Role Emerging Placements: Skills Development, Postgraduate Employment, and Career Pathways

Occupational therapy educators are increasingly using role emerging placements (REPs) as a forum for students to develop skills required to work in emerging areas of practice. This study explores the impact of REPs on skill development, postgraduate employment, and career pathways for occupational therapists. An online survey was sent to occupational therapists across Canada (n = 1,763). Occupational therapists who had completed a REP responded to the online survey (n = 88). Descriptive analysis was used to examine trends in the quantitative data, and content analysis was used to code categories derived from qualitative survey data. Results indicated five skills that developed in REPs and were used throughout an occupational therapist’s career. REPs appeared to have no impact on choice of practice field postgraduation, career pathways, or employment status. However, a group who identified their current job titles other than occupational therapy indicated a positive experience regarding their skills, career pathways, and employment status. Study results highlight the need to further understand the experiences of graduates in their REPs and the factors in REPs that may influence the career trajectory of occupational therapists

Investigation of an extensive outbreak of HIV infection among children in Sindh, Pakistan: protocol for a matched case-control study.

INTRODUCTION: In April 2019, 14 children were diagnosed with HIV infection by a private healthcare provider in Larkana district, Sindh province, Pakistan. Over the next 3 months, 930 individuals were diagnosed with HIV, >80% below 16 years, the largest ever outbreak of HIV in children in Pakistan. In this protocol paper, we describe research methods for assessing likely modes of HIV transmission in this outbreak and investigate spatial and molecular epidemiology. METHODS AND ANALYSIS: A matched case-control study will be conducted with 406 cases recruited. Cases will be children aged below 16 years registered for care at the HIV treatment centre at Shaikh Zayed Children Hospital in Larkana City. Controls will be children who are HIV-uninfected (confirmed by a rapid HIV test) matched 1:1 by age (within 1 year), sex and neighbourhood. Following written informed consent from the guardian, a structured questionnaire will be administered to collect data on sociodemographic indices and exposure to risk factors for parenteral, vertical and sexual (only among those aged above 10 years) HIV transmission. A blood sample will be collected for hepatitis B and C serology (cases and controls) and HIV lineage studies (cases only). Mothers of participants will be tested for HIV to investigate the possibility of mother-to-child transmission. Conditional logistic regression will be used to investigate the association of a priori defined risk factors with HIV infection. Phylogenetic analyses will be conducted. Global positioning system coordinates of participants' addresses will be collected to investigate concordance between the genetic and spatial epidemiology. ETHICS AND DISSEMINATION: Ethical approval was granted by the Ethics Review Committee of the Aga Khan University, Karachi. Study results will be shared with Sindh and National AIDS Control Programs, relevant governmental and non-governmental organisations, presented at national and international research conferences and published in international peer-reviewed scientific journals

Fibrin glue in managing intractable gingival bleeding in patients with inherited bleeding disorders-a quasi-experimental pilot study

This study presents the clinical outcomes of using inhouse prepared fibrin glue for controlling gingival bleeding in patients with inherited bleeding disorders (IBD). The objective of the study was to assess the reduction in transfusion days and improvement in compliance for dental evaluation over a one-year period in a low-to-middle-income country. The quasiexperimental pilot study included 40 IBD patients with gingival bleeding. These were divided into two groups: Group A received fibrin glue (n=20), while Group B did not (n=20). The study compared outcome metrics, including the number of treatment days and blood components transfused, using non-parametric tests with a significance threshold of p\u3c0.05. Results showed that Group A required fewer blood components (n=154) as compared to Group B (n=204) (p\u3c0.001). Patients in Group A with Glanzmann thrombasthenia (GT) had a shorter treatment duration (one day) than those in group B (three days) (p\u3c0.01). In conclusion, the application of fibrin glue effectively managed intractable gingival bleeding in IBD patients

Phytochemical Studies and Antimicrobial Screening of Non/Less- Polar Fraction of Psoralea corylifolia by Using GC-MS

Psoralea corylifolia is a well-known medicinal plant, traditionally used against several diseased conditions. The present study was conducted to investigate the phytochemical composition and antimicrobial activity of P. corylifolia seeds. Non/less-polar fraction of methanolic seed extract was subjected to gas chromatography-mass spectrometry (GC-MS) for phytochemical analysis. A total of fourteen compounds were identified which include aromatic, sesquiterpenes, furocoumarins, sterols, fatty acid and their methyl esters. The predominant compounds were epoxycaryophyllene (3), isopsoralen (6), psoralen (7) and bakuchiol (9). Identification of these compounds was also strongly supported by Kovat’s Retention Indices. Furthermore, the n-hexane soluble fraction showed significant antimicrobial activity against several bacterial strains. P. corylifolia seeds represented a unique chemical composition with considerable antimicrobial activity which not only validates their traditional medicinal uses but also indicates their potential as a source of natural antimicrobial compounds

Phytochemical Studies and Antimicrobial Screening of Non/Less-Polar Fraction of Psoralea corylifolia by Using GC-MS

Psoralea corylifolia is a well-known medicinal plant, traditionally used against several diseased conditions. The present study was conducted to investigate the phytochemical composition and antimicrobial activity of P. corylifolia seeds. Non/less-polar fraction of methanolic seed extract was subjected to gas chromatography-mass spectrometry (GC-MS) for phytochemical analysis. A total of fourteen compounds were identified which include aromatic, sesquiterpenes, furocoumarins, sterols, fatty acid and their methyl esters. The predominant compounds were epoxycaryophyllene (3), isopsoralen (6), psoralen (7) and bakuchiol (9). Identification of these compounds was also strongly supported by Kovat’s Retention Indices. Furthermore, the n-hexane soluble fraction showed significant antimicrobial activity against several bacterial strains. P. corylifolia seeds represented a unique chemical composition with considerable antimicrobial activity which not only validates their traditional medicinal uses but also indicates their potential as a source of natural antimicrobial compounds

Effect of roasting and frying treatments on aflatoxins and capsaicinoids content and nutritional profile of green chilies (Capsicum annum L.)

Abstract This study was conducted to assess the effect of two thermal treatments, viz. roasting and pan frying (deep frying), on nutritional profile, aflatoxin, and capsaicin content in green chilies. Green chilies were subjected to roasting and frying to reduce the aflatoxin contamination, besides retaining their pungency and nutritional profile. Reversed‐phase HPLC was employed to determine the levels of aflatoxins B1, B2, G1, and G2 in thermally treated and control samples. The proximate compositions of roasted and fried chili samples were significantly (p ≤ .05) different from raw chili (control), except ash content. Vitamin A levels decreased significantly (p ≤ .05) during roasting and were undetected in fried chili samples. Likewise, vitamin C was undetected in both roasted and fried chili samples. Significantly decreasing (p ≤ .05) trends were noticed in capsaicinoids viz. capsaicin and dihydrocapsaicin contents including Scoville Heat Units (SHU) during roasting and frying. However, retention of capsaicinoids was higher in roasted chilies (730.00 ± 4.90 mg/kg) than fried samples (502.56 ± 5.10 mg/kg). The levels of all the four major aflatoxins (AFs)‐ AFB1, AFB2, AFG1, and AFG2 recorded in control were much higher than the limits prescribed by the European Union for spices. Both thermal treatments (roasting and frying) employed proved to be effective in reducing aflatoxins like AFB2, AFG1, and AFG2 in chilies to below the prescribed limits, while as the level of AFB1 was reduced below the limits by only the frying method. This study therefore indicated the substantial impact of frying on aflatoxins