LOW-PROTEIN PASTA FOR CHILDREN PATIENTS WITH PHENYLKETONURIA

There are provided data on the work carried out at All-Russian Research Institute for Starch Products to create enriched low-protein pasta based on starch for nutrition the children sick with a phenilketonuria — a hereditary disease (group of fermentopatiya), the bound to violation of amino acids metabolism, mainly phenyl alanine. Optimization of children nutrition is the socially important direction of domestic policy of the Russian Federation since their health directly depends on the good balanced nutrition, especially it is important for the children having diseases of genetic character.To expand the range of the enriched low-protein food the functional ingredients are picked up, recipes and technology of receiving three types of pasta are developed such as: noodles, vermicelli, «spider line», their nutrition and power values are defined. It is shown that in the received products protein content doesn’t exceed permissible value (<1.0%), fat from 3.3 to 3.6%, ashes — to 0.14%, carbohydrates no more than 88.0% that conforms to requirements imposed to reduced-protein products of baby food.There are provided data on the work carried out at All-Russian Research Institute for Starch Products to create enriched low-protein pasta based on starch for nutrition the children sick with a phenilketonuria — a hereditary disease (group of fermentopatiya), the bound to violation of amino acids metabolism, mainly phenyl alanine. Optimization of children nutrition is the socially important direction of domestic policy of the Russian Federation since their health directly depends on the good balanced nutrition, especially it is important for the children having diseases of genetic character.To expand the range of the enriched low-protein food the functional ingredients are picked up, recipes and technology of receiving three types of pasta are developed such as: noodles, vermicelli, «spider line», their nutrition and power values are defined. It is shown that in the received products protein content doesn’t exceed permissible value (<1.0%), fat from 3.3 to 3.6%, ashes — to 0.14%, carbohydrates no more than 88.0% that conforms to requirements imposed to reduced-protein products of baby food

Methodological aspects of the use of dry components of chicken eggs for feeding children with phenylketonuria

Currently, one of the most important tasks facing science and production is the creation of functional product technologies for use in different diets of the population in order to preserve and improve health, as well as reduce the risks and consequences of various diseases, including hereditary ones, such as phenylketonuria (PKU). The All-Russian Research Institute of Starch Products develops technologies for the production of low-protein starch-based products/semi-products enriched with functional ingredients and intended for therapeutic nutrition of patients with PKU. As part of the pilot production, the production of these products is organized. Purpose of work:to justify the possibility of using dry components of chicken eggs (melange, protein, yolk) to enrich low-protein starch products (noodles, vermichel, «spider») intended for feeding children over 3 years old with phenylketonuria;evaluate organoleptic properties and efficiency of low-protein starch products enriched in hypophenylalanine diet of patients with phenylketonuria older than 3 years

Medico-social rationale for adaptation of regional drug supply systems to personalized pharmaceutical care for persons of different ages

In this study, we identified indicators of the state of personalized pharmaceutical care for target population groups at the regional level, in a model region, with the aim to develop a differentiated approach to such care. The rationale for the choice of Moscow Region as a model region is based on its specific socioeconomic indicators and a comparison of these with respective average Russian values. A need was shown to modernize patient-oriented pharmaceutical care, especially for certain population groups, such as children, women of working age (mothers), and the elderl

Tyrosine hydroxylase of the brain and it’s regulation by glucocorticoids

Early life stress events can produce long-lasting changes in neurochemistry and behaviors related to monoamine systems, with increased risks of cardiovascular, metabolic, neuroendocrine, psychiatric disorders, generalized anxiety and depression in adulthood. Tyrosine hydroxylase (TH), the key enzyme for catecholamine synthesis, also plays an important role in the activity of the noradrenergic system and may be a target for glucocorticoids during the perinatal programming of physiological functions and behavior. Administration of hydrocortisone or dexamethasone to female rats on day 20 of pregnancy and to 3-day-old neonatal pups significantly increased TH mRNA levels (real-time PCR) and enzyme activity as well as protein levels determined by ICH in the locus coeruleus. Moreover, our treatment led to increase in TH mRNA levels in 25- and 70-day-old animals, as well as an increase in enzyme activity in the brainstem and cerebral cortex of adult rats. The long-term changes in TH expression are limited by the perinatal period of development. Administration of hormones on day 8 of life was not accompanied by changes in TH mRNA levels or enzyme activity. Glucocorticoids use several mechanisms to bring about transactivation or transrepression of genes. The main mechanism includes direct binding of the hormone-activated GRs to glucocorticoid responsive elements (GREs) in the promoter region of genes. However, despite optimistic claims made the classical GRE was not found in the TH gene promoter. Protein – protein interactions between hormone-activated GR and other transcription factors, for example, AP-1, provide an additional mechanism for the effects of glucocorticoids on gene expression. An important feature of this mechanism is its dependence on the composition of proteins formed by AP-1. Hormone-activated GRs are able to enhance gene expression when AP-1 consists of the Jun / Jun homodimer, but do not do that when AP-1 appears as the Jun / Fos heterodimer. Furthermore, as has been shown recently, the GRE / AP-1 composite site is the major site of interaction of glucocorticoids with  the TH gene in the pheochromocytoma cell line. Ontogenetic variation in the expression of Fos and Jun family proteins, which affects their ratio, can be one of the reasons for the TH gene regulation by glucocorticoids at near-term fetuses and neonates. However, to date this hypothesis has been supported only by in vitro data, and the existence of this mechanism in in vivo conditions needs to be explored in further studies

Observation of core electron temperature rise in response to an edge cooling in toroidal helical plasmas

The first observation of a significant rise of core electron temperature in response to edge cooling in a helical plasma has been made on the Large Helical Device [O. Motojima et al., Phys. Plasmas 6, 1843 (1999)]. When the phenomenon occurs, the electron heat diffusivity in the core region is reduced abruptly without changing local parameters in the region of interest. Therefore the phenomenon can be regarded as a so-called "nonlocal" electron temperature rise observed so far only in many tokamaks

Tissue-specific effects of benzo[a]pyrene and DDT on microRNA expression profile in female rats

Many xenobiotics in the human environment, such as benzo[a]pyrene (B(a)P) and dichlorodiphenyltrichloroethane (DDT), may act as non-genotoxic carcinogens through epigenetic mechanisms, including changes in microRNA expression profile. In part, such disorders can be mediated by the activation of nuclear receptors, resulting in the activation of protein coding gene expression and microRNAs involved in malignant transformation of cells. Therefore, the aim of this study was to investigate the chain of events “xenobiotic administration – receptor activation – up-regulating microRNA expression – down-regulation target genes expression” as one of the key factors in the chemically-induced carcinogenesis. Using in silico methods, an analysis of the rat genome was carried out to find microRNAs putatively regulated by AhR (aryl hydrocarbon receptor) and CAR (constitutive androstane receptor), activated by BP and DDT, respectively. In particular, miR-3577 and -193b were selected as potentially regulated CAR, miR-207 was selected as a candidate for miR under AhR regulation. The results of the study showed that the treatment of female rats with DDT and B(a)P caused a tissue-specific changes in the expression of microRNAs and host genes in both acute and chronic administration of xenobiotics. To confirm the effects of xenobiotics on the microRNA expression, we also estimated the mRNA level of PTPN6, EIF3F, Cbx7, and Dicer1 genes potentially targeting miR-193b, -207, and -3577. The study has shown a high correlation between the expression of target genes and microRNAs; however these changes depended on the tissue types, the dose and time after xenobiotic treatment

Synthesis of tetrazole containing 1,2,3-thiadiazole derivatives via U-4CR and their anti-TMV activity

A series of novel tetrazole containing 1,2,3-thiadiazole derivatives were designed and synthesized via Ugi reaction. Their structures were confirmed by melting points, IR, 1H NMR, and HRMS (ESI). Preliminary bioassay indicated that most target compounds exhibited very good direct anti-TMV activity at 100 μg/mL, which was equal to or higher than that of ribavirin. Among them, compounds 4b, 4c and 4i also showed equivalent protection effect to ribavirin in vivo at 100 μg/mL. © 2013 Zhi-Jin Fan. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved

Синтез и исследование цис-транс-изомерии 5-(4-метоксибензоил)-6-(4-метоксифенил)-6,7-дигидро-5H-[1,2,3]триазоло[5,1-b][1,3,4]тиадиазина

It was shown that the reaction of 1,2,3-4-methoxybenzaldehyde tiadiazolyl hydrazona and α-bromo-4-methoxyacetophenone in the presence of triethylamine allowes 5-(4-methoxybenzoyl)-6-(4-methoxyphenyl)-6,7-dihydro-5H-[1,2,3]triazolo[5,1-b][1,3,4]thiadiazine as a mixture of cis- and trans-stereoisomers. The influence of nature of the solvent and temperature on the ratio of isomers was studied. An increase of the relative amount of the trans-stereoisomer with increasing polarity of the solvent is disclosed. It is noted that the temperature does not affect the ratio of isomers.Показано, что при взаимодействии 1,2,3-тиадиазолилгидразона 4-метоксибензальдегида с α-бром-4-метоксиацетофеноном в присутствии триэтиламина образуется 5-(4-метоксибензоил)-6-(4-метоксифенил)-6,7-дигидро-5H-[1,2,3]триазоло[5,1-b][1,3,4]тиадиазин в виде смеси цис- и транс- стереоизомеров. Исследовано влияние на соотношение изомеров природы растворителя и температуры. Выявлено увеличение относительного количества транс-стереоизомера с увеличением полярности растворителя

Metrological evaluation of methods for control the safety indicators of leukoreduced platelet concentrates

Objective. The aim of research was to evaluate experimentally the feasibility of using of control methods of safety indicators of leukoreduced platelet concentrates (CPl). Material and methods. The analysis of 14 leukoreduced platelet concentrates samples was performed. Platelet count was estimated in a 2-grid Goryaev’s camera using a «Mikmed-1» light microscope and conductometry with hydrodynamic focusing on an «XT-4000i» hematology analyzer. To determine the residual number of leukocytes, we used the laser flow cytofluorimetry method by «FACS Canto II» analyzer. The pH was measured by the potentiometric method by a pH-millivoltmeter «pH-150M». The feasibility of applying safety performance monitoring techniques was assessed based on the basis of coefficient of variation (CV). Results and discussion. The maximum CV value when calculating the platelet count in the Goryaev’s camera was 18,6 %; conductometry method – 2,8 %. CV when determining the residual number of leukocytes by laser flow cytofluorimetry did not exceed 17,5 %. The CV of the method for determining pH by potentiometric method was 0,4 %. Conclusion. To control the platelet count, a unified method of counting in a 2-grid Goryaev’s camera and conductometry method with hydrodynamic focusing on hematology analyzer are recommended. Laser flow cytofluorimetry should be used to determine the residual number of leukocytes. The maximum CV values were taken as the assigned convergence characteristics: for the unified counting method – not more than 18,6 %, for the conductometry method – not more than 2,8 %, for laser flow cytofluorimetry – not more than 17,5 %, for potentiometry – not more than 0,4 %