Phylogenetic and functional analysis of the Cation Diffusion Facilitator (CDF) family: improved signature and prediction of substrate specificity-0

<p><b>Copyright information:</b></p><p>Taken from "Phylogenetic and functional analysis of the Cation Diffusion Facilitator (CDF) family: improved signature and prediction of substrate specificity"</p><p>http://www.biomedcentral.com/1471-2164/8/107</p><p>BMC Genomics 2007;8():107-107.</p><p>Published online 23 Apr 2007</p><p>PMCID:PMC1868760.</p><p></p>t. Amino acid position refers to PtdMTP1. The hypothetical secondary structure (TMDs II and III) is shown below

Phylogenetic and functional analysis of the Cation Diffusion Facilitator (CDF) family: improved signature and prediction of substrate specificity-1

<p><b>Copyright information:</b></p><p>Taken from "Phylogenetic and functional analysis of the Cation Diffusion Facilitator (CDF) family: improved signature and prediction of substrate specificity"</p><p>http://www.biomedcentral.com/1471-2164/8/107</p><p>BMC Genomics 2007;8():107-107.</p><p>Published online 23 Apr 2007</p><p>PMCID:PMC1868760.</p><p></p>SD) until O.D. = 1. Serial dilutions were spotted on SD or on SD + 15 mM ZnCl(15 mM Zn). Photographs were taken after 6 days of growth at 30°C. For each mutation different yeast transformants were used and gave the same results. The amino acid substitutions along with their topological positions are shown on the left. EV: empty vector; PtdMTP1: wild type protein

Phylogenetic and functional analysis of the Cation Diffusion Facilitator (CDF) family: improved signature and prediction of substrate specificity-3

<p><b>Copyright information:</b></p><p>Taken from "Phylogenetic and functional analysis of the Cation Diffusion Facilitator (CDF) family: improved signature and prediction of substrate specificity"</p><p>http://www.biomedcentral.com/1471-2164/8/107</p><p>BMC Genomics 2007;8():107-107.</p><p>Published online 23 Apr 2007</p><p>PMCID:PMC1868760.</p><p></p>) regions are indicated for each cluster. When more than one metal is transported, the metal preference is reflected by the written order within these metals. For each group and for Zrg17-like and ZnT9-like clusters logos showing the conserved residues in TMDs II and V are shown on the right. A simplified phylogenetic tree representation, expressed as a dendogram using Zrg17-like cluster as outgroup, is shown on the left. B. Montanini, D. Blaudez, M. Chalot, unpublished data; D. Blaudez, M. Chalot, unpublished data; assessed by heterologous complementation; deduced by mutant phenotype or over-expression in homologous system; measured in reconstituted proteoliposomes or in everted membrane vesicles; indirect evidence; measured in oocytes; by referring to the CDF domain

Phylogenetic and functional analysis of the Cation Diffusion Facilitator (CDF) family: improved signature and prediction of substrate specificity-2

<p><b>Copyright information:</b></p><p>Taken from "Phylogenetic and functional analysis of the Cation Diffusion Facilitator (CDF) family: improved signature and prediction of substrate specificity"</p><p>http://www.biomedcentral.com/1471-2164/8/107</p><p>BMC Genomics 2007;8():107-107.</p><p>Published online 23 Apr 2007</p><p>PMCID:PMC1868760.</p><p></p>ith respect to metal specificity: blue is for zinc, green for iron/zinc, violet for manganese and brown for unknown specificity. The three CDF groups are surrounded with coloured boxes with the same colour-code. Bootstrap values are indicated for each cluster. The scale bar indicates an evolutionary distance of 0.1 amino acid substitution per site

Effect of Cultivars, Environment, and Year on Agronomic Traits, Biochemical Composition of Major Constituents (Lipids, Proteins, and Polysaccharides), and Rheological Properties of Seeds in <i>Brassica juncea</i>

In order to provide varieties of Brassica juncea that meet agronomic and technological qualities, it is necessary to define criteria that are predictive of the quality of the finished product. The objective of our study is to characterize the macromolecular components of the mustard seed in order to identify the compositional profile responsible for the technological quality of mustard pastes. A collection of cultivars that maximizes genetic and technological diversity were grown on three plots and over two years. Our results showed (i) the impact of the genetic and pedoclimatic factors on seed macromolecular components and on rheological properties estimated by measuring the flow in a Bostwick consistometer, and (ii) the potential role of seed storage proteins and cell wall polysaccharides in the mustard paste consistency. Data analyses allow us to propose new criteria for evaluating the technological quality of seeds and to identify interesting genotypes as candidates for future breeding programs