions of thiazolo[5,4-d]thiazole led to the generation of its mono- and dihalogenated derivatives. These are the first instances of successful direct electrophilic aromatic substitution in the thiazolo[5,4-d]thiazole ring system. X-ray analysis demonstrates that both 2-bromothiazolo[5,4-d]-thiazole and 2,5-dibromothiazolo[5,4-d]thiazole are planar structures, with strongly manifested π-stacking in the solid state. Theoretical analysis of the pyridine-catalyzed halogenation (MP2/6-31+G(d) and B3LYP/6-31+G(d)calculations) reveals that introduction of one halogen actually leads to a slightly enhanced reactivity towards further halogenation. Several halogenation mechanisms have been investigated: 1) The direct C-halogenation with N-halopyridine as electrophile; 2) C-halogenation viaintermediate N-halogenation, and 3) C-halogenation following an addition - elimination pathway, with intermediate formation of a cyclic halonium ion. The theoretical studies suggest that the direct C-halogenation is the favored mechanism

How many active regions are necessary to predict the solar dipole moment?

We test recent claims that the polar field at the end of Cycle 23 was weakened by a small number of large, abnormally oriented regions, and investigate what this means for solar cycle prediction. We isolate the contribution of individual regions from magnetograms for Cycles 21, 22 and 23 using a 2D surface flux transport model, and find that although the top ~10% of contributors tend to define sudden large variations in the axial dipole moment, the cumulative contribution of many weaker regions cannot be ignored. In order to recreate the axial dipole moment to a reasonable degree, many more regions are required in Cycle 23 than in Cycles 21 and 22 when ordered by contribution. We suggest that the negative contribution of the most significant regions of Cycle 23 could indeed be a cause of the weak polar field at the following cycle minimum and the low-amplitude Cycle 24. We also examine the relationship between a region's axial dipole moment contribution and its emergence latitude, flux, and initial axial dipole moment. We find that once the initial dipole moment of a given region has been measured, we can predict the long-term dipole moment contribution using emergence latitude alone.Comment: 15 pages, 10 figures, accepted for publication in Ap

Locating regional health policy: Institutions, politics, and practices

Poverty reduction and health became central in the agendas of Southern regional organisations in the last two decades. Yet, little is known about how these organisations address poverty, inclusion and social inequality, and how Southern regional formations are engaging in power constellations, institutions, processes, interests and ideological positions within different spheres of governance. This article reviews academic literatures spanning global social policy, regional studies and diplomacy studies, and the state of knowledge and understanding of the ‘place’ of regional actors in health governance as a global political practice therein. It identifies theoretical and thematic points of connection between disparate literatures and how these can be bridged through research focusing on the social policies of regional organisations and regional integration processes. This framework hence locates the contributions of each of the research articles of this Special Issue of Global Social Policy on the regional dimension of health policy and diplomacy in relation to Southern Africa and South America. It also highlights the ways in which the articles bring new evidence about how social relations of welfare are being (re)made over larger scales and how regional actors may initiate new norms to improve health rights in international arenas engaging in new forms of ‘regional’ diplomacy

Drought Tolerance of Interspecific Hybrids between \u3ci\u3eTrifolium repens\u3c/i\u3e and \u3ci\u3eTrifolium ambiguum\u3c/i\u3e

Hybrids between the stolonifeous white clover (Trifolium repens L., 2n=4x=32) and rhizomatous Caucasian clover (T. ambiguum M.Bieb, 2n=4x=32) have been produced. A backcross 2 (BC2) generation with white clover as the recurrent parent combines the growth habit of both parent species with the objective of increasing the persistency of large leaved T. repens varieties under grazing. T. ambiguum is more drought tolerant than T. repens. The drought tolerance of the hybrids in comparison with the parental species was compared in deep soil bins over a four week drought cycle. Soil moisture content, leaf relative water content (RWC), and leaf water potential were measured on plants subjected to drought and those watered normally and maintained at field capacity. T. ambiguum and the backcross hybrids were able to maintain a higher leaf RWC and leaf water potential than T. repens at comparable levels of soil moisture. The dry matter production of the hybrids and parental species was also compared in field plots sown with a perennial ryegrass companion. In the first harvest year, under a cutting regime, the yield of T. repens was highest and T. ambiguum lowest with the hybrids intermediate between the parents, with the BC2 approaching the yield of T. repens. These hybrids will be evaluated over further years and under grazing. The implications of these results for T. repens germplasm improvement programmes are discussed