Additional file 7: of Identification and therapeutic modulation of a pro-inflammatory subset of disease-associated-microglia in Alzheimer’s disease

Table S7. Changes in expression of AD-associated Blue, Magenta and Yellow microglial modules in aging WT and APP/PS1 mice. (XLSX 10 kb

Additional file 10: of Identification and therapeutic modulation of a pro-inflammatory subset of disease-associated-microglia in Alzheimer’s disease

3D viSNE representation of microglial modules: DAM (Red) and Homeostatic (Black) genes identified by single-cell RNAseq mapped to WGCNA modules. (HTML 3818 kb

Additional file 5: of Identification and therapeutic modulation of a pro-inflammatory subset of disease-associated-microglia in Alzheimer’s disease

Table S5. Enrichment analysis of GWAS-identified human AD-risk genes in mouse microglial modules. (XLSX 22 kb

Additional file 2: of Identification and therapeutic modulation of a pro-inflammatory subset of disease-associated-microglia in Alzheimer’s disease

Table S2. Gene Ontology (GO) analyses of Blue, Magenta, Yellow and Midnightblue mouse microglial modules. (XLSX 110 kb

Additional file 9: of Identification and therapeutic modulation of a pro-inflammatory subset of disease-associated-microglia in Alzheimer’s disease

3D viSNE representation of microglial modules. Color is indicative of the respective module color. (HTML 3835 kb

Additional file 3: of Identification and therapeutic modulation of a pro-inflammatory subset of disease-associated-microglia in Alzheimer’s disease

Table S3. Identification of potential transcriptional regulators of mouse microglial modules. (XLSX 13 kb

Additional file 4: of Identification and therapeutic modulation of a pro-inflammatory subset of disease-associated-microglia in Alzheimer’s disease

Table S4. Putative membrane-associated drug targets and regulators of Blue, Magenta, Yellow and Midnightblue microglial transcriptomic modules. (XLSX 12 kb

Additional file 1: of Identification and therapeutic modulation of a pro-inflammatory subset of disease-associated-microglia in Alzheimer’s disease

Table S1. Mouse microglial transcriptomic modules identified by Weighted Correlation Network Analysis (WGCNA). (XLSX 5372 kb

Additional file 6: of Identification and therapeutic modulation of a pro-inflammatory subset of disease-associated-microglia in Alzheimer’s disease

Table S6. Top AD risk genes in Blue, Magenta and Yellow AD-associated microglial modules. (XLSX 22 kb

Additional file 1: Figures S1–S6. of A systems pharmacology-based approach to identify novel Kv1.3 channel-dependent mechanisms in microglial activation

Flow cytometric confirmation of Kv1.3-channel specificity of the ShK-F6CA binding assay is shown in Figure S1. Comparison of ShK-F6CA labeling of Kv1.3 channels in splenic and brain-infiltrating macrophages is shown in Figure S2. Morphological changes induced by LPS, ShK-223, and LPS+ShK-223 treatment conditions are shown in Figure S3. Distribution of missing data in the proteomic data set is shown in Figure S4. Quantitative RT-PCR data showing validation of pro-inflammatory activation of BV2 microglia by LPS are shown in Figure S5. EHD1 upregulation by LPS and inhibition of EHD1 upregulation by ShK-223 is shown in Figure S6. (DOCX 1262 kb