Odour Maps in the Brain of Butterflies with Divergent Host-Plant Preferences

Butterflies are believed to use mainly visual cues when searching for food and oviposition sites despite that their olfactory system is morphologically similar to their nocturnal relatives, the moths. The olfactory ability in butterflies has, however, not been thoroughly investigated. Therefore, we performed the first study of odour representation in the primary olfactory centre, the antennal lobes, of butterflies. Host plant range is highly variable within the butterfly family Nymphalidae, with extreme specialists and wide generalists found even among closely related species. Here we measured odour evoked Ca2+ activity in the antennal lobes of two nymphalid species with diverging host plant preferences, the specialist Aglais urticae and the generalist Polygonia c-album. The butterflies responded with stimulus-specific combinations of activated glomeruli to single plant-related compounds and to extracts of host and non-host plants. In general, responses were similar between the species. However, the specialist A. urticae responded more specifically to its preferred host plant, stinging nettle, than P. c-album. In addition, we found a species-specific difference both in correlation between responses to two common green leaf volatiles and the sensitivity to these compounds. Our results indicate that these butterflies have the ability to detect and to discriminate between different plant-related odorants

Can Persistence be a Matter of Convention?

This paper asks whether persistence can be a matter of convention. It argues that in a rather unexciting de dicto sense persistence is indeed a matter of convention, but it rejects the notion that persistence can be a matter of convention in a more substantial de re sense. However, scenarios can be imagined that appear to involve conventional persistence of the latter kind. Since there are strong reasons for thinking that such conventionality is impossible, it is desirable that our metaphysical-cum-semantic theories of persistence be able to account for such scenarios in terms of conventions of the first kind. Later parts of the article therefore investigate whether three of the currently most influential metaphysical-cum-semantic theories of persistence—the endurance theory, the stage theory, and the perdurance theory—can do this. Fortunately, for them, it turns out that all can, though some philosophers have disputed this. However, when we ask how they account for a typical case of “conventional persistence” some problematic features of the theories—having to do with reference, persistence conditions, how they relate, and the epistemology of persistence—are revealed

An affibody in complex with a target protein: Structure and coupled folding

Combinatorial protein engineering provides powerful means for functional selection of novel binding proteins. One class of engineered binding proteins, denoted affibodies, is based on the three-helix scaffold of the Z domain derived from staphylococcal protein A. The Z(SPA-1) affibody has been selected from a phage-displayed library as a binder to protein A. Z(SPA-1) also binds with micromolar affinity to its own ancestor, the Z domain. We have characterized the Z(SPA-1) affibody in its uncomplexed state and determined the solution structure of a Z:Z(SPA-1) protein–protein complex. Uncomplexed Z(SPA-1) behaves as an aggregation-prone molten globule, but folding occurs on binding, and the original (Z) three-helix bundle scaffold is fully formed in the complex. The structural basis for selection and strong binding is a large interaction interface with tight steric and polar/nonpolar complementarity that directly involves 10 of 13 mutated amino acid residues on Z(SPA-1). We also note similarities in how the surface of the Z domain responds by induced fit to binding of Z(SPA-1) and Ig Fc, respectively, suggesting that the Z(SPA-1) affibody is capable of mimicking the morphology of the natural binding partner for the Z domain

Advantages and disadvantages of gloves

• Although gloves protect hands from hazardous environmental factors, there are problems associated with their frequent use. • Dirt, irritation, maceration, allergy, and lack of comfort are the most important problems for consideration. • Permeation of gloves, spillage onto the skin from gloves, and absorption through gloves are means for contamination and irritation. • Irritant dermatitis by polymeric gloves is common in the occupational setting and can be caused by the polymer itself, additives to the polymer, or glove powders. • Allergic contact dermatitis to glove material may be delayed (type-IV) allergy or immediate (type-I) allergy and is perhaps less common than irritant contact dermatitis. • Allergic sensitization is an ongoing concern as presence of allergens imposes on integral precautions within the healthcare setting such as preventing allergic reactions in patients during procedures and protection of patients and healthcare workers from biohazardous materials. • Allergic sensitization may impose upon an employee’s ability to perform tasks necessary for employment within the occupational setting