Wildlife and parasitic infections: A One Health perspective in Greece

While research on the parasitic fauna of wildlife in Greece is currently limited, conducted studies have provided valuable insights into the prevalence of parasitic infections in wild carnivores, omnivores, and herbivores. This review consolidates the existing data on the endoparasites detected in wild animals in Greece, specifically focusing on those that pose established or potential zoonotic risks. Over the last 60 years, various parasite species such as Leishmania infantum, Cryptosporidium spp., Toxoplasma gondii, Sarcocystis spp., Toxocara canis, Ancylostoma caninum, Capillaria spp., Baylisascaris spp., Trichinella spp., Thelazia callipaeda, Dirofilaria immitis, Echinococcus granulosus, Mesocestoides sp., Taenia spp., Alaria alata, and Dicrocoelium dendriticum have been identified in wildlife in Greece. These findings have become increasingly relevant due to the growing interaction between humans and wild animals, which now extends to urban environments, as well as the increased contact between wild and domestic animals. This is further complicated by the geographical expansion of vector-borne diseases due to global warming and the increased movements of humans and animals. Surveillance and monitoring of parasitic infections in Greek wildlife is warranted, and it should be based on interdisciplinary investigations considering the interconnectedness of human, wild, and domestic animals, as well as environmental health, in line with the One Health approach

Current Applications of Digital PCR in Veterinary Parasitology: An Overview

Digital PCR (dPCR) is an emerging technology that enables the absolute quantification of the targeted nucleic acids. The body of research on the potential applications of this novel tool is growing in human and veterinary medicine. Most of the research on dPCR applications in veterinary parasitology is concentrated on developing and validating new assays to detect and quantify parasites of great financial impact in the food-producing animal industry. Several studies describe the utility of dPCR for individualized medicine in companion animals. Most frequently, dPCR performance is assessed compared to quantitative PCR or Next Generation Sequencing platforms, while others also compare the accuracy of dPCR with traditional parasitological techniques considered gold standard methods. Other researchers describe dPCR assays for surveillance purposes, species identification, and quantification in mixed parasitic infections, the detection of mutations indicative of anthelmintic resistance, and the identification of new targets for drug development. This review provides an overview of the studies that employed dPCR in investigating animal parasites and parasitic diseases from a veterinary perspective and discusses how this novel technology could advance and facilitate diagnosis, surveillance, and the monitoring of response to treatment, or shed light on current gaps in our knowledge of the epidemiology of significant veterinary parasitic diseases

Leishmaniosis in Greece: The Veterinary Perspective

Leishmaniosis caused by the protozoon Leishmania infantum that is transmitted through the bites of infected phlebotomine sandflies is of major veterinary concern in Greece. The country is endemic with particularly favourable environmental conditions for the spread of this infection. Moreover, Greece remains a popular touristic destination, and the continuous travel of pets raises concern regarding the possible dissemination of infection from endemic to non-endemic areas. Dogs are the main reservoir host, although other animal species, including humans, may also be infected. Canine leishmaniosis manifests as a visceral disease that can result in death if left untreated. Serological and molecular epizootiological studies have confirmed circulation of the parasite in Greek canine and feline populations as well as in other mammals. As a result, constant surveillance and identification of high-risk localities are necessary to establish chemoprophylactic protocols for travelling animals to safeguard animal and public health

Modified Beilschowsky silver impregnation combined with Hematoxylin or Cresyl Violet counterstaining as a potential tool for the simultaneous study of inflammation and axonal injury in the central nervous system

Background: Hematoxylin-Eosin (H&E), Cresyl Violet and Bielschowsky Silver Impregnation (BSI) are among the basic histological methods for the evaluation of inflammation and axonal injury in the Central Nervous System (CNS). The usual strategy so far is staining serial sections of CNS separately for H&E and BSI, whilst the simultaneous application of both methods on a single brain section could give more accurate information for both inflammatory and degenerative components. However, the classical protocols for BSI have unstable reproducibility, high staining background and, evidently, hematoxylin is not easily applied. Materials-Methods: Brain sections from Experimental Allergic Encephalomyelitis (EAE) and Traumatic Brain Injury (TBI) animal models were used. Different pH and incubation periods in the process of BSI staining were investigated to establish a reproducible protocol with minimal background. Results: The pH of the BSI working solutions strongly affect the final results of the method and the application of specific pHs almost eliminate background up to a point where H&E or cresyl violet counterstaining can easily be applied. Conclusion: The proposed combination is cost effective, has minimal background and increased reproducibility. It is proposed as a basic screening tool in the study of CNS disorders, namely those where inflammatory process and axonal pathology coexist