Productivity of a Triticale and Crimson Clover Winter Cover Crop for Dairies

The potential for a winter cover crop to align with agronomic objectives and to support milk production was evaluated at the Kansas State University Dairy Teaching and Research Center, Manhattan, KS. August planting of a triticale and crimson clover blend following corn silage harvest resulted in production of more than 3.5 tons of dry matter prior to subsequent corn planting. After ensiling, the impact of triticale/crimson clover silage (TCS) on milk production was evaluated in 48 mid- to late-lactation Holstein cows. Cows were blocked by parity (1 and 2+) and milk production, then randomly assigned within block to treatment sequence and pen. The crossover design consisted of two 21-day periods, with 17 days of diet adaptation and 4 days of sampling. Treatments were a diet which included TCS at 15% of diet dry matter (DM) and a control ration in which TCS was primarily replaced by alfalfa and grass hays. The TCS diet included additional bypass soybean meal in an attempt to balance metabolizable protein supply across diets. Samples of rations, feed refusals, and milk were obtained daily, and milk yield was recorded. The TCS diet decreased dry matter intake (48.4 vs. 55.9 ± 3.4 lb/d; P = 0.02), but did not alter milk yield (P = 0.97); therefore, feed efficiency was greater for the TCS diet (P = 0.04). Milk fat concentration tended to increase on the TCS diet (P \u3c 0.10) whereas milk lactose yield tended to be lesser for TCS (P = 0.09), but other milk components analyzed (milk protein, MUN, SCC) did not differ between diets (P \u3e 0.15). Utilization of TCS also impacted the dairy’s nutrient management plan, as the winter forage harvest removed 40 and 340 lb/a of phosphorus and potassium, respectively. Overall, the blend of triticale and crimson clover as a winter cover crop produced good quality silage that maintained high milk production while also removing key nutrients from the soil to benefit nutrient management planning

Direct dark matter search by annual modulation in XMASS-I

A search for dark matter was conducted by looking for an annual modulation signal due to the Earth's rotation around the Sun using XMASS, a single phase liquid xenon detector. The data used for this analysis was 359.2 live days times 832 kg of exposure accumulated between November 2013 and March 2015. When we assume Weakly Interacting Massive Particle (WIMP) dark matter elastically scattering on the target nuclei, the exclusion upper limit of the WIMP-nucleon cross section 4.3$\times$10$^{-41}$cm$^2$ at 8 GeV/c$^2$ was obtained and we exclude almost all the DAMA/LIBRA allowed region in the 6 to 16 GeV/c$^2$ range at $\sim$10$^{-40}$cm$^2$. The result of a simple modulation analysis, without assuming any specific dark matter model but including electron/$\gamma$ events, showed a slight negative amplitude. The $p$-values obtained with two independent analyses are 0.014 and 0.068 for null hypothesis, respectively. we obtained 90\% C.L. upper bounds that can be used to test various models. This is the first extensive annual modulation search probing this region with an exposure comparable to DAMA/LIBRA.Comment: 5 pages, 4 figure

Search for two-neutrino double electron capture on 124^{124}Xe with the XMASS-I detector

Double electron capture is a rare nuclear decay process in which two orbital electrons are captured simultaneously in the same nucleus. Measurement of its two-neutrino mode would provide a new reference for the calculation of nuclear matrix elements whereas observation of its neutrinoless mode would demonstrate lepton number violation. A search for two-neutrino double electron capture on $^{124}$Xe is performed using 165.9 days of data collected with the XMASS-I liquid xenon detector. No significant excess above background was observed and we set a lower limit on the half-life as $4.7 \times 10^{21}$ years at 90% confidence level. The obtained limit has ruled out parts of some theoretical expectations. We obtain a lower limit on the $^{126}$Xe two-neutrino double electron capture half-life of $4.3 \times 10^{21}$ years at 90% confidence level as well.Comment: 6 pages, 3 figures, accepted for publication in Physics Letters

Synthesis of Glass Nanofibers Using Femtosecond Laser Radiation Under Ambient Condition

We report the unique growth of nanofibers in silica and borosilicate glass using femtosecond laser radiation at 8 MHz repetition rate and a pulse width of 214 fs in air at atmospheric pressure. The nanofibers are grown perpendicular to the substrate surface from the molten material in laser-drilled microvias where they intertwine and bundle up above the surface. The fibers are few tens of nanometers in thickness and up to several millimeters in length. Further, it is found that at some places nanoparticles are attached to the fiber surface along its length. Nanofiber growth is explained by the process of nanojets formed in the molten liquid due to pressure gradient induced from the laser pulses and subsequently drawn into fibers by the intense plasma pressure. The attachment of nanoparticles is due to the condensation of vapor in the plasma

Influence of polymer-coated slow-release urea on total tract apparent digestibility, ruminal fermentation and performance of Nellore steers

Objective Two experiments were performed to evaluate the effects of coated slow-release urea on nutrient digestion, ruminal fermentation, nitrogen utilization, blood glucose and urea concentration (Exp 1), and average daily gain (ADG; Exp 2) of steers. Methods Exp 1: Eight ruminally fistulated steers [503±28.5 kg body weight (BW)] were distributed into a d 4×4 Latin square design and assigned to treatments: control (CON), feed grade urea (U2), polymer-coated slow-release urea A (SRA2), and polymer-coated slow-release urea B (SRB2). Dietary urea sources were set at 20 g/kg DM. Exp 2: 84 steers (350.5±26.5 kg initial BW) were distributed to treatments: CON, FGU at 10 or 20 g/kg diet DM (U1 and U2, respectively), coated SRA2 at 10 or 20 g/kg diet DM (SRA1 and SRA2, respectively), and coated SRB at 10 or 20 g/kg diet DM (SRB1 and SRB2, respectively). Results Exp 1: Urea treatments (U2+SRA2+SRB2) decreased (7.4%, p = 0.03) the DM intake and increased (11.4%, p<0.01) crude protein digestibility. Coated slow-release urea (SRA2+SRB2) showed similar nutrient digestibility compwared to feed grade urea (FGU). However, steers fed SRB2 had higher (p = 0.02) DM digestibility compared to those fed SRA2. Urea sources did not affect ruminal fermentation when compared to CON. Although, coated slow-release urea showed lower (p = 0.01) concentration of NH3-N (−10.4%) and acetate to propionate ratio than U2. Coated slow-release urea showed lower (p = 0.02) urinary N and blood urea concentration compared to FGU. Exp 2: Urea sources decreased (p = 0.01) the ADG in relation to CON. Animals fed urea sources at 10 g/kg DM showed higher (12.33%, p = 0.01) ADG compared to those fed urea at 20 g/kg DM. Conclusion Feeding urea decreased the nutrient intake without largely affected the nutrient digestibility. In addition, polymer-coated slow-release urea sources decreased ruminal ammonia concentration and increased ruminal propionate production. Urea at 20 g/kg DM, regardless of source, decreased ADG compared both to CON and diets with urea at 10 g/kg DM

Long-term culture of cholangiocytes from liver fibro-granulomatous lesions

BACKGROUND: Extensive bile duct proliferation is a key feature of the tissue reaction to clinical and experimental forms of liver injury. Experimental infection of mice by Schistosoma mansoni is a well-studied model of liver fibrosis with bile duct hyperplasia. However, the regulatory mechanisms of bile duct changes are not well understood. In this study we report the reproducible isolation of long-term cultures of cholangiocytes from mice livers with schistosomal fibrosis. METHODS: We have isolated a cholangiocyte cell line from Schistosoma-induced liver granulomas using a combination of methods including selective adhesion and isopyknic centrifugation in Percoll. RESULTS: The cell line was characterized by morphological criteria in optical and transmission electron microscopy, ability to form well differentiated ductular structures in collagen gels and by a positive staining for cytokeratin 18 and cytokeratin 19. To our knowledge, this is the first murine cholangiocyte cell line isolated from schistosomal fibrosis reported in the literature. CONCLUSION: After 9 months and 16 passages this diploid cell line maintained differentiated characteristics and a high proliferative capacity. We believe the method described here may be a valuable tool to study bile duct changes during hepatic injury