Pea heat stress-responsive transcriptome analysis and heat tolerance improvement through marker-assisted backcrossing

Non-Peer Reviewe

Evaluation of synchrotron based x-ray fluorescence spectroscopy for quantification of minerals in pea seeds

Non-Peer Reviewe

Pea heat stress-responsive transcriptome analysis

Non-Peer Reviewe

Effect of iron, phytic acid, and carotenoid concentration on iron bioavailability in pea

Non-Peer Reviewe

Evaluation of iron bioavailability in Pea (Pisum sativum L.) lines contrasting in nutritional traits

Non-Peer Reviewe

Mapping of QTL associated with seed phytic acid concentration in pea recombinant inbred lines

Non-Peer Reviewe

Implementation of marker-assisted selection for lodging resistance in pea breeding

Non-Peer ReviewedLodging resistance is a key criterion in pea breeding programs. In traditional breeding, a large number of lines are discarded in F3 or later generations, because of susceptibility to lodging and much labor and other costs are wasted. Implementation of selection using molecular markers for lodging resistance at earlier generations will significantly enhance the efficiency of the pea breeding process. Ten F2 populations consisting of a total of 869 plants were grown in the field in Saskatoon in the summer of 2002. These 10 populations were derived from crosses between the lodging susceptible variety Carrera, crossed with ten other lodging resistant lines. DNA samples were extracted from each individual plant. Each plant was scored for the presence of coupling phase linked SCAR markers A001 and A002, as well as a repulsion phase linked SCAR marker A004. The results showed that the frequency of marker presence is similar to the estimated frequency, obeying the rule of independent single gene segregation. Chi-Square analysis showed that the combination of A001 and A004 markers also followed a two gene model (P>0.05) in 8 populations. Due to linkage between A001 and A002 markers, population No. 3 and No. 4 did not follow the two or three independent gene segregation model. F3 populations will be planted in the field in 2003 to evaluate the effectiveness of Marker Assist Selection for lodging resistance in pea

Leaf pigments and wax as traits of heat tolerance in field pea

Non-Peer Reviewe

Soybean yield and release of nutrients from soybean residue in comparison to pea and lentil in Saskatchewan soils

Non-Peer Reviewe

Mapping QTL associated with yield and yield components and Ascochyta blight in chickpea

Non-Peer ReviewedChickpea (Cicer arietinum L.) is the third important legume crop in production among the world pulse crops. A better understanding of the genetic basis of yield and disease traits and their association with flowering time could contribute to their use in the breeding strategies of the crop in the Canadian Prairies. The objective of this study was to evaluate a set of Recombinant Inbred Lines (RILs) of chickpea for yield and disease traits and to locate Quantitative Trait Loci (QTL) associated with these traits. This study used a RIL population derived from across between ICCV 96029 (a desi market class, an extra early maturing, highly susceptible to ascochyta blight and CDC Frontier (a kabuli market class, late maturing, moderately resistant to ascochyta blight. A population consisting of 92 RILs together with the 2 parents were grown in a field at Elrose, Saskatchewan in 2011 in a micro plot with 2 replications. Measurements on agronomic traits were made on an individual plant basis and the means of five plants were used for analysis. Plants were air-dried at 35°C for 48 h before measuring the above-ground biomass. Traits measured were grain yield (in gm /plant), above ground biomass (in g/ plant), number of grains /plant, number of pods/ plant, and 1000 seed weight. Harvest index (HI) = grain weight/total above ground dry weight. The result indicated that, there was significant difference in plant height (in cm), number of seeds /plant, number of seeds/pod, 1000 seeds weight (in gm/plant) and Harvest Index (%). The same lines were evaluated in the greenhouse for Ascochyta blight reaction and in the growth chambers for their flowering responses to different photoperiod. Mapping of QTL will be performed on the line mean data for single years of the field observation and for different photoperiod treatments in the growth chamber