Quality and shelf life of vacuum- and nitrogen-packed dried barb fish (Puntius spp.)

Vacuum and modified atmosphere packaging are widely used for preserving food items including fish. In this context, the quality and shelf life of air- (control), vacuum- and nitrogen-packed dried barb fish (Puntius spp.) stored at ambient temperature were evaluated through biochemical and microbial analyses for 90 days. In most of the storage days, significantly lower values of moisture, total volatile base-nitrogen (TVB-N) and peroxide value (PV) were observed in the vacuum pack sample compared to control. However, the TVB-N, PV, total coliforms and faecal coliforms counts were within the acceptable limit in all groups. The total plate count (TPC) increased gradually with time from an initial value of 4.29 log CFUg–1. However, compared to control, significantly lower TPC were found on and after the 30th day of storage in vacuum and nitrogen pack samples. Considering the TPC value of 5 log CFUg–1, the shelf life was determined at approximately 15 days for control, 35 days for nitrogen pack and 45 days for vacuum pack samples. Therefore, the vacuum pack is considered the best packaging, which may be utilised by the processors to produce and retail the dried products with prolonged shelf life

A Cross Sectional Study on Knowledge and Attitude among Diabetic Patients about Diabetes and Its ComplicationsA Cross Sectional Study on Knowledge and Attitude among Diabetic Patients about Diabetes and Its Complications

Abstract: Backgroun

Exploring the effectiveness of flavone derivatives for treating liver diseases: Utilizing DFT, molecular docking, and molecular dynamics techniques

In exploring nature's potential in addressing liver-related conditions, this study investigates the therapeutic capabilities of flavonoids. Utilizing in silico methodologies, we focus on flavone and its analogs (1–14) to assess their therapeutic potential in treating liver diseases. Molecular change calculations using density functional theory (DFT) were conducted on these compounds, accompanied by an evaluation of each analog's physiochemical and biochemical properties. The study further assesses these flavonoids' binding effectiveness and locations through molecular docking studies against six target proteins associated with human cancer. Tropoflavin and taxifolin served as reference drugs. The structurally modified flavone analogs (1–14) displayed a broad range of binding affinities, ranging from -7.0 to -9.4 kcal mol⁻¹, surpassing the reference drugs. Notably, flavonoid (7) exhibited significantly higher binding affinities with proteins Nrf2 (PDB:1 × 2 J) and DCK (PDB:1 × 2 J) (-9.4 and -8.1 kcal mol⁻¹) compared to tropoflavin (-9.3 and -8.0 kcal mol⁻¹) and taxifolin (-9.4 and -7.1 kcal mol⁻¹), respectively. Molecular dynamics (MD) simulations revealed that the docked complexes had a root mean square deviation (RMSD) value ranging from 0.05 to 0.2 nm and a root mean square fluctuation (RMSF) value between 0.35 and 1.3 nm during perturbation. The study concludes that 5,7-dihydroxyflavone (7) shows substantial promise as a potential therapeutic agent for liver-related conditions. However, further validation through in vitro and in vivo studies is necessary.Key insights from this study include: • Screening of flavanols and their derivatives to determine pharmacological and bioactive properties using ADMET, molinspiration, and pass prediction analysis. • Docking of shortlisted flavone derivatives with proteins having essential functions. • Analysis of the best protein-flavonoid docked complexes using molecular dynamics simulation to determine the flavonoid's efficiency and stability within a system