44 research outputs found

    Assessment of an in vitro whole cigarette smoke exposure system: The Borgwaldt RM20S 8-syringe smoking machine

    Get PDF
    <p>Abstract</p> <p>Background</p> <p>There have been many recent developments of <it>in vitro </it>cigarette smoke systems closely replicating <it>in vivo </it>exposures. The Borgwaldt RM20S smoking machine (RM20S) enables the serial dilution and delivery of cigarette smoke to exposure chambers for <it>in vitro </it>analyses. In this study we have demonstrated reliability and robustness testing of the RM20S in delivering smoke to <it>in vitro </it>cultures using an in-house designed whole smoke exposure chamber.</p> <p>Results</p> <p>The syringe precision and accuracy of smoke dose generated by the RM20S was assessed using a methane gas standard and resulted in a repeatability error of ≤9%. Differential electrical mobility particle spectrometry (DMS) measured smoke particles generated from reference 3R4F cigarettes at points along the RM20S. 53% ± 5.9% of particles by mass reached the chamber, the remainder deposited in the syringe or connecting tubing and ~16% deposited in the chamber. Spectrofluorometric quantification of particle deposition within chambers indicated a positive correlation between smoke concentration and particle deposition. <it>In vitro </it>air-liquid interface (ALI) cultures (H292 lung epithelial cells), exposed to whole smoke (1:60 dilution (smoke:air, equivalent to ~5 μg/cm<sup>2</sup>)) demonstrated uniform smoke delivery within the chamber.</p> <p>Conclusions</p> <p>These results suggest this smoke exposure system is a reliable and repeatable method of generating and exposing ALI <it>in vitro </it>cultures to cigarette smoke. This system will enable the evaluation of future tobacco products and individual components of cigarette smoke and may be used as an alternative <it>in vitro </it>tool for evaluating other aerosols and gaseous mixtures such as air pollutants, inhaled pharmaceuticals and cosmetics.</p

    Dynamic feedback linearization of the induction motor

    No full text

    Wing Stiffness on Light Flapping Micro Aerial Vehicles

    No full text
    [[abstract]]In an effort to develop micro-aerial-vehicles (MAVs) of 20 cm-span with a successful flight record, this present study aims to investigate the influences of wing configuration and foil stiffness on the aerodynamic forces via wind tunnel testing. These wings with the same wing skeleton structures are made of polyethylene (PE), polyethyleneterephthalate (PET), and polyxylylene (parylene). The foil thickness ranges from 17 to 43 um which corresponds to the variating stiffness of the membrane. The tests showed that the lift coefficients of the stiffened wings have been improved, while the net thrust coefficients have little importance with the wing foil thickness. Inspection on the relations between power consumption and the flexural stiffness of tested flapping wings reveals that the larger lift force and greater power consumption is associated with the 35 and 43 um thick parylene. Therefore, The present study demonstrated that the flight test of a MAV with the best wing foil of 24 um thick PET resulted in the longest endurance time of 8 min. Several innovative aspects of developing this work are also summarized.[[notice]]補正完畢[[journaltype]]國外[[incitationindex]]SCI[[ispeerreviewed]]Y[[booktype]]紙本[[countrycodes]]US
    corecore