Drie nieuwe Ponto-Kaspische inwijkelingen dringen door tot in kanalen in de provincie Antwerpen: De zoetwaterpolychaet <i>Hypania invalida</i> (Grube, 1860) en, voor het eerst in België, de platworm <i>Dendrocoelum romanodanubiale</i> (Codreanu, 1949) en de Donaupissebed <i>Jaera istri</i> Veuille, 1979

Since 2000 three Ponto-Caspian invaders have been found in canals in the province of Antwerp (N.E.-Belgium): the freshwater polychaete Hypania invalida (Grube, 1860), the triclad Dendrocoelum romanodanubiale (Codreanu, 1949) and the freshwater isopod Jaera istri Veuille, 1979. Staff members of the 'Vlaamse Milieumaatschappij (Flemish Environmental Agency) collected these species on artificial substrates (nets filled with broken bricks). H. invalida had been discovered before in Belgium in the river Meuse in 2000 (Vanden Bossche et.al., 2001). D. romanodanubiale and J. istri were never recorded before in Belgium. Before its discovery in the Belgian Meuse in August-September 2000, H.invalida had already been found in May 2000 in the Albert Canal at Genk in the province of Limbourg. Between 2001 and 2003, the polychaete was sampled at several stations of the Albert Canal and its adjacent canals in the provinces of Antwerp and Limbourg. Here, it often is accompanied by D. romanodanubiale and J. istri. In 2003 the polychaet had also been encountered in the Sea canal Brussels - Scheldt in the province of Antwerp. The discontinuous distribution indicates that navigation plays an important role in the dispersal of the species. Other Ponto-Caspian species such as Dikerogammarus villosus (Sovinskij, 1894) are either already present in the canals or soon to be expected there. Some of these species will probably also invade the Scheldt basin

Identification and in vivo characterization of a brain-penetrating nanobody

BACKGROUND: Preclinical models to determine blood to brain transport ability of therapeutics are often ambiguous. In this study a method is developed that relies on CNS target-engagement and is able to rank brain-penetrating capacities. This method led to the discovery of an anti-transferrin receptor nanobody that is able to deliver a biologically active peptide to the brain via receptor-mediated transcytosis. METHODS: Various nanobodies against the mouse transferrin receptor were fused to neurotensin and injected peripherally in mice. Neurotensin is a neuropeptide that causes hypothermia when present in the brain but is unable to reach the brain from the periphery. Continuous body temperature measurements were used as a readout for brain penetration of nanobody-neurotensin fusions after its peripheral administration. Full temperature curves were analyzed using two-way ANOVA with Dunnett multiple comparisons tests. RESULTS: One anti-transferrin receptor nanobody coupled to neurotensin elicited a drop in body temperature following intravenous injection. Epitope binning indicated that this nanobody bound a distinct transferrin receptor epitope compared to the non-crossing nanobodies. This brain-penetrating nanobody was used to characterize the in vivo hypothermia model. The hypothermic effect caused by neurotensin is dose-dependent and could be used to directly compare peripheral administration routes and various nanobodies in terms of brain exposure. CONCLUSION: This method led to the discovery of an anti-transferrin receptor nanobody that can reach the brain via receptor-mediated transcytosis after peripheral administration. This method could be used to assess novel proteins for brain-penetrating capabilities using a target-engaging readout

Direct magneto-optical compression of an effusive atomic beam for high-resolution focused ion beam application

An atomic rubidium beam formed in a 70 mm long two-dimensional magneto-optical trap (2D MOT), directly loaded from a collimated Knudsen source, is analyzed using laser-induced fluorescence. The longitudinal velocity distribution, the transverse temperature and the flux of the atomic beam are reported. The equivalent transverse reduced brightness of an ion beam with similar properties as the atomic beam is calculated because the beam is developed to be photoionized and applied in a focused ion beam. In a single two-dimensional magneto-optical trapping step an equivalent transverse reduced brightness of $(1.0\substack{+0.8-0.4})$ $\times 10^6$ A/(m$^2$ sr eV) was achieved with a beam flux equivalent to $(0.6\substack{+0.3-0.2})$ nA. The temperature of the beam is further reduced with an optical molasses after the 2D MOT. This increased the equivalent brightness to $(6\substack{+5-2})$$\times 10^6$ A/(m$^2$ sr eV). For currents below 10 pA, for which disorder-induced heating can be suppressed, this number is also a good estimate of the ion beam brightness that can be expected. Such an ion beam brightness would be a six times improvement over the liquid metal ion source and could improve the resolution in focused ion beam nanofabrication.Comment: 10 pages, 8 figures, 1 tabl

Psychometric properties of an interviewer-administered version of the Kessler Psychological Distress scale (K10) among Dutch, Moroccan and Turkish respondents

Sons, Lt

Is the pull-out force of the Meniscus Arrow in bone affected by the inward curling of the barbs during biodegradation? An in vitro study

Background: Inward curling of the barbs of Meniscus Arrows during degradation was observed in a previous study, in which swelling, distention, and water uptake by Meniscus Arrows was evaluated. This change of configuration could have consequences with respect to anchorage capacity in bone. Material/Methods: Eight non-degraded Meniscus Arrows in the original configuration were pulled out of thawed, fresh-frozen human femoral condyle, and pull-out force was measured and compared with that of 6 Meniscus Arrows after 31 days of degradation under controlled conditions. Results: No significant difference was found between the 2 groups with respect to the required pull-out force (t test), the distribution of the data, or the interaction between degradation and location, as evaluated by mann-Whitney test, and no significant difference was found between the 2 groups with respect to the degradation state or position in the coudyles, as evaluated by 2-way analysis of variance. Conclusions: Our results indicate that the decrease in barb-barb diameter during the first month of degradation of the Meniscus Arrows has no significant effect on the tensile pull-out force required for removal from human femur condyle. Further research should be undertaken to examine whether the same is true for other biodegradable devices with barbs

Будівельна лихоманка на Київських схилах

Forty marine-terminating glaciers have been surveyed daily since 2000 using cloud-free MODIS visible imagery (Box and Decker 2011; http://bprc. osu.edu/MODIS/). The net area change of the 40 glaciers during the period of observation has been -1775 km2, with the 18 northernmost (>72°N) glaciers alone contributing to half of the net area change. In 2012, the northernmost glaciers lost a collective area of 255 km2, or 86% of the total net area change of the 40 glaciers surveyed. The six glaciers with the largest net area loss in 2012 were Petermann (-141 km2), 79 glacier (-27 km2), Zachariae (-26 km2), Steenstrup (-19 km2), Steensby (-16 km2, the greatest retreat since observations began), and Jakobshavn (-13 km2). While the total area change was negative in 2012, the area of four of the forty glaciers did increase relative to the end of the 2011 melt season. The anomalous advance of these four glaciers is not easily explained, as the mechanisms controlling the behavior of individual glaciers are uncertain due to their often unique geographic!settings