Shunmugam, A.

Warkentin, T.

English

University of Saskatchewan Research Archive

www.usask.ca  Mapping of QTL associated with seed phytic acid concentration in pea recombinant inbred lines  Arun Shunmugam Crop Development Centre University of Saskatchewan  Soils and Crops  11 March 2014  www.usask.ca Outline • Background   • Phytate in environment  • Low phytate pea lines  • Mapping phytic acid QTL  • Conclusion www.usask.ca Background  • Phytic acid  - Storage form of P - Mixed cationic salt  • Binds K+, Mg++, Ca++, Mn++, Zn++, Fe+++     (Ref: http://www.seedbiology.de) Myo-inositol hexakisphosphoric acid Phytate-Metal Complex www.usask.ca   (Modified from Brinch-Pedersen et al., 2002)    Phytate in environment www.usask.ca • Consequences  - Micro nutrient deficiency  - Environmental phosphorus pollution • Strategies to reduce Phytate  - Fermentation  - Soaking  - Processing   Plant Breeding – Low Phytate Crops  www.usask.ca Low Phytate Pea Lines • Two low phytate (lpa)pea lines  - 1-150-81 and 1-2347-144  (Warkentin. T.D., et.al, 2012) - Chemical mutagenesis of CDC Bronco   (Raboy.V., Plant Science 2009) www.usask.ca What do we know about these lpa pea lines?  • Reduced Phytate-P concentration (Warkentin. T.D., et al, 2012) - approx 60% reduction •  High in Inorganic Phosphorus •  Agronomic performance   -    similar to CDC Bronco   -     slower flowering time and maturity    -     lower seed weight and yield  www.usask.ca • Hypothesis     QTL associated with seed phytic acid concentration can be  identified from the recombinant inbred lines developed  • Objective    Mapping of QTL associated with seed phytic acid concentration in pea recombinant inbred lines   Mapping of QTL for Phytate-P concentration www.usask.ca   Materials and Methods •   PR-15   - Recombinant Inbred lines              -  Sutherland 2011               -  Sutherland & Rosthern 2012,                   2013  •   Phenotyping  -  Phytate –P estimation   •   Genotyping  -  GoldenGate assay  -  1536 SNP markers  • Statistics - SAS 9.3 PROC MIXED  www.usask.ca   0510152025303540No.of lines Phytate P concentration (µg/gm) 1-2347-144 CDC Meadow 0510152025303540No.of  lines Phytate P concentration (µg/gm) 1-2347-144 CDC Meadow Results – Phytate-P concentration Distribution of Phytate P in PR-15 lines at Sutherland 2011 Distribution of Phytate P in PR-15 lines at Sutherland & Rosthern 2012 www.usask.ca   0510152025303540No.of lines Phytate P concentration (µg/gm) 1-2347-144 CDC Meadow Distribution of Phytate P in PR-15 lines at Sutherland & Rosthern 2013 Results – Phytate-P concentration ANOVA for phytate-P concentration in 2012 & 2013 Effect Num DF Den DF F Value Pr > F Variety 164 344 2.45 <.0001 Year 1 2 2.57 0.2498 Year*Variety 164 344 1.45 0.0025 www.usask.ca Group 7 Group 2-A Group 1-A Group 1-B Group 1-C Group 3-A Group 4-A Group 4-B Group 5-B Group 6-A Group 6-C Group 6-D Group 6-E Group 2-B Group 5-A Group 3-B Group 6-B PR-15 Linkage Map with SNP markers www.usask.ca Results – QTL for Phytate P PsC13258p940.0PsC6041p3030.8PsC4586p20813.8PsC12754p28314.1PsC1538p63414.4PsC17841p14914.5PsC2509p330 PsC12474p40414.9PsC18987p64 PsC7666p40616.7PsC33p15618.2PsC9429p143 PsC15521p171PsC9867p264 PsC9429p300PsC11390p169 PsC4035p224PsC14876p24519.1PsC466p12722.4PsC5316p23423.9PsC25202p29924.9PsC13167p28127.5PsC13505p7630.4PsC6084p29630.8PsC4486p127 PsC14118p30345.2PsC15410p234 PsC7692p316PsC11912p15247.1PsC65p8152.5PsC6129p16054.0PsC16039p13554.3PsC20188p35154.5PsC19062p7354.6PsC8547p15755.7PsC20442p333 PsC8015p30756.1PsC7848p9556.4PsC9736p8456.7PsC18968p13557.1GenoPlot display in GenomeStudio showing PR-15 lines with 2 distinct clusters for PsC14876p245 SNP marker PsC14876p245 associated with phytate-P in PR-15  www.usask.ca   QTL associated with Phytate-P in PR-15 lines across 5 site years Results – QTL for Phytate P Year Locus Linkage Group LOD Phenotypic variation (%) Sutherland 2011 PsC14876p245 5-A 21.96 47.1 Sutherland 2012 PsC14876p245 5-A 19.1 42.5 Rosthern 2012 PsC14876p245 5-A 20.68 45.1 Sutherland 2013 PsC14876p245 5-A 20.11 44.2 Rosthern2013 PsC14876p245 5-A 20.46 44.7 www.usask.ca Conclusion • Phytate P QTL  - consistent in all 5 site years  - explains high phenotypic variation  • Validation of QTL  - in PR-15 and other lines  What are the possible outcomes of this project? • SNP marker -Marker assisted selection (MAS) • Lpa pea varieties with improved mineral nutrition • Reduced environmental pollution  We don’t eat nutrients….  We eat food that gives us nutrients…. www.usask.ca  Dr. Tom Warkentin, CDC,      University of Saskatchewan   Dr. Gene Arganosa Crop Science Field Lab Crew Technicians at Crop Development Centre, University of Saskatchewan  Acknowledgement  

Mapping of QTL associated with seed phytic acid concentration in pea recombinant inbred lines

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https://harvest.usask.ca/bitstream/10388/8919/1/A.%20Shunmugam%20and%20T.%20Warkentin%2c%202014.pdf

Mapping of QTL associated with seed phytic acid concentration in pea recombinant inbred lines

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