Próba wzbogacenia pieczywa pszennego

Szymon T. Dziub

Building health safety of seniors in the city environment. Contextual potential of logistic management

Artykuł poświęcono rozważaniom na temat budowania bezpieczeństwa zdrowotnego seniorów w mieście. Autorzy wskazują na implikacje procesów demograficznych dla rozwoju miast i pokazują, jak te procesy wpływają na decyzje w zakresie budowania bezpiecznego środowiska życia seniorów. Potencjał zarządzania logistycznego upatrują w tworzeniu warunków do aktywnego starzenia się, ujawniając różne możliwości włączenia logistyki w rozwiązywanie problemów seniorów.The article is focuses on the consideration of building health security for seniors in the city. The authors point to the implications of demographic processes for urban development and show how these processes influence decisions in the area of building a safe environment for senior citizens. The potential of logistic management is seen in creating conditions for active aging, revealing various possibilities of including logistics in solving problems of seniors

TNFα-induced activation of NFκB protects against UV-induced apoptosis specifically in p53-proficient cells

The signaling pathways that depend on p53 or NFκB transcription factors are essential components of cellular responses to stress. In general, p53 is involved in either activation of cell cycle arrest or induction of apoptosis, while NFκB exerts mostly anti-apoptotic functions; both regulatory pathways apparently interfere with each other. Here we aimed to analyze the effects of NFκB activation on DNA damage-induced apoptosis, either p53-dependent or p53-independent, in a set of human cell lines. Four cell lines, HCT116 and RKO colon carcinoma, NCI-H1299 lung carcinoma and HL60 myeloblastoma, each of them in two congenic variants either containing or lacking transcriptionally competent p53, were used. Cells were incubated with TNFα cytokine to activate NFκB and then treated with ultraviolet or ionizing radiation to induce apoptosis, which was assessed by measurement of the sub-G1 cell fraction. We observed that treatment with TNFα resulted in a significant reduction in the frequency of apoptotic cells in UV-irradiated p53-proficient lines (with exception of the UV-resistant NCI-H1299 cells). This anti-apoptotic effect was lost when cells were pretreated with parthenolide, an inhibitor of NFκB activation. In marked contrast, TNFα-pretreatment of p53-deficient lines resulted in an increased frequency of apoptotic cells after UV irradiation (with exception of HL60 cells). Such anti- and pro-apoptotic influence of TNFα was less obvious in cells treated with ionizing radiation. The data clearly indicates functional interference of both signaling pathways upon the damage-induced apoptotic response, yet the observed effects are both cell type- and stimulus-specific

Wpływ zmian demograficznych na służbę krwi w Polsce – logistyka jako remedium na przyszłość

Demographic changes linked to an ageing population make it necessary to look for ways to prevent their effects in the blood service. One forward-looking tool is logistics, offering a range of solutions, both strategic and operational in nature. The article examines the usefulness of logistics in the Polish blood services.Zachodzące zmiany demograficzne związane ze starzeniem się społeczeństwa powodują konieczność poszukiwania sposobów zapobiegania ich skutkom w służbie krwi. Jednym z perspektywicznych narzędzi jest logistyka, oferująca szereg rozwiązań, mających charakter zarówno strategiczny jak i operacyjny. W artykule rozpatrywana jest użyteczność logistyki w polskiej służbie krwi

RRAD, IL4I1, CDKN1A, and SERPINE1 genes are potentially co-regulated by NF-κB and p53 transcription factors in cells exposed to high doses of ionizing radiation

Abstract Background The cellular response to ionizing radiation involves activation of p53-dependent pathways and activation of the atypical NF-κB pathway. The crosstalk between these two transcriptional networks include (co)regulation of common gene targets. Here we looked for novel genes potentially (co)regulated by p53 and NF-κB using integrative genomics screening in human osteosarcoma U2-OS cells irradiated with a high dose (4 and 10 Gy). Radiation-induced expression in cells with silenced TP53 or RELA (coding the p65 NF-κB subunit) genes was analyzed by RNA-Seq while radiation-enhanced binding of p53 and RelA in putative regulatory regions was analyzed by ChIP-Seq, then selected candidates were validated by qPCR. Results We identified a subset of radiation-modulated genes whose expression was affected by silencing of both TP53 and RELA, and a subset of radiation-upregulated genes where radiation stimulated binding of both p53 and RelA. For three genes, namely IL4I1, SERPINE1, and CDKN1A, an antagonistic effect of the TP53 and RELA silencing was consistent with radiation-enhanced binding of both p53 and RelA. This suggested the possibility of a direct antagonistic (co)regulation by both factors: activation by NF-κB and inhibition by p53 of IL4I1, and activation by p53 and inhibition by NF-κB of CDKN1A and SERPINE1. On the other hand, radiation-enhanced binding of both p53 and RelA was observed in a putative regulatory region of the RRAD gene whose expression was downregulated both by TP53 and RELA silencing, which suggested a possibility of direct (co)activation by both factors. Conclusions Four new candidates for genes directly co-regulated by NF-κB and p53 were revealed