A significant antihypertensive effect of Holarrhena floribunda supported by an exploratory phytochemical study

Introduction: Holarrhena floribunda (G.Don) T.Durand & Schinz is a tree that can grow up to 25 m with white latex in its organs. The bark of this plant is commonly used in traditional medicine to treat dysentery, diarrhea, diabetes, malaria, and high blood pressure. In this study, phytochemical groups were widely investigated on trunk bark as well as on leaves of H. floribunda harvested in Danyi (Togo). Antioxidant activity and antihypertensive properties of the plant extracts were also evaluated on sound guinea pigs. Methods: The hydro-ethanolic extract of the trunk bark was prepared and antioxidant activity was evaluated via the DPPH radical-scavenging and the ferric-reducing antioxidant power (FRAP) methods, and via the determination of the total phenolics content. This hydro-ethanolic extract was used to assess the plant extract effect on blood pressure of sound guinea pigs. Results: So, phytochemical screening revealed that H. floribunda contained most of the phytochemical groups. The 50% inhibitive concentration of that extract by DPPH was 29.80 ± 0.001 μg/mL. The equivalent of Fe2+ by FRAP was 1009.9 ± 0.6 μmol/g of dry extract. The content of phenolic compounds was 139 ± 0.053 mg GAE (gallic acid equivalents)/g of dry extract. Intravenous injection of the extract (10, 20, 40, 80 mg/kg) via the invasive method in guinea pigs under normal strain caused a decrease in diastolic blood pressure, systolic blood pressure, and so in mean arterial pressure (MAP) by dose cumulative and time-dependent. Conclusion: The results show that hydro-ethanolic extract of H. floribunda trunk bark has antihypertensive and antioxidant properties. This might be the reason for the use of that extract for the treatment of hypertension in traditional medicin

A significant antihypertensive effect of Holarrhena floribunda supported by an exploratory phytochemical study

Introduction: Holarrhena floribunda (G.Don) T.Durand & Schinz is a tree that can grow up to 25 m with white latex in its organs. The bark of this plant is commonly used in traditional medicine to treat dysentery, diarrhea, diabetes, malaria, and high blood pressure. In this study, phytochemical groups were widely investigated on trunk bark as well as on leaves of H. floribunda harvested in Danyi (Togo). Antioxidant activity and antihypertensive properties of the plant extracts were also evaluated on sound guinea pigs. Methods: The hydro-ethanolic extract of the trunk bark was prepared and antioxidant activity was evaluated via the DPPH radical-scavenging and the ferric-reducing antioxidant power (FRAP) methods, and via the determination of the total phenolics content. This hydro-ethanolic extract was used to assess the plant extract effect on blood pressure of sound guinea pigs. Results: So, phytochemical screening revealed that H. floribunda contained most of the phytochemical groups. The 50% inhibitive concentration of that extract by DPPH was 29.80 ± 0.001 μg/mL. The equivalent of Fe2+ by FRAP was 1009.9 ± 0.6 μmol/g of dry extract. The content of phenolic compounds was 139 ± 0.053 mg GAE (gallic acid equivalents)/g of dry extract. Intravenous injection of the extract (10, 20, 40, 80 mg/kg) via the invasive method in guinea pigs under normal strain caused a decrease in diastolic blood pressure, systolic blood pressure, and so in mean arterial pressure (MAP) by dose cumulative and time-dependent. Conclusion: The results show that hydro-ethanolic extract of H. floribunda trunk bark has antihypertensive and antioxidant properties. This might be the reason for the use of that extract for the treatment of hypertension in traditional medicine

Evaluation of portable LIBS and portable XRF in the frame of multi-elemental analysis of agricultural soils and plants

International audienc

Evaluation of portable LIBS and portable XRF in the frame of multi-elemental analysis of agricultural soils and plants

International audienc

Evaluation of portable LIBS and portable XRF in the frame of multi-elemental analysis of agricultural soils and plants

International audienceIntroduction Agronomic research has a growing interest in collecting faster, cheaper and still efficient information about agricultural matrices in order to achieve a better monitoring of soil quality and fertility1. Laser-induced breakdown spectroscopy (LIBS) and X-Ray fluorescence spectrometry (XRF) have been identified as high potential techniques for on-site multi-elemental analysis of agronomic matrices such as plants2 and soils3. Materials and Methods The study of agricultural soils and plants, more precisely the multi-elemental quantification of several chemical elements of interest (P, Mg, Fe, Al, Ca, K…), was realized using portable LIBS (Z300 – SCIAPS) and portable XRF (S1 TITAN 800 – Bruker) systems. 62 soil samples and 40 plant samples have been prepared as pressed pellets. Multivariate calibrations have been achieved for quantitative analysis of the LIBS spectra. Both XRF and LIBS quantitative analysis were compared to the concentration values determinate by ICP OES. Results and Discussion Good practices related to sample preparation and experimental conditions to achieve relevant LIBS/XRF measurements in laboratory will be discussed. For both LIBS and XRF measurements, the influence of environmental parameters has been examined in order to assess the robustness of on-site analysis. Finally, a first campaign of on-site LIBS and XRF analyses on agricultural soils and plants has been performed. Conclusion The evaluation in the laboratory of two portable instruments, LIBS and XRF, on soils and plant samples, have revealed that these two techniques are very sensitive to moisture. The results of the field measurements on wheat leaves and soil will be presented and discussed

Evaluation of portable LIBS and portable XRF in the frame of multi-elemental analysis of agricultural soils and plants

International audienceIntroduction Agronomic research has a growing interest in collecting faster, cheaper and still efficient information about agricultural matrices in order to achieve a better monitoring of soil quality and fertility1. Laser-induced breakdown spectroscopy (LIBS) and X-Ray fluorescence spectrometry (XRF) have been identified as high potential techniques for on-site multi-elemental analysis of agronomic matrices such as plants2 and soils3. Materials and Methods The study of agricultural soils and plants, more precisely the multi-elemental quantification of several chemical elements of interest (P, Mg, Fe, Al, Ca, K…), was realized using portable LIBS (Z300 – SCIAPS) and portable XRF (S1 TITAN 800 – Bruker) systems. 62 soil samples and 40 plant samples have been prepared as pressed pellets. Multivariate calibrations have been achieved for quantitative analysis of the LIBS spectra. Both XRF and LIBS quantitative analysis were compared to the concentration values determinate by ICP OES. Results and Discussion Good practices related to sample preparation and experimental conditions to achieve relevant LIBS/XRF measurements in laboratory will be discussed. For both LIBS and XRF measurements, the influence of environmental parameters has been examined in order to assess the robustness of on-site analysis. Finally, a first campaign of on-site LIBS and XRF analyses on agricultural soils and plants has been performed. Conclusion The evaluation in the laboratory of two portable instruments, LIBS and XRF, on soils and plant samples, have revealed that these two techniques are very sensitive to moisture. The results of the field measurements on wheat leaves and soil will be presented and discussed