91 research outputs found
Impact of cyclic lean--rich aging under condition on the lean-gas light-off and hydrogen formation ability of a lean trap (LNT)
Uncoupled activation and cyclization in catmint reductive terpenoid biosynthesis
Terpene synthases typically form complex molecular scaffolds by concerted activation and cyclization of linear starting materials in a single enzyme active site. Here we show that iridoid synthase, an atypical reductive terpene synthase, catalyzes the activation of its substrate 8-oxogeranial into a reactive enol intermediate, but does not catalyze the subsequent cyclization into nepetalactol. This discovery led us to identify a class of nepetalactol-related short-chain dehydrogenase enzymes (NEPS) from catmint (Nepeta mussinii) that capture this reactive intermediate and catalyze the stereoselective cyclisation into distinct nepetalactol stereoisomers. Subsequent oxidation of nepetalactols by NEPS1 provides nepetalactones, metabolites that are well known for both insect-repellent activity and euphoric effect in cats. Structural characterization of the NEPS3 cyclase reveals that it binds to NAD+ yet does not utilize it chemically for a non-oxidoreductive formal [4 + 2] cyclization. These discoveries will complement metabolic reconstructions of iridoid and monoterpene indole alkaloid biosynthesis
Electrocatalytic Behavior of Pd and Pt Nanoislands Deposited onto 4,4′-Dithiodipyridine SAMs on Au(111)
Characteristics of the NO dielectric film with low pressure chemical vapor deposition in-situ nitridation
The Influence of Incarceration on Children at the Intersection of Parental Gender and Race/Ethnicity: A Focus on Child Living Arrangements
Mental Health Issues Among Pregnant Women in Correctional Facilities: A Systematic Review
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