Nosocomial Infection Prevention Through Universal Precaution in YOGYAKARTA Muhammadiyah Hospital

Prevention of nosocomial infections in hospitals can be done through the implementation of universal precaution program or action asepsis and antisepsis tapping. These actions undertaken by health workers, both nurses and physicians, universal precaution measures include: hand washing, use of gloves, use aseptic liquid, processing of used equipment and waste disposal. The purpose of this study to determine the prevention of nosocomial infections in hospitals through universal precautions in hospitals Muhammadiyah Yogyakarta area. Design of this research is descriptive qualitative case study approach. Collecting data used techniques focus group discussions. Data analyzed using content analysis. The application of universal precautions in hospitals throughout Yogyakarta PKU show that health workers have attempted to implement universal precautions including hand washing action on the water flow, perform hand hygiene, and always tries to wear personal protective equipment. Nurses knowledge about nosocomial infections has been well and for prevention need to implement universal precautions. The attitude of nurses in implementing universal precautions have been good, to the extent responsible. Most hospitals have had the availability of facilities and support the implementation of universal precaution well, and partly still exist limitations in the availability of facilities and support the implementation of universal precaution

Effect of Perception, Attitude, Patient Safety Policy, Stakeholder Support, and Health Promotion, on Infection Prevention

BACKGROUND: Patients who receive health service, health worker, and visitors in a hospital are at risk for nosocomial infection, ie infection acquired in hospital. The presence of patient safety policy and health promotion are needed for surgical infection prevention effort. This study aimed to analyze the effect of health worker perception, attitude, patient safety policy, stakeholder support, and health promotion, on surgical infection prevention effort. SUBJECT AND METHODS: This was a cross sectional study conducted at PKU Muhammadiyah Hospital, Yogyakarta, Indonesia. A sample of 104 health care workers from this hospital was selected at random for this study. The dependent variable was surgical infection prevention effort. The independent variables were the health worker perception on surgical infection prevention effort, attitude, presence of patient safety policy, stakeholder support, and health promotion. The data were collected using a questionnaire and in-depth interview. The data were analyzed using path analysis model. RESULTS: Health workers perception had positive effect on surgical infection prevention effort, and it was statistically significant (b =0.45; p<0.001). The effects of attitude (b=0.08; p=0.311), presence of patient safety (b=0.06; p=0.413), stakeholder support (b=0.13; p=0.134), and health promotion (b=0.04; p=0.719) on surgical infection prevention effort were nearly non-existent, and statistically non-significant. The model fit indices met the requirement for path analysis model: x2=4.48; p=0.214; GFI=0.98; AGFI=0.88; RMSEA=0.069; NFI=0.98; CFI=0.99. CONCLUSION: Health workers perception affects surgical infection prevention effort. Attitude, presence of patient safety policy, stakeholder support, and health promotion, do not affect surgical infection prevention effort. Keyword: perception, attitude, patient safety, stakeholder support, health promotion, surgical infection prevention effor

KARAKTERISASI GEN PENYANDI PEDIOSIN PAF-11 PADA Pediococcus Acidilactici F-11 [Characterization of the Pediocin PaF-11 Encoding Gene in Pediococcus Acidilactici F-11]

Pediocin PaF-11 is a ribosomally synthesized antimicrobial peptide produced by Pediococcus acidilactici F-11. The objectives of this research is to find out the location and the nucleotide sequence of gene, which is involved in the production of pediocin PaF-11. Results showed that the pediocin PaF-11 from the cured cell of P. acidilactici F-11 loss the activity, suggested that the pediocin PaF-11 gene was carried in the plasmid. Agarose gel electrophoresis of P. acidilactici F-11 plasmid DNA with marker λDNA/HindIII showed that pediocin PaF-11 gene was carried in 12 kb plasmid. Amplification pediocin PaF-11 gene from P. acidilactici F-11 showed that uncured P.acidilactici F-11 culture contain plasmid DNA, indicated by amplification of the papA gene (256 bp). Cured P. acidilactici F-11 culture, plasmid eliminated, indicted by no aplicon DNA detected. This result also suggested that pediocin PaF-11 gene in P. acidilactici F-11 was carried in plasmid. Nucleotide of pediocin PaF-11 encoding gene was sequenced The alignment of that nucleotide sequence showed that pediocin PaF-11 encoding gene have the same sequence with pediocin PA.1 encoding gene in P. acidilactici PAC1.0 and P. acidilactici K10 and pediocin AcH encoding gene in P. acidilactici LB 42-923 and P .parvulus ATO77, and pediocin CP2 in P. acidilactici MTCC 5101