24 research outputs found
CRISPR-assisted detection of RNA-protein interactions in living cells.
Get PDFWe have developed CRISPR-assisted RNA-protein interaction detection method (CARPID), which leverages CRISPR-CasRx-based RNA targeting and proximity labeling to identify binding proteins of specific long non-coding RNAs (lncRNAs) in the native cellular context. We applied CARPID to the nuclear lncRNA XIST, and it captured a list of known interacting proteins and multiple previously uncharacterized binding proteins. We generalized CARPID to explore binders of the lncRNAs DANCR and MALAT1, revealing the method's wide applicability in identifying RNA-binding proteins
Evidence of reproductive isolation among closely related sympatric species of Serrasalmus (Ostariophysii, Characidae) from the Upper Madeira River, Amazon, Bolivia
No full textInfluence of a brisk walking program on isokinetic muscular capacities of knee in sedentary older women
No full textTometes camunani (Characiformes: Serrasalmidae), a new species of phytophagous fish from the Guiana Shield, rio Trombetas basin, Brazil
No full textPRC1 collaborates with SMCHD1 to fold the X-chromosome and spread Xist RNA between chromosome compartments
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Combined RNA-seq and RAT-seq mapping of long noncoding RNAs in pluripotent reprogramming
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