6 research outputs found
Comparison of methods for isolation of bacterial and fungal DNA from human blood
The study aimed at optimization of DNA isolation from blood of representatives of four microbial
groups causing sepsis, i.e., Gram negative: Escherichia
coli, Gram positive: Staphylococcus aureus, yeast: Candida albicans, and filamentous fungus: Aspergillus fumigatus. Additionally, the five commercial kits for microbial
DNA isolation from the blood were tested. The developed
procedure of DNA isolation consisted of three consecutive
steps, i.e., mechanical disruption, chemical lysis, and
thermal lysis. Afterward, DNA was isolated from the previously prepared samples (erythrocyte lysis) with the use of
five commercial kits for DNA isolation. They were compared paying heed to detection limit, concentration, DNA
purity, and heme concentration in samples. The isolation of
DNA without preliminary erythrocyte lysis resulted in far
higher heme concentration than when lysis was applied. In
the variant with erythrocyte lysis, two of the commercial
kits were most effective in purifying the DNA extract from
heme. Designed procedure allowed obtaining microbial
DNA from all four groups of pathogens under study in the
amount sufficient to conduct the rtPCR reaction, which
aimed at detecting them in the blood
The Combined Estimator for Stochastic Equations on Graphs with Fractional Noise
In the present paper, we study the problem of estimating a drift parameter in stochastic evolution equations on graphs. We focus on equations driven by fractional Brownian motions, which are particularly useful e.g., in biology or neuroscience. We derive a novel estimator (the combined estimator) and prove its strong consistency in the long-span asymptotic regime with a discrete-time sampling scheme. The promising performance of the combined estimator for finite samples is examined under various scenarios by Monte Carlo simulations